PMID:21404248
| Citation |
Lee, JY, Yang, KS, Jang, SA, Sung, BH and Kim, SC (2011) Engineering butanol-tolerance in escherichia coli with artificial transcription factor libraries. Biotechnol. Bioeng. 108:742-9 |
|---|---|
| Abstract |
Escherichia coli has been explored as a host for butanol production because of its many advantages such as a fast growth and easy genetic manipulation. Butanol toxicity, however, is a major concern in the biobutanol production with E. coli. In particular, E. coli growth is severely inhibited by butanol, being almost completely stopped by 1% (vol/vol) butanol. Here we developed a new method to increase the butanol-tolerance of E. coli with artificial transcription factor (ATF) libraries which consist of zinc finger (ZF) DNA-binding proteins and an E. coli cyclic AMP receptor protein (CRP). Using these ATFs, we selected a butanol-tolerant E. coli which can tolerate up to 1.5% (vol/vol) butanol, with a concomitant increase in heat resistance. We also identified genes of E. coli that are associated with the butanol-tolerance. These results show that E. coli can be engineered as a promising host for high-yield butanol production. |
| Links |
PubMed Online version:10.1002/bit.22989 |
| Keywords |
Butanols/metabolism; Cyclic AMP Receptor Protein/genetics; Cyclic AMP Receptor Protein/metabolism; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Genes, Bacterial; Genetic Engineering/methods; Hot Temperature; Industrial Microbiology/methods; Zinc Fingers |
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