BL21(DE3): Difference between revisions
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{ | {| class="wikitable" border="1" | ||
|- | |||
| Name | |||
| BL21(DE3) | |||
|- | |||
| Synonyms | |||
| | |||
|- | |||
| Genotype | |||
| ''E. coli B dcm ompT hsdS''(r<sub>B</sub><sup>-</sup>m<sub>B</sub><sup>-</sup>) ''gal'' | |||
|- | |||
| Phenotype | |||
|- | |||
| Phage | |||
| [[lambda DE3|λDE3]] | |||
|- | |||
| Sources | |||
| [https://bccm.belspo.be/catalogues/genecorner-host-details?NUM=LMBP%201455 LMBP 1455] | |||
|- | |||
| References | |||
| [https://www.ncbi.nlm.nih.gov/pubmed/3537305 PMID: 3537305] | |||
|} | |||
===Notes=== | |||
The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase. Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs. | The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase. Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs. | ||
The strain does not contain the lon protease and is also deficient in the outer membrane protease, OmpT. The lack of two key proteases reduces the degradation of heterologous proteinsexpressed in the strain | The strain does not contain the lon protease and is also deficient in the outer membrane protease, OmpT. The lack of two key proteases reduces the degradation of heterologous proteinsexpressed in the strain. | ||
[[Category: | [[Category:BCCM/GeneCorner]][[Category:Strains]] | ||
[[Category: | |||
Revision as of 11:28, 16 October 2020
| Name | BL21(DE3) |
| Synonyms | |
| Genotype | E. coli B dcm ompT hsdS(rB-mB-) gal |
| Phenotype | |
| Phage | λDE3 |
| Sources | LMBP 1455 |
| References | PMID: 3537305 |
Notes
The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase. Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs. The strain does not contain the lon protease and is also deficient in the outer membrane protease, OmpT. The lack of two key proteases reduces the degradation of heterologous proteinsexpressed in the strain.