HB101: Difference between revisions
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==Chromosomal Genotype== | ==Chromosomal Genotype== | ||
Different sources have different genotypes for HB101. | Different sources have different genotypes for HB101. The genotype listed below is from the Coli Genetics Stock Center. | ||
F<sup>-</sup> Lambda<sup>-</sup> araC14 leuB6(Am) DE(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) recA13 rpsL20(strR) xylA5 mtl-1 thiE1 hsdS20(r<sub>B</sub><sup>-</sup>, m<sub>B</sub><sup>-</sup>)<ref>http://cgsc.biology.yale.edu/Strain.php?ID=130465</ref> | F<sup>-</sup> Lambda<sup>-</sup> araC14 leuB6(Am) DE(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) recA13 rpsL20(strR) xylA5 mtl-1 thiE1 hsdS20(r<sub>B</sub><sup>-</sup>, m<sub>B</sub><sup>-</sup>)<ref>http://cgsc.biology.yale.edu/Strain.php?ID=130465</ref> | ||
NEB: includes DE(mcrC-mrr) | |||
Promega (and others): proA2 | |||
==Plasmids == | ==Plasmids == | ||
Revision as of 07:48, 14 November 2015
See Help:Strains for more iformation about strains in EcoliWiki.
Alternate Names
Sources
Chromosomal Genotype
Different sources have different genotypes for HB101. The genotype listed below is from the Coli Genetics Stock Center.
F- Lambda- araC14 leuB6(Am) DE(gpt-proA)62 lacY1 glnX44(AS) galK2(Oc) recA13 rpsL20(strR) xylA5 mtl-1 thiE1 hsdS20(rB-, mB-)[1]
NEB: includes DE(mcrC-mrr) Promega (and others): proA2
Plasmids
Phage
Phenotypes
Streptomycin resistance
Construction
Notes
Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.
Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56.
Lacks S and Greenberg JR (1977) J Mol Biol 114:153.
References
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