pLacI

At a Glance

Type	Expression
Origin of Replication	p15a
Copy #
Markers	Chloramphenicol
Link to Sequence	Novagen
Comments	The pLacI plasmid encodes the lac repressor protein. The p15a origin of replication is compatible with colE1 based vectors.
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History

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Sources

Novagen

References

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Sequence

   1  GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG

  51  GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA

 101  TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC

 151  CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC

 201  CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT

 251  AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT

 301  GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC

 351  AGGGCTTCCC GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG

 401  TGATCTTCCG TCACAGGTAT TTATTCGGCG CAAAGTGCGT CGGGTGATGC

 451  TGCCAACTTA CTGATTTAGT GTATGATGGT GTTTTTGAGG TGCTCCAGTG

 501  GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG ACGGGGTGGT

 551  GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT

 601  GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG

 651  CAGGAGAAAA AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA

 701  TATATTCCGC TTCCTCGCTC ACTGACTCGC TACGCTCGGT CGTTCGACTG

 751  CGGCGAGCGG AAATGGCTTA CGAACGGGGC GGAGATTTCC TGGAAGATGC

 801  CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA AGCCGTTTTT

 851  CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC

 901  AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCTG

 951  GCGGCTCCCT CGTGCGCTCT CCTGTTCCTG CCTTTCGGTT TACCGGTGTC

1001  ATTCCGCTGT TATGGCCGCG TTTGTCTCAT TCCACGCCTG ACACTCAGTT

1051  CCGGGTAGGC AGTTCGCTCC AAGCTGGACT GTATGCACGA ACCCCCCGTT

1101  CAGTCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC

1151  GGAAAGACAT GCAAAAGCAC CACTGGCAGC AGCCACTGGT AATTGATTTA

1201  GAGGAGTTAG TCTTGAAGTC ATGCGCCGGT TAAGGCTAAA CTGAAAGGAC

1251  AAGTTTTGGT GACTGCGCTC CTCCAAGCCA GTTACCTCGG TTCAAAGAGT

1301  TGGTAGCTCA GAGAACCTTC GAAAAACCGC CCTGCAAGGC GGTTTTTTCG

1351  TTTTCAGAGC AAGAGATTAC GCGCAGACCA AAACGATCTC AAGAAGATCA

1401  TCTTATTAAT CAGATAAAAT ATTTCTAGAT TTCAGTGCAA TTTATCTCTT

1451  CAAATGTAGC ACCTGAAGTC AGCCCCATAC GATATAAGTT GTAATTCTCA

1501  TGTTAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT

1551  CTCAAGGGCA TCGGTCGAGA TCCCGGTGCC TAATGAGTGA GCTAACTTAC

1601  ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT

1651  GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT

1701  ATTGGGCGCC AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT

1751  GATTGCCCTT CACCGCCTGG CCCTGAGAGA GTTGCAGCAA GCGGTCCACG

1801  CTGGTTTGCC CCAGCAGGCG AAAATCCTGT TTGATGGTGG TTAACGGCGG

1851  GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT ACCGAGATGT

1901  CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC

1951  GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT

2001  CAGCATTTGC ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT

2051  CCCGTTCCGC TATCGGCTGA ATTTGATTGC GAGTGAGATA TTTATGCCAG

2101  CCAGCCAGAC GCAGACGCGC CGAGACAGAA CTTAATGGGC CCGCTAACAG

2151  CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG CCCAGTCGCG

2201  TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG

2251  ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT

2301  GGCATCCTGG TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT

2351  GCGCGAGAAG ATTGTGCACC GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT

2401  TCTACCATCG ACACCACCAC GCTGGCACCC AGTTGATCGG CGCGAGATTT

2451  AATCGCCGCG ACAATTTGCG ACGGCGCGTG CAGGGCCAGA CTGGAGGTGG

2501  CAACGCCAAT CAGCAACGAC TGTTTGCCCG CCAGTTGTTG TGCCACGCGG

2551  TTGGGAATGT AATTCAGCTC CGCCATCGCC GCTTCCACTT TTTCCCGCGT

2601  TTTCGCAGAA ACGTGGCTGG CCTGGTTCAC CACGCGGGAA ACGGTCTGAT

2651  AAGAGACACC GGCATACTCT GCGACATCGT ATAACGTTAC TGGTTTCACA

2701  TTCACCACCC TGAATTGACT CTCTTCCGGG CGCTATCATG CCATACCGCG

2751  AAAGGTTTTG CGCCATTCGA TGGTGTCCGG GATCTCGACG CTCTCCCTTA

2801  TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG

2851  CACCGCCGCC GCAAGGAATG GTGTCGTCGC CGCACTTATG ACTGTCTTCT

2901  TTATCATGCA ACTCGTAGGA CAGGTGCCGG CAGCGCCCAA CAGTCCCCCG

2951  GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC CCTGCACCAT

3001  TATGTTCCGG ATCTGCATCG CAGGATGCTG CTGGCTACCC TGTGGAACAC

3051  CTACATCTGT ATTAACGAAG CGCTAACCGT TTTTATCAGG CTCTGGGAGG

3101  CAGAATAAAT GATCATATCG TCAATTATTA CCTCCACGGG GAGAGCCTGA

3151  GCAAACTGGC CTCAGGCATT TGAGAAGCAC ACGGTCACAC TGCTTCCGGT

3201  AGTCAATAAA CCGGTAAACC AGCAATAGAC ATAAGCGGCT ATTTAACGAC

3251  CCTGCCCTGA ACCGACGACC GGGTCGAATT TGCTTTCGAA TTTCTGCCAT

3301  TCATCCGCTT ATTATCACTT ATTCAGGCGT AGCACCAGGC GTTTAAGGGC

3351  ACCAATAACT GCCTTAAAAA AATTACGCCC CGCCCTGCCA CTCATCGCAG

3401  TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAGAC

3451  GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG

3501  TATAATATTT GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG

3551  GCCACGTTTA AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC

3601  GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC

3651  CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG

3701  TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA

3751  AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT

3801  TCATTGCCAT ACG