pCC1BAC: Difference between revisions
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==At a Glance | ==At a Glance== | ||
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==History {{HelpIcon|Help:Plasmid}}== | ==History {{HelpIcon|Help:Plasmid}}== | ||
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Revision as of 17:34, 24 October 2007
At a Glance
| Type |
Cloning |
|---|---|
| Origin of Replication |
single-copy F-factor, multi-copy oriV |
| Copy # |
single copy and can be induced to 10-20 copies per cell |
| Markers | |
| Link to Sequence | |
| Comments |
Copy Control Vector; For oriV to function as an origin of replication, oriV requires a gene product called "trfA". Promega has genetically engineered E.coli strains to have the trfA gene under the control of a tightly-regulated inducible promoter. Each Cloning Ready pCC1BAC Vector has been linearized at a unique BamH 1, EcoR 1 or Hind III site. |
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History
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Sources
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References
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Sequence
You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.