pETcoco-2
At a Glance
pETcoco-2, Image made with PlasMapper
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication |
oriV, oriS |
| Copy # |
1-2 or 20-50 |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
T7 promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
The pETcoco™-2 allows for the "on command" amplification of vector copy number from single copy (1–2 plasmid copies per cell) to medium copy (20–50 per cell). pETcoco™-2 is maintained in the single copy state by using the oriS and repE elements of the F episome together with the parABC partition determinants. The medium copy state is obtained by employing the RK-2 derived trfA replicator acting at oriV. The switch from single copy to medium copy status is achieved by inducing the expression of the trfA gene with arabinose. The dual replicon nature of these vectors promotes enhanced stability of recombinant plasmids and extremely low basal expression levels in the single copy state, while allowing robust target gene expression typical of the pET vectors.
History
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Sources
References
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Sequence
1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
61 gagatataag catgcatcac catcaccatc acggtgctag caaagaaacc gctgctgcga
121 aatttgaacg ccagcacatg gactcgccac cgccaactgg tgatgacgac gacaagagcc
181 cgggcgatcg catcgatgac gtccaggcgc gccacgtgcg tacggtttcg aagcttgcgg
241 ccgcaagcca gccagaactc gctccggaag atcccgagga tctttaatta acctcaggat
301 ttaaataacc taggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
361 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
421 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
481 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
541 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
601 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
661 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
721 tcacggccgc cgcaatgctt gcataatgtg gcctgtcaaa tggacgaagc agggattctg
781 caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa ttatgacaac
841 ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct cgggctggcc
901 ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc aacattgcga
961 ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg gctgatacgt
1021 tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga
1081 cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc
1141 aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg cggatggagc
1201 gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc
1261 agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg
1321 aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa tattgacggc
1381 cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat
1441 tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata
1501 tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg
1561 gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa
1621 ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga gtatcccggc
1681 agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt cagagaagaa
1741 accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg
1801 ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc
1861 atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat
1921 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct
1981 acctgacgct ttttatcgca actctctact gtttctccat acccgttttt ttggatggag
2041 tgaaaggagg aattcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg
2101 aaggcataca ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc
2161 gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc
2221 cagcaagcta cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc
2281 gcgccatcgg ccgccgtgga gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg
2341 aagtcgatga ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc
2401 gaggacctgg caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag
2461 cagcagatca aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg
2521 cgagcgatgc caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc
2581 ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc
2641 tacaccggcg tcgagctgcg ggccgacgat gacgaactgg tgtggctgca ggtgttggag
2701 tacgcgaagc gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag
2761 gacctgggct ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc
2821 ctacaggcga cggcgatgga cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg
2881 ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg
2941 atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag
3001 aagtaccgca agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg
3061 gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc
3121 gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg
3181 gtggaacacg cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg
3241 tcagttccgg ctgggggttc agcagccacc gggccgtcga ccaattctca tgtttgacag
3301 cttatcatcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca
3361 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
3421 cagttgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
