DH10B: Difference between revisions
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useful for generating genomic libraries containing methylated cytosine or adenine residues<br /> | useful for generating genomic libraries containing methylated cytosine or adenine residues<br /> | ||
useful for plasmid rescue procedures<br /> | useful for plasmid rescue procedures<br /> | ||
Copied from GIBCO | |||
== References {{HelpIcon|Help:Strains}}== | == References {{HelpIcon|Help:Strains}}== | ||
{{RefHelp}}<references/> | {{RefHelp}}<references/> | ||
__NOTOC__ | __NOTOC__ | ||
[[Category:Strains]] | [[Category:Strains]] | ||
Revision as of 20:21, 11 November 2008
Alternate Names
The sequence of DH10B was published in 2008[1] .
DH10B
Sources
Chromosomal Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λ-
From J Jessee.[2]
Plasmids
not specified
Phage
not specified
Phenotypes
Construction
unknown parents.
Comments
host for pUC and other α-complementation vectors; pBR322
useful for generating genomic libraries containing methylated cytosine or adenine residues
useful for plasmid rescue procedures
Copied from GIBCO
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
- ↑ Grant, SG et al. (1990) Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants. Proc. Natl. Acad. Sci. U.S.A. 87 4645-9 PubMed