pETcoco-2

At a Glance

Type	Expression
Origin of Replication	oriV, oriS
Copy #
Markers	Ampicillin
Link to Sequence	Novagen
Comments	The pETcoco™-2 expression vector is designed to allow "on command" amplification of vector copy number from single copy (1–2 plasmid copies per cell) to medium copy (20–50 per cell).
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History

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Sources

Novagen

References

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Sequence

       1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag 
      61 gagatataag catgcatcac catcaccatc acggtgctag caaagaaacc gctgctgcga 
     121 aatttgaacg ccagcacatg gactcgccac cgccaactgg tgatgacgac gacaagagcc 
     181 cgggcgatcg catcgatgac gtccaggcgc gccacgtgcg tacggtttcg aagcttgcgg 
     241 ccgcaagcca gccagaactc gctccggaag atcccgagga tctttaatta acctcaggat 
     301 ttaaataacc taggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa 
     361 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc 
     421 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg 
     481 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 
     541 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 
     601 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 
     661 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 
     721 tcacggccgc cgcaatgctt gcataatgtg gcctgtcaaa tggacgaagc agggattctg 
     781 caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa ttatgacaac 
     841 ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct cgggctggcc 
     901 ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc aacattgcga 
     961 ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg gctgatacgt 
    1021 tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga 
    1081 cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc 
    1141 aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg cggatggagc 
    1201 gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc 
    1261 agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg 
    1321 aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa tattgacggc 
    1381 cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat 
    1441 tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata 
    1501 tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg 
    1561 gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa 
    1621 ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga gtatcccggc 
    1681 agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt cagagaagaa 
    1741 accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg 
    1801 ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc 
    1861 atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat 
    1921 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct 
    1981 acctgacgct ttttatcgca actctctact gtttctccat acccgttttt ttggatggag 
    2041 tgaaaggagg aattcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg 
    2101 aaggcataca ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc 
    2161 gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc 
    2221 cagcaagcta cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc 
    2281 gcgccatcgg ccgccgtgga gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg 
    2341 aagtcgatga ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc 
    2401 gaggacctgg caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag 
    2461 cagcagatca aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg 
    2521 cgagcgatgc caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc 
    2581 ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc 
    2641 tacaccggcg tcgagctgcg ggccgacgat gacgaactgg tgtggctgca ggtgttggag 
    2701 tacgcgaagc gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag 
    2761 gacctgggct ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc 
    2821 ctacaggcga cggcgatgga cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg 
    2881 ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg 
    2941 atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag 
    3001 aagtaccgca agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg 
    3061 gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc 
    3121 gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg 
    3181 gtggaacacg cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg 
    3241 tcagttccgg ctgggggttc agcagccacc gggccgtcga ccaattctca tgtttgacag 
    3301 cttatcatcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca 
    3361 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg 
    3421 cagttgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
    3481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
    3541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagaa ccacgctcac 
    3601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
    3661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
    3721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
    3781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
    3841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
