pCC1BAC: Difference between revisions
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!{{table heading style}} |Type | !{{table heading style}} |Type | ||
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Cloning | |||
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!{{table heading style}} |Origin of Replication | !{{table heading style}} |Origin of Replication | ||
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single-copy F-factor, multi-copy oriV | |||
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!{{table heading style}} |Copy # | !{{table heading style}} |Copy # | ||
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single copy and can be induced to 10-20 copies per cell | |||
|- | |- | ||
!{{table heading style}} |Markers | !{{table heading style}} |Markers | ||
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!{{table heading style}} |Link to Sequence | !{{table heading style}} |Link to Sequence | ||
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=157065011 PubMed] | |||
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!{{table heading style}} |Comments | !{{table heading style}} |Comments | ||
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Copy Control Vector; | |||
For oriV to function as an origin of replication, oriV requires a gene product called "trfA". Promega has genetically engineered E.coli strains to have the trfA gene under the control of a tightly-regulated inducible promoter. Each Cloning Ready pCC1BAC Vector has been linearized at a unique BamH 1, EcoR 1 or Hind III site. | |||
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Revision as of 17:34, 24 October 2007
At a Glance
| Type |
Cloning |
|---|---|
| Origin of Replication |
single-copy F-factor, multi-copy oriV |
| Copy # |
single copy and can be induced to 10-20 copies per cell |
| Markers | |
| Link to Sequence | |
| Comments |
Copy Control Vector; For oriV to function as an origin of replication, oriV requires a gene product called "trfA". Promega has genetically engineered E.coli strains to have the trfA gene under the control of a tightly-regulated inducible promoter. Each Cloning Ready pCC1BAC Vector has been linearized at a unique BamH 1, EcoR 1 or Hind III site. |
| edit table | |
History
You can help EcoliWiki by writing about the history of this plasmid.
Sources
You can help EcoliWiki by writing about the available sources of this plasmid.
References
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Sequence
You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.