BW25113: Difference between revisions
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==Construction == | ==Construction == | ||
BW25113 is a derivative of the F<sup>-</sup>, λ<sup>-</sup>, ''E. coli'' K-12 strain BD792 [http://cgsc2.biology.yale.edu/Strain.php?ID=7024 CGSC6159]<ref name='PMID:10829079'/>. According to the CGSC, BD792 | BW25113 is a derivative of the F<sup>-</sup>, λ<sup>-</sup>, ''E. coli'' K-12 strain BD792 [http://cgsc2.biology.yale.edu/Strain.php?ID=7024 CGSC6159]<ref name='PMID:10829079'/>. According to the CGSC, BD792 carries ''rpoS396''(Am), but this mutation is not listed in the genotype for BW25113. | ||
==Notes== | ==Notes== | ||
Revision as of 20:24, 22 June 2011
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Alternate Names
Chromosomal Genotype
∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514
Plasmids
Phage
Phenotypes
Construction
BW25113 is a derivative of the F-, λ-, E. coli K-12 strain BD792 CGSC6159[1]. According to the CGSC, BD792 carries rpoS396(Am), but this mutation is not listed in the genotype for BW25113.
Notes
BW25113 was used to make the Keio Collection of single-gene knockouts. [2] [1] Available from the Coli Genetics Stock Center: CGSC#7636 The genotype for BW25113 in Datsenko et al. [1] said the strain is lacIQ, but it was later shown to be lacI+ Baba et al. (Supplementary Table 1)[2].
According to the CGSC, lacZ4787(del) has 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
References
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- ↑ 1.0 1.1 1.2 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed