DB3.1: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
|||
| Line 12: | Line 12: | ||
| Phenotype | | Phenotype | ||
| Streptomycin resistant, ccdB resistant | | Streptomycin resistant, ccdB resistant | ||
|- | |- | ||
| Sources | | Sources | ||
| [http://bccm.belspo.be/catalogues/files/lmbp-hosts/db3-1.pdf LMBP 4098] | | [http://bccm.belspo.be/catalogues/files/lmbp-hosts/db3-1.pdf LMBP 4098] | ||
|- | |- | ||
| References | | References | ||
Revision as of 08:55, 4 July 2016
| Name | DB3.1 |
| Synonyms | |
| Genotype | gyrA462 endA1 ∆(sr1-recA) mcrB mrr hsdS20 glnV44 (=supE44) ara14 galK2 lacY1 proA2 rpsL20 xyl5 leuB6 mtl1 |
| Phenotype | Streptomycin resistant, ccdB resistant |
| Sources | LMBP 4098 |
| References | PMID:1324324 |
Notes
DB3.1 is a HB101 derivative containing the gyrA462 allele which renders the strain resistant to the toxic effects of the ccdB gene.
Original Invitrogen catalogue number: 11782-018. However, this strain is no longer available at Invitrogen.
Alternative: ccdB-resistant One Shot® ccdB Survival™ 2 T1R Competent Cells, of which the ccdB-resistance mechanism is not known; it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent.