PMID:2581811: Difference between revisions

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<span style = 'font-size: large'> '''Kawakami, M, Miyazaki, M, Yamada, H and Mizushima, S'''  (1985) Isolation of gram quantities of isoleucyl-tRNA synthetase from an overproducing strain of Escherichia coli and its use for purification of cognate tRNA.''FEBS Lett.'' '''185''':162-4</span>
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|{{TableHeadingColor}} |'''Abstract''' || <protect>The ileS gene coding for isoleucyl-tRNA synthetase was cloned on a runaway-replication plasmid. From the cells harboring the plasmid, gram quantities of the synthetase were isolated using two column procedures. The synthetase was used for the purification of cognate tRNA. Isoleucine tRNAGAU of greater than 90% purity was easily isolated by taking advantage of a specific complex formation of the synthetase with cognate tRNA.</protect>
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'''No author listed''' (1985) Isolation of gram quantities of isoleucyl-tRNA synthetase from an overproducing strain of Escherichia coli and its use for purification of cognate tRNA. ''FEBS Lett.'' '''185''':162-4
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!align=left  |Abstract
*  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2581811 PubMed]
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The ileS gene coding for isoleucyl-tRNA synthetase was cloned on a runaway-replication plasmid. From the cells harboring the plasmid, gram quantities of the synthetase were isolated using two column procedures. The synthetase was used for the purification of cognate tRNA. Isoleucine tRNAGAU of greater than 90% purity was easily isolated by taking advantage of a specific complex formation of the synthetase with cognate tRNA.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2581811 PubMed]
 
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Amino Acyl-tRNA Synthetases/isolation & purification; Chromatography; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Isoleucine-tRNA Ligase/isolation & purification; Isoleucine-tRNA Ligase/metabolism; Plasmids; RNA, Bacterial/isolation & purification; RNA, Transfer, Amino Acyl/isolation & purification; RNA, Transfer, Amino Acyl/metabolism


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==Significance {{HelpIcon|Help:Literature}} ==
==Significance {{HelpIcon|Help:Literature}} ==
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{{LitSignificance}}

Revision as of 19:18, 1 September 2013

Citation

No author listed (1985) Isolation of gram quantities of isoleucyl-tRNA synthetase from an overproducing strain of Escherichia coli and its use for purification of cognate tRNA. FEBS Lett. 185:162-4

Abstract

The ileS gene coding for isoleucyl-tRNA synthetase was cloned on a runaway-replication plasmid. From the cells harboring the plasmid, gram quantities of the synthetase were isolated using two column procedures. The synthetase was used for the purification of cognate tRNA. Isoleucine tRNAGAU of greater than 90% purity was easily isolated by taking advantage of a specific complex formation of the synthetase with cognate tRNA.

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PubMed

Keywords

Amino Acyl-tRNA Synthetases/isolation & purification; Chromatography; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Isoleucine-tRNA Ligase/isolation & purification; Isoleucine-tRNA Ligase/metabolism; Plasmids; RNA, Bacterial/isolation & purification; RNA, Transfer, Amino Acyl/isolation & purification; RNA, Transfer, Amino Acyl/metabolism

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