3481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
3541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagaa ccacgctcac
3601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
3661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
3721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
3781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
3841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
3901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
3961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
4021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
4081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
4141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
4201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
4261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
4321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
4381 tttagaaaaa taaacaaata ggggtttacc gctgaaagtt ctgcaaagcc tgatgggaca
4441 taagtccatc agttcaacgg aagtctacac gaaggttttt gcgctggatg tggctgcccg
4501 gcaccgggtg cagtttgcga tgccggagtc tgatgcggtt gcgatgctga aacaattatc
4561 ctgagaataa atgccttggc ctttatatgg aaatgtggaa ctgagtggat atgctgtttt
4621 tgtctgttaa acagagaagc tggctgttat ccactgagaa gcgaacgaaa cagtcgggaa
4681 aatctcccat tatcgtagag atccgcatta ttaatctcag gagcctgtgt agcgtttata
4741 ggaagtagtg ttctgtcatg atgcctgcaa gcggtaacga aaacgatttg aatatgcctt
4801 caggaacaat agaaatcttc gtgcggtgtt acgttgaagt ggagcggatt atgtcagcaa
4861 tggacagaac aacctaatga acacagaacc atgatgtggt ctgtcctttt acagccagta
4921 gtgctcgccg cagtcgagcg acagggcgaa gccctcgaga tcccctgcca tttttggggt
4981 gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggc
5041 cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc
5101 gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg
5161 ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga
5221 cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt
5281 caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc
5341 acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc
5401 gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc
5461 tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc
5521 agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccggccg cggtgtctcg
5581 cacacggctt cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag
5641 cggcgagggc aaccagcccg gtgagcgtcg gaaaggggaa tttcgagtga gcgaggaagc
5701 accagggaac agcacttata tattctgctt acacacgatg cctgaaaaaa cttcccttgg
5761 ggttatccac ttatccacgg ggatattttt ataattattt tttttatagt ttttagatct
5821 tcttttttag agcgccttgt aggcctttat ccatgctggt tctagagaag gtgttgtgac
5881 aaattgccct ttcagtgtga caaatcaccc tcaaatgaca gtcctgtctg tgacaaattg
5941 cccttaaccc tgtgacaaat tgccctcaga agaagctgtt ttttcacaaa gttatccctg
6001 cttattgact cttttttatt tagtgtgaca atctaaaaac ttgtcacact tcacatggat
6061 ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg taaaaatagc ccgcgaatcg
6121 tccagtcaaa cgacctcact gaggcggcat atagtctctc ccgggatcaa aaacgtatgc
6181 tgtatctgtt cgttgaccag atcagaaaat ctgatggcac cctacaggaa catgacggta
6241 tctgcgagat ccatgttgct aaatatgctg aaatattcgg attgacctct gcggaagcca
6301 gtaaggatat acggcaggca ttgaagagtt tcgcggggaa ggaagtggtt ttttatcgcc
6361 ctgaagagga tgccggcgat gaaaaaggct atgaatcttt tccttggttt atcaaacgtg
6421 cgcacagtcc atccagaggg ctttacagtg tacatatcaa cccatatctc attcccttct
6481 ttatcgggtt acagaaccgg tttacgcagt ttcggcttag tgaaacaaaa gaaatcacca
6541 atccgtatgc catgcgttta tacgaatccc tgtgtcagta tcgtaagccg gatggctcag
6601 gcatcgtctc tctgaaaatc gactggatca tagagcgtta ccagctgcct caaagttacc
6661 agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg tgttaatgag atcaacagca
6721 gaactccaat gcgcctctca tacattgaga aaaagaaagg ccgccagacg actcatatcg
6781 tattttcctt ccgcgatatc acttccatga cgacaggata gtctgagggt tatctgtcac
6841 agatttgagg gtggttcgtc acatttgttc tgacctactg agggtaattt gtcacagttt
6901 tgctgtttcc ttcagcctgc atggattttc tcatactttt tgaactgtaa tttttaagga
6961 agccaaattt gagggcagtt tgtcacagtt gatttccttc tctttccctt cgtcatgtga
7021 cctgatatcg ggggttagtt cgtcatcatt gatgagggtt gattatcaca gtttattact
7081 ctgaattggc tatccgcgtg tgtacctcta cctggagttt ttcccacggt ggatatttct
7141 tcttgcgctg agcgtaagag ctatctgaca gaacagttct tctttgcttc ctcgccagtt
7201 cgctcgctat gctcggttac acggctgcgg cgagcgctag tgataataag tgactgaggt
7261 atgtgctctt cttatctcct tttgtagtgt tgctcttatt ttaaacaact ttgcggtttt
7321 ttgatgactt tgcgattttg ttgttgcttt gcagtaaatt gcaagattta ataaaaaaac
7381 gcaaagcaat gattaaagga tgttcagaat gaaactcatg gaaacactta accagtgcat
7441 aaacgctggt catgaaatga cgaaggctat cgccattgca cagtttaatg atgacagccc
7501 ggaagcgagg aaaataaccc ggcgctggag aataggtgaa gcagcggatt tagttggggt
7561 ttcttctcag gctatcagag atgccgagaa agcagggcga ctaccgcacc cggatatgga
7621 aattcgagga cgggttgagc aacgtgttgg ttatacaatt gaacaaatta atcatatgcg
7681 tgatgtgttt ggtacgcgat tgcgacgtgc tgaagacgta tttccaccgg tgatcggggt
7741 tgctgcccat aaaggtggcg tttacaaaac ctcagtttct gttcatcttg ctcaggatct
7801 ggctctgaag gggctacgtg ttttgctcgt ggaaggtaac gacccccagg gaacagcctc
7861 aatgtatcac ggatgggtac cagatcttca tattcatgca gaagacactc tcctgccttt
