PMID:17543954

Eydallin, G, Viale, AM, Morán-Zorzano, MT, Muñoz, FJ, Montero, M, Baroja-Fernández, E and Pozueta-Romero, J (2007) Genome-wide screening of genes affecting glycogen metabolism in Escherichia coli K-12.FEBS Lett. 581:2947-53

Abstract

<protect>A systematic and comprehensive gene-disrupted mutant collection of E. coli K-12 was used to identify genes whose deletions affect glycogen accumulation. Of the 3985 non-essential gene mutants of the collection, 35 displayed a glycogen-excess phenotype, whereas 30 displayed either glycogen-less or glycogen-deficient phenotypes. The genes whose deletions affect glycogen accumulation were classified into various functional categories, including energy production, envelope composition and integrity, protein translation and stability, transport of inorganic ions and nucleotides, and metabolism of carbohydrates and amino acids. The overall data indicate that glycogen metabolism is highly interconnected with a wide variety of cellular processes in E. coli.

|- |bgcolor='#CCCCFF' nowrap |Links || PubMed
Online version:10.1016/j.febslet.2007.05.044 |} </protect>

Significance

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Useful Materials and Methods

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The Keio collection was subjected to an iodine staining technique as a screening method for glycogen content in solid glucose Kornberg medium. Iodine vapor exposure causes glycogen-excess mutants to stain darker than parental cells, and glycogen-deficient mutants to stain lighter .

65 mutants were discovered to have quantitative glycogen levels of significant difference from the wild type. Gene deletion mutants resulted in glycogen-excess phenotypes (35 total) and glycogen-deficient/less phenotypes (30 total). See figures 1 and 2 for reporting of quantitative glycogen content as a percentage of glycogen accumulated by the wild type cells.

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
clpP	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
cspC	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
cysA	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
cysD	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
cysE	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
cysG	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
cysI	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
cysJ	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
cysK	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
cysN	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
cysP	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
cysU	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
cysW	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
deoT	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
dsbA	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
dsbB	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
fdx	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
galU	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
glgA	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-less	complete
glgB	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-less	complete
glgC	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-less	complete
glgP	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
glgX	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
glnD	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
greA	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
hfq	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
iscS	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
iscU	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
lon	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
mdtH	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
mgtA	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
miaA	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
nlpD	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
nudB	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
nusB	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
phoB	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
phoP	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
phoQ	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
pgm	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-less	complete
proW	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
pstA	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
pstC	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
pstS	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
purC	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
purH	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
pyrE	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
rbfA	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
rfaH	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
rnt	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
rpmJ	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
rpoN	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
rpoS	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
rpsF	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
serA	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
serB	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
serC	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
ubiG	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-less	complete
ybhE	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
yciU	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
ydcS	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
ygdD	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
yhbG	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
ynfA	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
yobG	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-excess	complete
yqgB	Contributes to	GO:0005978	glycogen biosynthetic process	IMP: Inferred from mutant phenotype		P	Glycogen-deficient	complete
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