PMID:2022622: Difference between revisions
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!align=left |Citation | !align=left |Citation | ||
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'''Shah, S and Peterkofsky, A''' (1991) Characterization and generation of Escherichia coli adenylate cyclase deletion mutants.''J. Bacteriol.'' '''173''':3238-42 | '''Shah, S and Peterkofsky, A''' (1991) Characterization and generation of Escherichia coli adenylate cyclase deletion mutants. ''J. Bacteriol.'' '''173''':3238-42 | ||
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!align=left |Abstract | !align=left |Abstract | ||
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2022622 PubMed] | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2022622 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC207922 PMC207922] | ||
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!align=left |Keywords | !align=left |Keywords | ||
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Adenylate Cyclase; Base Sequence; Chromosome Deletion; DNA Mutational Analysis; DNA, Bacterial; Escherichia coli; Genes, Bacterial; Genetic Vectors; Molecular Sequence Data; Polymerase Chain Reaction; Restriction Mapping | Adenylate Cyclase/genetics; Base Sequence; Chromosome Deletion; DNA Mutational Analysis; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Genetic Vectors; Molecular Sequence Data; Polymerase Chain Reaction; Restriction Mapping | ||
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
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Latest revision as of 04:36, 15 September 2013
| Citation |
Shah, S and Peterkofsky, A (1991) Characterization and generation of Escherichia coli adenylate cyclase deletion mutants. J. Bacteriol. 173:3238-42 |
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| Abstract |
Escherichia coli delta cya-283 is a 75-bp in-frame deletion overlapping the 5' end of delta cya-854; delta cya-201 is a 41-bp frameshift deletion overlapping the 3' end of delta cya-854. Sequence repeats were found at the boundaries of delta cya-283 and delta cya-201, suggesting a mechanism for deletion formation. Recombinant DNA procedures were used to construct a strain in which the total cya structural gene in the chromosome was replaced by the kanamycin resistance gene. |
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| Keywords |
Adenylate Cyclase/genetics; Base Sequence; Chromosome Deletion; DNA Mutational Analysis; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Genetic Vectors; Molecular Sequence Data; Polymerase Chain Reaction; Restriction Mapping |
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