HB101: Difference between revisions

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==Phenotypes ==
==Phenotypes ==
Streptomycin resistance
Streptomycin resistance
Lacks the K-12 restriction-modification system


==Construction ==
==Construction ==

Revision as of 07:50, 14 November 2015

See Help:Strains for more iformation about strains in EcoliWiki.


Alternate Names

Sources

Chromosomal Genotype

Different sources have different genotypes for HB101. The genotype listed below is from the Coli Genetics Stock Center.

F- Lambda- araC14 leuB6(Am) DE(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) recA13 rpsL20(strR) xylA5 mtl-1 thiE1 hsdS20(rB-, mB-)[1]

NEB: includes DE(mcrC-mrr) Promega (and others): proA2

Plasmids

Phage

Phenotypes

Streptomycin resistance

Lacks the K-12 restriction-modification system

Construction

Notes

Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.

Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56.

Lacks S and Greenberg JR (1977) J Mol Biol 114:153.

References

See Help:References for how to manage references in EcoliWiki.