PMID:17543954
| Citation |
Eydallin, G, Viale, AM, Morán-Zorzano, MT, Muñoz, FJ, Montero, M, Baroja-Fernández, E and Pozueta-Romero, J (2007) Genome-wide screening of genes affecting glycogen metabolism in Escherichia coli K-12. FEBS Lett. 581:2947-53 |
|---|---|
| Abstract |
A systematic and comprehensive gene-disrupted mutant collection of E. coli K-12 was used to identify genes whose deletions affect glycogen accumulation. Of the 3985 non-essential gene mutants of the collection, 35 displayed a glycogen-excess phenotype, whereas 30 displayed either glycogen-less or glycogen-deficient phenotypes. The genes whose deletions affect glycogen accumulation were classified into various functional categories, including energy production, envelope composition and integrity, protein translation and stability, transport of inorganic ions and nucleotides, and metabolism of carbohydrates and amino acids. The overall data indicate that glycogen metabolism is highly interconnected with a wide variety of cellular processes in E. coli. |
| Links |
PubMed Online version:10.1016/j.febslet.2007.05.044 |
| Keywords |
Amino Acids/metabolism; Bacterial Outer Membrane Proteins/metabolism; Carbohydrate Metabolism/genetics; Energy Metabolism/genetics; Escherichia coli K12/genetics; Escherichia coli K12/metabolism; Gene Deletion; Genes, Bacterial; Genome, Bacterial; Genomics/methods; Glycogen/metabolism |
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The Keio collection[1] was subjected to an iodine staining technique as a screening method for glycogen content in solid glucose Kornberg medium. Iodine vapor exposure causes glycogen-excess mutants to stain darker than parental cells, and glycogen-deficient mutants to stain lighter[2] .
65 mutants were discovered to have quantitative glycogen levels of significant difference from the wild type. Gene deletion mutants resulted in glycogen-excess phenotypes (35 total) and glycogen-deficient/less phenotypes (30 total). See figures 1 and 2[3] for reporting of quantitative glycogen content as a percentage of glycogen accumulated by the wild type cells.
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- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ Govons, S et al. (1969) Isolation of mutants of Escherichia coli B altered in their ability to synthesize glycogen. J. Bacteriol. 97 970-2 PubMed
- ↑ Eydallin, G et al. (2007) Genome-wide screening of genes affecting glycogen metabolism in Escherichia coli K-12. FEBS Lett. 581 2947-53 PubMed