Category:Strain:H12R8a: Difference between revisions
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[http://cgsc.biology.yale.edu/Strain.php?ID=11297 CGSC:2597]<br /> | [http://cgsc.biology.yale.edu/Strain.php?ID=11297 CGSC:2597]<br /> | ||
[ | [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%204980 LMBP 4980] | ||
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!align=left {{table_heading_style}} |Construction | !align=left {{table_heading_style}} |Construction | ||
Latest revision as of 13:20, 16 October 2020
See Help:Strains for more information about strains in EcoliWiki.
| Name |
H12R8a |
|---|---|
| Synonyms | |
| Genotype |
garB10 fhuA22 phoA5(Am) ompF627(T2R) tyrT34(AS) fadL701(T2R) relA1 pitA10 spoT1 phoT-35 rrnB-2 mcrB1 creC510 |
| Phenotype |
phosphatase positive |
| Plasmids |
Hfr |
| Phage |
λ+ |
| Sources | |
| Construction |
Strain H12R8a is a spontaneous isolate of strain H12 |
| References |
PMID:5327650[1] PMID:4552408[2] PMID:4564480[3] PMID:4286570[4] |
| edit table |
Notes
This strain carries the H12 amber mutation (TAG) in the phosphatase gene.
This strain carries the supF suppressor (inserts tyrosine at the amber position) and is therefore capable of suppressing the H12 phosphatase nonsense mutation, as well as amber mutations in other genes. However, the efficiency of suppression may vary considerably depending on the sequence context of the amber codon.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Garen, A et al. (1965) Suppressor genes for nonsense mutations. I. The Su-1, Su-2 and Su-3 genes of Escherichia coli. J. Mol. Biol. 14 167-78 PubMed
- ↑ Shimada, K et al. (1972) Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J. Mol. Biol. 63 483-503 PubMed
- ↑ Remaut, E & Fiers, W (1972) Studies on the bacteriophage MS2. XVI. The termination signal of the A protein cistron. J. Mol. Biol. 71 243-61 PubMed
- ↑ Weigert, MG et al. (1965) Amino acid substitutions resulting from suppression of nonsense mutations. II. Glutamine insertion by the Su-2 gene; tyrosine insertion by the Su-3 gene. J. Mol. Biol. 14 522-7 PubMed
Pages in category "Strain:H12R8a"
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