PMID:2022622
| Citation |
Shah, S and Peterkofsky, A (1991) Characterization and generation of Escherichia coli adenylate cyclase deletion mutants. J. Bacteriol. 173:3238-42 |
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| Abstract |
Escherichia coli delta cya-283 is a 75-bp in-frame deletion overlapping the 5' end of delta cya-854; delta cya-201 is a 41-bp frameshift deletion overlapping the 3' end of delta cya-854. Sequence repeats were found at the boundaries of delta cya-283 and delta cya-201, suggesting a mechanism for deletion formation. Recombinant DNA procedures were used to construct a strain in which the total cya structural gene in the chromosome was replaced by the kanamycin resistance gene. |
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| Keywords |
Adenylate Cyclase/genetics; Base Sequence; Chromosome Deletion; DNA Mutational Analysis; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Genetic Vectors; Molecular Sequence Data; Polymerase Chain Reaction; Restriction Mapping |
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