    3901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
    3961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
    4021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
    4081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
    4141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
    4201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
    4261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
    4321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
    4381 tttagaaaaa taaacaaata ggggtttacc gctgaaagtt ctgcaaagcc tgatgggaca 
    4441 taagtccatc agttcaacgg aagtctacac gaaggttttt gcgctggatg tggctgcccg 
    4501 gcaccgggtg cagtttgcga tgccggagtc tgatgcggtt gcgatgctga aacaattatc 
    4561 ctgagaataa atgccttggc ctttatatgg aaatgtggaa ctgagtggat atgctgtttt 
    4621 tgtctgttaa acagagaagc tggctgttat ccactgagaa gcgaacgaaa cagtcgggaa 
    4681 aatctcccat tatcgtagag atccgcatta ttaatctcag gagcctgtgt agcgtttata 
    4741 ggaagtagtg ttctgtcatg atgcctgcaa gcggtaacga aaacgatttg aatatgcctt 
    4801 caggaacaat agaaatcttc gtgcggtgtt acgttgaagt ggagcggatt atgtcagcaa 
    4861 tggacagaac aacctaatga acacagaacc atgatgtggt ctgtcctttt acagccagta 
    4921 gtgctcgccg cagtcgagcg acagggcgaa gccctcgaga tcccctgcca tttttggggt 
    4981 gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggc 
    5041 cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc 
    5101 gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg 
    5161 ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga 
    5221 cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt 
    5281 caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc 
    5341 acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc 
    5401 gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc 
    5461 tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc 
    5521 agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccggccg cggtgtctcg 
    5581 cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag 
    5641 cggcgagggc aaccagcccg gtgagcgtcg gaaaggggaa tttcgagtga gcgaggaagc 
    5701 accagggaac agcacttata tattctgctt acacacgatg cctgaaaaaa cttcccttgg 
    5761 ggttatccac ttatccacgg ggatattttt ataattattt tttttatagt ttttagatct 
    5821 tcttttttag agcgccttgt aggcctttat ccatgctggt tctagagaag gtgttgtgac 
    5881 aaattgccct ttcagtgtga caaatcaccc tcaaatgaca gtcctgtctg tgacaaattg 
    5941 cccttaaccc tgtgacaaat tgccctcaga agaagctgtt ttttcacaaa gttatccctg 
    6001 cttattgact cttttttatt tagtgtgaca atctaaaaac ttgtcacact tcacatggat 
    6061 ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg taaaaatagc ccgcgaatcg 
    6121 tccagtcaaa cgacctcact gaggcggcat atagtctctc ccgggatcaa aaacgtatgc 
    6181 tgtatctgtt cgttgaccag atcagaaaat ctgatggcac cctacaggaa catgacggta 
    6241 tctgcgagat ccatgttgct aaatatgctg aaatattcgg attgacctct gcggaagcca 
    6301 gtaaggatat acggcaggca ttgaagagtt tcgcggggaa ggaagtggtt ttttatcgcc 
    6361 ctgaagagga tgccggcgat gaaaaaggct atgaatcttt tccttggttt atcaaacgtg 
    6421 cgcacagtcc atccagaggg ctttacagtg tacatatcaa cccatatctc attcccttct 
    6481 ttatcgggtt acagaaccgg tttacgcagt ttcggcttag tgaaacaaaa gaaatcacca 
    6541 atccgtatgc catgcgttta tacgaatccc tgtgtcagta tcgtaagccg gatggctcag 
    6601 gcatcgtctc tctgaaaatc gactggatca tagagcgtta ccagctgcct caaagttacc 
    6661 agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg tgttaatgag atcaacagca 
    6721 gaactccaat gcgcctctca tacattgaga aaaagaaagg ccgccagacg actcatatcg 
    6781 tattttcctt ccgcgatatc acttccatga cgacaggata gtctgagggt tatctgtcac 
    6841 agatttgagg gtggttcgtc acatttgttc tgacctactg agggtaattt gtcacagttt 
    6901 tgctgtttcc ttcagcctgc atggattttc tcatactttt tgaactgtaa tttttaagga 
    6961 agccaaattt gagggcagtt tgtcacagtt gatttccttc tctttccctt cgtcatgtga 
    7021 cctgatatcg ggggttagtt cgtcatcatt gatgagggtt gattatcaca gtttattact 
    7081 ctgaattggc tatccgcgtg tgtacctcta cctggagttt ttcccacggt ggatatttct 
    7141 tcttgcgctg agcgtaagag ctatctgaca gaacagttct tctttgcttc ctcgccagtt 
    7201 cgctcgctat gctcggttac acggctgcgg cgagcgctag tgataataag tgactgaggt 
    7261 atgtgctctt cttatctcct tttgtagtgt tgctcttatt ttaaacaact ttgcggtttt 
    7321 ttgatgactt tgcgattttg ttgttgcttt gcagtaaatt gcaagattta ataaaaaaac 
    7381 gcaaagcaat gattaaagga tgttcagaat gaaactcatg gaaacactta accagtgcat 
    7441 aaacgctggt catgaaatga cgaaggctat cgccattgca cagtttaatg atgacagccc 
    7501 ggaagcgagg aaaataaccc ggcgctggag aataggtgaa gcagcggatt tagttggggt 
    7561 ttcttctcag gctatcagag atgccgagaa agcagggcga ctaccgcacc cggatatgga 
    7621 aattcgagga cgggttgagc aacgtgttgg ttatacaatt gaacaaatta atcatatgcg 
    7681 tgatgtgttt ggtacgcgat tgcgacgtgc tgaagacgta tttccaccgg tgatcggggt 
    7741 tgctgcccat aaaggtggcg tttacaaaac ctcagtttct gttcatcttg ctcaggatct 
    7801 ggctctgaag gggctacgtg ttttgctcgt ggaaggtaac gacccccagg gaacagcctc 
    7861 aatgtatcac ggatgggtac cagatcttca tattcatgca gaagacactc tcctgccttt 
    7921 ctatcttggg gaaaaggacg atgtcactta tgcaataaag cccacttgct ggccggggct 
    7981 tgacattatt ccttcctgtc tggctctgca ccgtattgaa actgagttaa tgggcaaatt 
    8041 tgatgaaggt aaactgccca ccgatccaca cctgatgctc cgactggcca ttgaaactgt 
    8101 tgctcatgac tatgatgtca tagttattga cagcgcgcct aacctgggta tcggcacgat 
