BW25113: Difference between revisions
Jump to navigation
Jump to search
imported>Wikientrybot Make better help links. |
DanielRenfro (talk | contribs) Undo revision 754732 by Wikientrybot (Talk) |
||
| Line 1: | Line 1: | ||
{{ | ==Alternate Names {{HelpIcon|Help:Strains}}== | ||
==Chromosomal Genotype {{HelpIcon|Help:Strains}}== | |||
==Chromosomal Genotype == | |||
∆''(araD-araB)567'', ∆''lacZ4787''(::rrnB-3), lambda<sup>-</sup>, ''rph-1'', ∆''(rhaD-rhaB)568'', ''hsdR514'' | ∆''(araD-araB)567'', ∆''lacZ4787''(::rrnB-3), lambda<sup>-</sup>, ''rph-1'', ∆''(rhaD-rhaB)568'', ''hsdR514'' | ||
==Plasmids == | ==Plasmids {{HelpIcon|Help:Strains}}== | ||
==Phage == | ==Phage {{HelpIcon|Help:Strains}}== | ||
==Phenotypes == | ==Phenotypes {{HelpIcon|Help:Strains}}== | ||
==Construction == | ==Construction {{HelpIcon|Help:Strains}}== | ||
==Notes== | ==Notes== | ||
Revision as of 19:41, 25 February 2009
Alternate Names
Chromosomal Genotype
∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514
Plasmids
Phage
Phenotypes
Construction
Notes
BW25113 was used to make the Keio Collection of single-gene knockouts. [1] [2] Available from the Coli Genetics Stock Center: CGSC#7636 The genotype for BW25113 in Datsenko et al. [2] said the strain is lacIQ, but it was later shown to be lacI+ (Supplementary Table 1)[1].
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 2.0 2.1 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed