Addgene

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Link/URL:	www.Addgene.org
What:	Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) Click to search vector database now.
Who:	Addgene is a nonprofit global plasmid repository dedicated to helping scientists share their plasmids.
Updates:	Do you use plasmids? Join the non-profit plasmid sharing community. Visit www.addgene.org to learn more, find plasmids, and start depositing!
Upcoming events:	Visit Addgene's News and Announcements page to see current news and announcements.
Web Services:	Addgene is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.
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About Addgene

Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.

Content

Plasmid Repository

In 2014 Addgene's repository distributes 30,000 types of plasmids, deposited by 1,700 labs. Addgene’s plasmid collection contains plasmids used for a variety of functions such as genome engineering (including CRISPRS), gene expression, shRNA knockdown, viral-mediated gene delivery, detection of miRNA and promoter activity.

The plasmid collection includes:

• Genome engineering
  • CRISPRs
  • Transcription Activator-Like Effector Nuclease (TALEN) kits
  • Zinc finger nuclease kits
• Empty backbones
  • Species-specific expression
  • Epitope tags
  • Fusion proteins
  • Selectable markers
  • Fluorescent marker
• Viral vectors
  • Retroviral/Lentiviral
  • Adenoviral
  • Adeno-associated virus
• cDNA expression
• shRNA expression

Tools and guides

Molecular Biology Tools

Vector Database- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

Sequence Analyzer- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

Molecular Biology Reference- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

Plasmid Cloning Guides

Molecular Cloning Guides- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

Molecular Cloning Protocols- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

Using Addgene

Browsing

Browse Plasmid Repository by plasmid type, source, bacterial resistance, and selectable marker.

Browse By Plasmid Type:
Mammalian Expression	Bacterial Expression
Luciferase	Mouse Targeting
Retroviral	Lentiviral
Worm Expression	Insect Expression
Yeast Expression, E.Coli or yeast	RNAi
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Searching

Additional Searches By:
Bacterial Resistance	Ampicillin; Kanamycin; Tetracycline; Chloramphenicol; Hygromycin; Spectinomycin; Gentamycin
Selectable Marker	Neomycin; Puromycin; Hygromycin; Zeocin; URA3; LEU2; TRP1;HIS3
Source	Addgene; ATCC; BD Biosciences; Invitrogen; Novagen; Sigma; Stratagene
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