pBAD24: Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
Table edited by Azweifel via TableEdit
Line 36: Line 36:
!align=left {{table heading style}} |Features
!align=left {{table heading style}} |Features
||
||
*AraC
*''araC''
*optimized SD sequence
*translation start codon
*Kozak sequence (for eukaryotic expression)
|-
|-
!align=left {{table heading style}} |Accession Number
!align=left {{table heading style}} |Accession Number

Revision as of 22:18, 15 July 2010

See Help:Plasmid for more information about plasmids in EcoliWiki.


At a Glance

Type

Cloning Expression Vector

Origin of Replication
  • pBR322/ColE1
  • M13
Copy #
Markers

AmpR

Link to Sequence
Features
  • araC
  • optimized SD sequence
  • translation start codon
  • Kozak sequence (for eukaryotic expression)
Accession Number

X81838


Notes

  • used to clone gene which lack sequences for initiation of translation

History

Construction described in Guzman et al. (1995)[1].

Sources

You can help EcoliWiki by writing about the available sources of this plasmid.

References

See Help:References for how to manage references in EcoliWiki.

  1. ↑ Guzman, LM et al. (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177 4121-30 PubMed

Sequence

You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.