    8161 taatgtcgta tgtgctgctg atgtgctgat tgttcccacg cctgctgagt tgtttgacta 
    8221 cacctccgca ctgcagtttt tcgatatgct tcgtgatctg ctcaagaacg ttgatcttaa 
    8281 agggttcgag cctgatgtac gtattttgct taccaaatac agcaatagta atggctctca 
    8341 gtccccgtgg atggaggagc aaattcggga tgcctgggga agcatggttc taaaaaatgt 
    8401 tgtacgtgaa acggatgaag ttggtaaagg tcagatccgg atgagaactg tttttgaaca 
    8461 ggccattgat caacgctctt caactggtgc ctggagaaat gctctttcta tttgggaacc 
    8521 tgtctgcaat gaaattttcg atcgtctgat taaaccacgc tgggagatta gataatgaag 
    8581 cgtgcgcctg ttattccaaa acatacgctc aatactcaac cggttgaaga tacttcgtta 
    8641 tcgacaccag ctgccccgat ggtggattcg ttaattgcgc gcgtaggagt aatggctcgc 
    8701 ggtaatgcca ttactttgcc tgtatgtggt cgggatgtga agtttactct tgaagtgctc 
    8761 cggggtgata gtgttgagaa gacctctcgg gtatggtcag gtaatgaacg tgaccaggag 
    8821 ctgcttactg aggacgcact ggatgatctc atcccttctt ttctactgac tggtcaacag 
    8881 acaccggcgt tcggtcgaag agtatctggt gtcatagaaa ttgccgatgg gagtcgccgt 
    8941 cgtaaagctg ctgcacttac cgaaagtgat tatcgtgttc tggttggcga gctggatgat 
    9001 gagcagatgg ctgcattatc cagattgggt aacgattatc gcccaacaag tgcttatgaa 
    9061 cgtggtcagc gttatgcaag ccgattgcag aatgaatttg ctggaaatat ttctgcgctg 
    9121 gctgatgcgg aaaatatttc acgtaagatt attacccgct gtatcaacac cgccaaattg 
    9181 cctaaatcag ttgttgctct tttttctcac cccggtgaac tatctgcccg gtcaggtgat 
    9241 gcacttcaaa aagcctttac agataaagag gaattactta agcagcaggc atctaacctt 
    9301 catgagcaga aaaaagctgg ggtgatattt gaagctgaag aagttatcac tcttttaact 
    9361 tctgtgctta aaacgtcatc tgcatcaaga actagtttaa gctcacgaca tcagtttgct 
    9421 cctggagcga cagtattgta taagggcgat aaaatggtgc ttaacctgga caggtctcgt 
    9481 gttccaactg agtgtataga gaaaattgag gccattctta aggaacttga aaagccagca 
    9541 ccctgatgcg accacgtttt agtctacgtt tatctgtctt tacttaatgt cctttgttac 
    9601 aggccagaaa gcataactgg cctgaatatt ctctctgggc ccactgttcc acttgtatcg 
    9661 tcggtctgat aatcagactg ggaccacggt cccactcgta tcgtcggtct gattattagt 
    9721 ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca 
    9781 ctcgtatcgt cggtctgata atcagactgg gaccacggtc ccactcgtat cgtcggtctg 
    9841 attattagtc tgggaccatg gtcccactcg tatcgtcggt ctgattatta gtctgggacc 
    9901 acggtcccac tcgtatcgtc ggtctgatta ttagtctgga accacggtcc cactcgtatc 
    9961 gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgattattag 
   10021 tctgggacca cgatcccact cgtgttgtcg gtctgattat cggtctggga ccacggtccc 
   10081 acttgtattg tcgatcagac tatcagcgtg agactacgat tccatcaatg cctgtcaagg 
   10141 gcaagtattg acatgtcgtc gtaacctgta gaacggagta acctcggtgt gcggttgtat 
   10201 gcctgctgtg gattgctgct gtgtcctgct tatccacaac attttgcgca cggttatgtg 
   10261 gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa ccgggctgca tccgatgcaa 
   10321 gtgtgtcgct gtcgacgagc tcgcgagctc ggacatgagg ttgccccgta ttcagtgtcg 
   10381 ctgatttgta ttgtctgaag ttgtttttac gttaagttga tgcagatcaa ttaatacgat 
   10441 acctgcgtca taattgatta tttgacgtgg tttgatggcc tccacgcacg ttgtgatatg 
   10501 tagatgataa tcattatcac tttacgggtc ctttccggtg atccgacagg ttacggggcg 
   10561 gcgacctcgc gggttttcgc tatttatgaa aattttccgg tttaaggcgt ttccgttctt 
   10621 cttcgtcata acttaatgtt tttatttaaa ataccctctg aaaagaaagg aaacgacagg 
   10681 tgctgaaagc gagctttttg gcctctgtcg tttcctttct ctgtttttgt ccgtggaatg 
   10741 aacaatggaa gtccgagctc atcgctaata acttcgtata gcatacatta tacgaagtta 
   10801 tattcgatgc ggcctagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc 
   10861 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc 
   10921 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 
   10981 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca 
   11041 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca 
   11101 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg 
   11161 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa 
   11221 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa 
   11281 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg 
   11341 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat 
   11401 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca 
   11461 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt 
   11521 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg 
   11581 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa 
   11641 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga 
   11701 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt 
   11761 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa 
   11821 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct 
   11881 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca 
   11941 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta 
   12001 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc 
   12061 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc 
   12121 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat 
   12181 ggtgcatgta aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc 
   12241 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg 
   12301 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt 
   12361 ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactata