PMID:6998946: Difference between revisions
Table edited by Bmcintosh via TableEdit |
Table edited by Bmcintosh via TableEdit |
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As shown in Table 2, mutants of adh have increased levels of alcohol dehydrogenase activity under aerobic conditions. | As shown in Table 2, mutants of adh have increased levels of alcohol dehydrogenase activity under aerobic conditions. | ||
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complete | |||
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Revision as of 21:33, 3 July 2008
Clark, DP and Cronan, JE (1980) Acetaldehyde coenzyme A dehydrogenase of Escherichia coli.J. Bacteriol. 144:179-84
| Abstract | Mutants of Escherichia coli (adh) in which alcohol dehydrogenase is derepressed under aerobic conditions were also found to overproduce acetaldehyde coenzyme a dehydrogenase. However, acetaldehyde coenzyme A dehydrogenase was induced by ethanol or acetaldehyde and subject to strong catabolite repression, whereas alcohol dehydrogenase was little affected by these conditions. Mutants no longer able to use ethanol as carbon source were isolated from an adh strain. Some of these mutants were revertants at the adh locus and no longer produced either alcohol dehydrogenase or acetaldehyde coenzyme A dehydrogenase. Others, designated acd, were found to lack only acetaldehyde coenzyme A dehydrogenase. The acd mutation was located at min 62 of the E. coli genetic map, the gene order being thyA-lysA-acd-serA-fda. Isolation of Tn10 insertions cotransducible with acd greatly simplified the mapping procedure. |
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Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
AdhE |
GO:0004022 |
alcohol dehydrogenase activity |
IMP: Inferred from Mutant Phenotype |
F |
As shown in Table 2, mutants of adh have increased levels of alcohol dehydrogenase activity under aerobic conditions. |
complete | ||
|
AdhE |
GO:0008774 |
acetaldehyde dehydrogenase (acetylating) activity |
IMP: Inferred from Mutant Phenotype |
F |
Mutants of adh were shown to have increased acetaldehyde coenzyme A dehydrogenase in aerobic conditions compared to wild-type cells (Table 2). |
complete | ||
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