7921 ctatcttggg gaaaaggacg atgtcactta tgcaataaag cccacttgct ggccggggct
7981 tgacattatt ccttcctgtc tggctctgca ccgtattgaa actgagttaa tgggcaaatt
8041 tgatgaaggt aaactgccca ccgatccaca cctgatgctc cgactggcca ttgaaactgt
8101 tgctcatgac tatgatgtca tagttattga cagcgcgcct aacctgggta tcggcacgat
8161 taatgtcgta tgtgctgctg atgtgctgat tgttcccacg cctgctgagt tgtttgacta
8221 cacctccgca ctgcagtttt tcgatatgct tcgtgatctg ctcaagaacg ttgatcttaa
8281 agggttcgag cctgatgtac gtattttgct taccaaatac agcaatagta atggctctca
8341 gtccccgtgg atggaggagc aaattcggga tgcctgggga agcatggttc taaaaaatgt
8401 tgtacgtgaa acggatgaag ttggtaaagg tcagatccgg atgagaactg tttttgaaca
8461 ggccattgat caacgctctt caactggtgc ctggagaaat gctctttcta tttgggaacc
8521 tgtctgcaat gaaattttcg atcgtctgat taaaccacgc tgggagatta gataatgaag
8581 cgtgcgcctg ttattccaaa acatacgctc aatactcaac cggttgaaga tacttcgtta
8641 tcgacaccag ctgccccgat ggtggattcg ttaattgcgc gcgtaggagt aatggctcgc
8701 ggtaatgcca ttactttgcc tgtatgtggt cgggatgtga agtttactct tgaagtgctc
8761 cggggtgata gtgttgagaa gacctctcgg gtatggtcag gtaatgaacg tgaccaggag
8821 ctgcttactg aggacgcact ggatgatctc atcccttctt ttctactgac tggtcaacag
8881 acaccggcgt tcggtcgaag agtatctggt gtcatagaaa ttgccgatgg gagtcgccgt
8941 cgtaaagctg ctgcacttac cgaaagtgat tatcgtgttc tggttggcga gctggatgat
9001 gagcagatgg ctgcattatc cagattgggt aacgattatc gcccaacaag tgcttatgaa
9061 cgtggtcagc gttatgcaag ccgattgcag aatgaatttg ctggaaatat ttctgcgctg
9121 gctgatgcgg aaaatatttc acgtaagatt attacccgct gtatcaacac cgccaaattg
9181 cctaaatcag ttgttgctct tttttctcac cccggtgaac tatctgcccg gtcaggtgat
9241 gcacttcaaa aagcctttac agataaagag gaattactta agcagcaggc atctaacctt
9301 catgagcaga aaaaagctgg ggtgatattt gaagctgaag aagttatcac tcttttaact
9361 tctgtgctta aaacgtcatc tgcatcaaga actagtttaa gctcacgaca tcagtttgct
9421 cctggagcga cagtattgta taagggcgat aaaatggtgc ttaacctgga caggtctcgt
9481 gttccaactg agtgtataga gaaaattgag gccattctta aggaacttga aaagccagca
9541 ccctgatgcg accacgtttt agtctacgtt tatctgtctt tacttaatgt cctttgttac
9601 aggccagaaa gcataactgg cctgaatatt ctctctgggc ccactgttcc acttgtatcg
9661 tcggtctgat aatcagactg ggaccacggt cccactcgta tcgtcggtct gattattagt
9721 ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca
9781 ctcgtatcgt cggtctgata atcagactgg gaccacggtc ccactcgtat cgtcggtctg
9841 attattagtc tgggaccatg gtcccactcg tatcgtcggt ctgattatta gtctgggacc
9901 acggtcccac tcgtatcgtc ggtctgatta ttagtctgga accacggtcc cactcgtatc
9961 gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgattattag
10021 tctgggacca cgatcccact cgtgttgtcg gtctgattat cggtctggga ccacggtccc
10081 acttgtattg tcgatcagac tatcagcgtg agactacgat tccatcaatg cctgtcaagg
10141 gcaagtattg acatgtcgtc gtaacctgta gaacggagta acctcggtgt gcggttgtat
10201 gcctgctgtg gattgctgct gtgtcctgct tatccacaac attttgcgca cggttatgtg
10261 gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa ccgggctgca tccgatgcaa
10321 gtgtgtcgct gtcgacgagc tcgcgagctc ggacatgagg ttgccccgta ttcagtgtcg
10381 ctgatttgta ttgtctgaag ttgtttttac gttaagttga tgcagatcaa ttaatacgat
10441 acctgcgtca taattgatta tttgacgtgg tttgatggcc tccacgcacg ttgtgatatg
10501 tagatgataa tcattatcac tttacgggtc ctttccggtg atccgacagg ttacggggcg
10561 gcgacctcgc gggttttcgc tatttatgaa aattttccgg tttaaggcgt ttccgttctt
10621 cttcgtcata acttaatgtt tttatttaaa ataccctctg aaaagaaagg aaacgacagg
10681 tgctgaaagc gagctttttg gcctctgtcg tttcctttct ctgtttttgt ccgtggaatg
10741 aacaatggaa gtccgagctc atcgctaata acttcgtata gcatacatta tacgaagtta
10801 tattcgatgc ggcctagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
10861 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
10921 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
10981 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
11041 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
11101 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
11161 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
11221 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
11281 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
11341 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
11401 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
11461 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
11521 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
11581 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
11641 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
11701 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
11761 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
11821 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
11881 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
11941 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
12001 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
12061 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
12121 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
12181 ggtgcatgta aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
12241 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
12301 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
12361 ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactata
