PMID:9829956: Difference between revisions

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<span style = 'font-size: large'> '''Nichols, BP, Shafiq, O and Meiners, V'''  (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction.''J. Bacteriol.'' '''180''':6408-11</span>
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!align=left  |Citation
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'''No author listed'''  (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. ''J. Bacteriol.'' '''180''':6408-11
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!align=left  |Abstract
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The chromosomal insertion sites of Tn10-containing Escherichia coli strains were amplified by inverse PCR, and the nucleotide sequences of the junctions were determined. In 95 strains analyzed, 88 unique Tn10 positions were determined and matched to the E. coli chromosome sequence. Two gaps in insertion site positions were noted, one including the terminus of DNA replication and another bounded by recombination hot spots RhsA and RhsB.
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!align=left  |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9829956 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC107733 PMC107733]


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|{{TableHeadingColor}} |'''Abstract''' || The chromosomal insertion sites of Tn10-containing Escherichia coli strains were amplified by inverse PCR, and the nucleotide sequences of the junctions were determined. In 95 strains analyzed, 88 unique Tn10 positions were determined and matched to the E. coli chromosome sequence. Two gaps in insertion site positions were noted, one including the terminus of DNA replication and another bounded by recombination hot spots RhsA and RhsB.
!align=left  |Keywords
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Base Sequence; Binding Sites/genetics; Chromosome Mapping/methods; Chromosomes, Bacterial/genetics; DNA Primers/genetics; DNA Transposable Elements/genetics; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes, Bacterial; Open Reading Frames; Polymerase Chain Reaction
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9829956 PubMed]
 
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==Significance {{HelpIcon|Help:Literature}} ==
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
{{LitSignificance}}

Revision as of 20:09, 1 September 2013

Citation

No author listed (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180:6408-11

Abstract

The chromosomal insertion sites of Tn10-containing Escherichia coli strains were amplified by inverse PCR, and the nucleotide sequences of the junctions were determined. In 95 strains analyzed, 88 unique Tn10 positions were determined and matched to the E. coli chromosome sequence. Two gaps in insertion site positions were noted, one including the terminus of DNA replication and another bounded by recombination hot spots RhsA and RhsB.

Links

PubMed PMC107733

Keywords

Base Sequence; Binding Sites/genetics; Chromosome Mapping/methods; Chromosomes, Bacterial/genetics; DNA Primers/genetics; DNA Transposable Elements/genetics; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes, Bacterial; Open Reading Frames; Polymerase Chain Reaction

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