PMID:20375161: Difference between revisions
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==Significance {{HelpIcon|Help:Literature}} == | ==Significance {{HelpIcon|Help:Literature}} == | ||
{{LitSignificance}} | {{LitSignificance}} | ||
This paper is significant to the study of bacteriophage lambda and closely related phages because it demonstrates that immunity to superinfection in lambda is not absolute, though the mechanism for this is likely not via integrase mediated site specific recombination but rather homologous recombination within the resident prophage. This is in stark contrast to other bacteriophages deemed to be lambdoid, which may actively promote integration of superinfecting phages into distinct sites throughout the genome at apparantly high frequencies. Therefore, the assumption that all lambdoid phages must exactly abide by the canonical rules, based on lambda itself, is unfounded. | |||
== Useful Materials and Methods {{HelpIcon|Help:Literature}}== | == Useful Materials and Methods {{HelpIcon|Help:Literature}}== | ||
Revision as of 23:59, 21 April 2010
Fogg, PC, Allison, HE, Saunders, JR and McCarthy, AJ (2010) Bacteriophage lambda - a paradigm revisited.J Virol
| Abstract | <protect>Bacteriophage lambda has an archetypal immunity system, which prevents superinfection of its E. coli lysogens. It is now known that superinfection at high frequency can occur with toxigenic lambda-like phages, and here we demonstrate that superinfection of a lambda lysogen can lead to acquisition of additional lambda genomes, confirmed by Southern hybridisation and quantitative PCR. As many as 8 integration events were observed, but at very low frequency (6.4 x 10(-4)) and always as multiple insertions at the established primary integration site in E. coli. Sequence analysis of the complete immunity region demonstrated that these multiply infected lysogens were not immunity mutants. In conclusion, although lambda superinfection immunity can be confounded, it is a rare event. |
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|bgcolor='#CCCCFF' nowrap |Links || PubMed
Online version:10.1128/JVI.02177-09
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Significance
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This paper is significant to the study of bacteriophage lambda and closely related phages because it demonstrates that immunity to superinfection in lambda is not absolute, though the mechanism for this is likely not via integrase mediated site specific recombination but rather homologous recombination within the resident prophage. This is in stark contrast to other bacteriophages deemed to be lambdoid, which may actively promote integration of superinfecting phages into distinct sites throughout the genome at apparantly high frequencies. Therefore, the assumption that all lambdoid phages must exactly abide by the canonical rules, based on lambda itself, is unfounded.
Useful Materials and Methods
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