pMAL-pIII: Difference between revisions
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==History | ==History== | ||
The pMal-pIII Vector is a derivative of pMal-p2 in which the leader sequence of maltose binding protein (MBP, malE) has been replaced with the M13 pIII leader sequence. KpnI/Acc65I and EagI sites have been introduced within the pIII leader to facilitate direct transfer of sequences selected from any of the Ph.D. phage display peptide libraries into an expression vector. The corresponding peptides are expressed as N-terminal MBP fusions. These fusions can then be easily purified from E. coli periplasmic space by osmotic shock followed by affinity chromatography on amylose resin. Since the peptide is expressed at the N-terminus of MBP, Factor Xa cannot be used to cleave the peptide from MBP. | |||
==Sources {{HelpIcon|Help:Plasmid}}== | ==Sources {{HelpIcon|Help:Plasmid}}== | ||
Revision as of 17:24, 8 September 2007
At a Glance
| Type |
Cloning |
|---|---|
| Origin of Replication | |
| Copy # | |
| Markers | |
| Link to Sequence | |
| Comments | |
| edit table | |
History
The pMal-pIII Vector is a derivative of pMal-p2 in which the leader sequence of maltose binding protein (MBP, malE) has been replaced with the M13 pIII leader sequence. KpnI/Acc65I and EagI sites have been introduced within the pIII leader to facilitate direct transfer of sequences selected from any of the Ph.D. phage display peptide libraries into an expression vector. The corresponding peptides are expressed as N-terminal MBP fusions. These fusions can then be easily purified from E. coli periplasmic space by osmotic shock followed by affinity chromatography on amylose resin. Since the peptide is expressed at the N-terminus of MBP, Factor Xa cannot be used to cleave the peptide from MBP.
Sources
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References
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Sequence
You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.