Hi-Control(tm) 10G: Difference between revisions
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==Chromosomal Genotype== | ==Chromosomal Genotype== | ||
F- <em>mcrA</em> Δ(<em>mrr-hsd</em>RMS-<em>mcr</em>BC) <em>end</em>A1 <em>rec</em>A1 Φ80<em>dlac</em>ZΔM15 Δ<em>lac</em>X74 <em>ara</em>D139 Δ(<em>ara,leu</em>)7697 <em>gal</em>U <em>gal</em>K <em>rps</em>L <em>nup</em>G λ- <em>ton</em>A | |||
==Plasmids== | ==Plasmids== | ||
Mini-F <em>lacI<sup>q1</sup></em>(Gent<sup>r</sup>) | |||
==Phage== | ==Phage== | ||
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==Notes== | ==Notes== | ||
*The HI-Control 10G cells contain a single-copy BAC plasmid harboring a specially engineered version of the <em>lacI<sup>q1</sup></em> repressor allele. The <em>lacI<sup>q1</sup></em> allele expresses ~170-fold more lac repressor protein than the wild-type <em>lacI</em> gene. | |||
*For stable cloning of T7 protein expression plasmids. | |||
*Resistant to phage T1. | |||
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__NOTOC__ | __NOTOC__ | ||
<noinclude> | |||
[[Category:Strains]] | |||
[[Category:Cloning Strains]] | |||
</noinclude> | |||
Revision as of 19:23, 13 July 2010
See Help:Strains for more information about strains in EcoliWiki.
Alternate Names
Sources
Chromosomal Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
Plasmids
Mini-F lacIq1(Gentr)
Phage
Phenotypes
Construction
Notes
- The HI-Control 10G cells contain a single-copy BAC plasmid harboring a specially engineered version of the lacIq1 repressor allele. The lacIq1 allele expresses ~170-fold more lac repressor protein than the wild-type lacI gene.
- For stable cloning of T7 protein expression plasmids.
- Resistant to phage T1.
References
See Help:References for how to manage references in EcoliWiki.