pLacI
At a Glance
pLacI Image made with PlasMapper
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication |
p15a |
| Copy # | |
| Markers |
Chloramphenicol |
| Link to Sequence | |
| Features |
LacI coding sequence |
| Accession Number |
|
| edit table |
</protect>
Notes
The pLacI plasmid encodes the lac repressor protein. The p15a origin of replication is compatible with colE1 based vectors, which contain lac operators that regulate T7 promoter-driven expression but do not supply the lac repressor. Co-transformation with pLacI thus supplies a source of lac repressor protein to maintain repression of these vectors in lDE3 lysogenic bacterial expression host strains. Expression of lacI is driven by a constitutive E. coli promoter.
History
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Sources
References
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Sequence
1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG 51 GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA 101 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC 151 CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC 201 CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT 251 AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT 301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC 351 AGGGCTTCCC GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG 401 TGATCTTCCG TCACAGGTAT TTATTCGGCG CAAAGTGCGT CGGGTGATGC 451 TGCCAACTTA CTGATTTAGT GTATGATGGT GTTTTTGAGG TGCTCCAGTG 501 GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG ACGGGGTGGT 551 GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT 601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG 651 CAGGAGAAAA AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA 701 TATATTCCGC TTCCTCGCTC ACTGACTCGC TACGCTCGGT CGTTCGACTG 751 CGGCGAGCGG AAATGGCTTA CGAACGGGGC GGAGATTTCC TGGAAGATGC 801 CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA AGCCGTTTTT 851 CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC 901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCTG 951 GCGGCTCCCT CGTGCGCTCT CCTGTTCCTG CCTTTCGGTT TACCGGTGTC 1001 ATTCCGCTGT TATGGCCGCG TTTGTCTCAT TCCACGCCTG ACACTCAGTT 1051 CCGGGTAGGC AGTTCGCTCC AAGCTGGACT GTATGCACGA ACCCCCCGTT 1101 CAGTCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 1151 GGAAAGACAT GCAAAAGCAC CACTGGCAGC AGCCACTGGT AATTGATTTA 1201 GAGGAGTTAG TCTTGAAGTC ATGCGCCGGT TAAGGCTAAA CTGAAAGGAC 1251 AAGTTTTGGT GACTGCGCTC CTCCAAGCCA GTTACCTCGG TTCAAAGAGT 1301 TGGTAGCTCA GAGAACCTTC GAAAAACCGC CCTGCAAGGC GGTTTTTTCG 1351 TTTTCAGAGC AAGAGATTAC GCGCAGACCA AAACGATCTC AAGAAGATCA 1401 TCTTATTAAT CAGATAAAAT ATTTCTAGAT TTCAGTGCAA TTTATCTCTT 1451 CAAATGTAGC ACCTGAAGTC AGCCCCATAC GATATAAGTT GTAATTCTCA 1501 TGTTAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT 1551 CTCAAGGGCA TCGGTCGAGA TCCCGGTGCC TAATGAGTGA GCTAACTTAC 1601 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 1651 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT 1701 ATTGGGCGCC AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT 1751 GATTGCCCTT CACCGCCTGG CCCTGAGAGA GTTGCAGCAA GCGGTCCACG 1801 CTGGTTTGCC CCAGCAGGCG AAAATCCTGT TTGATGGTGG TTAACGGCGG 1851 GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT ACCGAGATGT 1901 CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC 1951 GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT 2001 CAGCATTTGC ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT 2051 CCCGTTCCGC TATCGGCTGA ATTTGATTGC GAGTGAGATA TTTATGCCAG 2101 CCAGCCAGAC GCAGACGCGC CGAGACAGAA CTTAATGGGC CCGCTAACAG 2151 CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG CCCAGTCGCG 2201 TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG 2251 ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT 2301 GGCATCCTGG TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT 2351 GCGCGAGAAG ATTGTGCACC GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT 2401 TCTACCATCG ACACCACCAC GCTGGCACCC AGTTGATCGG CGCGAGATTT 2451 AATCGCCGCG ACAATTTGCG ACGGCGCGTG CAGGGCCAGA CTGGAGGTGG 2501 CAACGCCAAT CAGCAACGAC TGTTTGCCCG CCAGTTGTTG TGCCACGCGG 2551 TTGGGAATGT AATTCAGCTC CGCCATCGCC GCTTCCACTT TTTCCCGCGT 2601 TTTCGCAGAA ACGTGGCTGG CCTGGTTCAC CACGCGGGAA ACGGTCTGAT 2651 AAGAGACACC GGCATACTCT GCGACATCGT ATAACGTTAC TGGTTTCACA 2701 TTCACCACCC TGAATTGACT CTCTTCCGGG CGCTATCATG CCATACCGCG 2751 AAAGGTTTTG CGCCATTCGA TGGTGTCCGG GATCTCGACG CTCTCCCTTA 2801 TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG 2851 CACCGCCGCC GCAAGGAATG GTGTCGTCGC CGCACTTATG ACTGTCTTCT 2901 TTATCATGCA ACTCGTAGGA CAGGTGCCGG CAGCGCCCAA CAGTCCCCCG 2951 GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC CCTGCACCAT 3001 TATGTTCCGG ATCTGCATCG CAGGATGCTG CTGGCTACCC TGTGGAACAC 3051 CTACATCTGT ATTAACGAAG CGCTAACCGT TTTTATCAGG CTCTGGGAGG 3101 CAGAATAAAT GATCATATCG TCAATTATTA CCTCCACGGG GAGAGCCTGA 3151 GCAAACTGGC CTCAGGCATT TGAGAAGCAC ACGGTCACAC TGCTTCCGGT 3201 AGTCAATAAA CCGGTAAACC AGCAATAGAC ATAAGCGGCT ATTTAACGAC 3251 CCTGCCCTGA ACCGACGACC GGGTCGAATT TGCTTTCGAA TTTCTGCCAT 3301 TCATCCGCTT ATTATCACTT ATTCAGGCGT AGCACCAGGC GTTTAAGGGC 3351 ACCAATAACT GCCTTAAAAA AATTACGCCC CGCCCTGCCA CTCATCGCAG 3401 TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAGAC 3451 GGCATGATGA ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG 3501 TATAATATTT GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG 3551 GCCACGTTTA AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC 3601 GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC 3651 CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG 3701 TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA 3751 AACGGTGTAA CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT 3801 TCATTGCCAT ACG
