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<span style = 'font-size: large'> '''Lee, JY, Yang, KS, Jang, SA, Sung, BH and Kim, SC''' (2011) Engineering butanol-tolerance in escherichia coli with artificial transcription factor libraries.''Biotechnol. Bioeng.'' '''108''':742-9</span> | |||
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|{{TableHeadingColor}} |'''Abstract''' || <protect>Escherichia coli has been explored as a host for butanol production because of its many advantages such as a fast growth and easy genetic manipulation. Butanol toxicity, however, is a major concern in the biobutanol production with E. coli. In particular, E. coli growth is severely inhibited by butanol, being almost completely stopped by 1% (vol/vol) butanol. Here we developed a new method to increase the butanol-tolerance of E. coli with artificial transcription factor (ATF) libraries which consist of zinc finger (ZF) DNA-binding proteins and an E. coli cyclic AMP receptor protein (CRP). Using these ATFs, we selected a butanol-tolerant E. coli which can tolerate up to 1.5% (vol/vol) butanol, with a concomitant increase in heat resistance. We also identified genes of E. coli that are associated with the butanol-tolerance. These results show that E. coli can be engineered as a promising host for high-yield butanol production.</protect> | |||
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* [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=21404248 PubMed] | |||
Online version:[http://dx.doi.org/10.1002/bit.22989 10.1002/bit.22989] | |||
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Revision as of 23:45, 9 August 2011
Lee, JY, Yang, KS, Jang, SA, Sung, BH and Kim, SC (2011) Engineering butanol-tolerance in escherichia coli with artificial transcription factor libraries.Biotechnol. Bioeng. 108:742-9
| Abstract | <protect>Escherichia coli has been explored as a host for butanol production because of its many advantages such as a fast growth and easy genetic manipulation. Butanol toxicity, however, is a major concern in the biobutanol production with E. coli. In particular, E. coli growth is severely inhibited by butanol, being almost completely stopped by 1% (vol/vol) butanol. Here we developed a new method to increase the butanol-tolerance of E. coli with artificial transcription factor (ATF) libraries which consist of zinc finger (ZF) DNA-binding proteins and an E. coli cyclic AMP receptor protein (CRP). Using these ATFs, we selected a butanol-tolerant E. coli which can tolerate up to 1.5% (vol/vol) butanol, with a concomitant increase in heat resistance. We also identified genes of E. coli that are associated with the butanol-tolerance. These results show that E. coli can be engineered as a promising host for high-yield butanol production. |
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Online version:10.1002/bit.22989 |} </protect>
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