DH5alpha F(λ): Difference between revisions
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Created page with "{| class="wikitable" border="1" |- | Name | DH5alpha F(λ) |- | Synonyms | DH5αF(λ) |- | Genotype | F+ Δ(argF-lac)169 φ80dlacZ58(M15) ΔphoA8 glnX44(AS) λ+ deoR481 rfbC1..." |
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| Sources | | Sources | ||
| [https://bccm.belspo.be/ | | [https://bccm.belspo.be/page/genecorner-hosts-display-razor/165 LMBP 2846] | ||
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| References | | References | ||
Latest revision as of 11:22, 28 October 2024
| Name | DH5alpha F(λ) |
| Synonyms | DH5αF(λ) |
| Genotype | F+ Δ(argF-lac)169 φ80dlacZ58(M15) ΔphoA8 glnX44(AS) λ+ deoR481 rfbC1 gyrA96 recA1 endA1 thiE1 hsdR17 |
| Phenotype | nalidixic acid resistant |
| Sources | LMBP 2846 |
| References |
Notes
Useful strain for Lacα complementation. This strain does not contain the lacIq gene and therefore repression on the lac promoter, present on high-copy plasmids, is incomplete.
This strain contains the F factor of NK3 and therefore can be used for infection with M13 or fd phages and derivatives. In addition the strain is lysogenic for wild-type phage λ.