FHK12: Difference between revisions
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Created page with "{| class="wikitable" border="1" |- | Name | FHK12 |- | Synonyms | |- | Genotype | F' lacIq lacZΔM15 proA+B+ ara Δ(lac-proAB) rpsL (ϕ80dlacZΔM15) attB::(ctx::lacZ) (source:..." |
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| [https://bccm.belspo.be/ | | [https://bccm.belspo.be/page/genecorner-hosts-display-razor/684 LMBP 11202] | ||
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| References | | References | ||
Latest revision as of 11:23, 28 October 2024
| Name | FHK12 |
| Synonyms | |
| Genotype | F' lacIq lacZΔM15 proA+B+ ara Δ(lac-proAB) rpsL (ϕ80dlacZΔM15) attB::(ctx::lacZ) (source: Kolmar et al., 1995) |
| Phenotype | ampicillin and streptomycin resistant |
| Sources | LMBP 11202 |
| References | PMID:7664730 |
Notes
The ctx::lacZ fusion integrated at the attB site is tagged with the ampicillin resistance gene.
This FHK12 strain was constructed as follows:
First FHK11 was constructed from strain JM83 (Yanish-Perron et al., 1985) by chromosomal integration of the lacZ gene under control of the V. cholerae ctxAB promoter from plasmid pLDR10-ctx::lacZ at the phage λ attB site (Diederich et al., 1992).