pMAL-pIII
At a Glance
pMAL-pIII Image provided by NEB
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
M13, pMB1 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
lacIq Promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
Isolated from E. coli strain ER2272 by standard DNA purification procedure. Rapid characterization of selected sequences by ELISA Alternative to chemical peptide synthesis Monovalency of MBP fusion allows accurate KD determination.
History
pMal-pIII is a derivative of pMal-p2. Kpn1/Acc651 and EagI sites have been introduced within the pIII leader to ease direct transfer of sequences selected from any of the PhD phage display peptide libraries into an expression vector.
Sources
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC
51 CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA
101 CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG
151 TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG
201 GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC
251 GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC
301 ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG
351 GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG
401 TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA
451 TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC
501 ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA
551 CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC
601 TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT
651 TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG
701 CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT
751 CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT
801 GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT
851 TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG
901 ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA
951 CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT
1001 CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG
1051 TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC
1101 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA
1151 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG
1201 GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT
1251 GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC
1301 GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT
1351 AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG
1401 AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG
1451 GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA
1501 GCACTTCACC AACAAGGACC ATAGCATATG AAAAAATTAT TATTCGCAAT
1551 TCCTTTAGTG GTACCTTTCT ATTCTCACTC GGCCGATATG AAAATCGAAG
1601 AAGGTAAACT GGTAATCTGG ATTAACGGCG ATAAAGGCTA TAACGGTCTC
1651 GCTGAAGTCG GTAAGAAATT CGAGAAAGAT ACCGGAATTA AAGTCACCGT
1701 TGAGCATCCG GATAAACTGG AAGAGAAATT CCCACAGGTT GCGGCAACTG
1751 GCGATGGCCC TGACATTATC TTCTGGGCAC ACGACCGCTT TGGTGGCTAC
1801 GCTCAATCTG GCCTGTTGGC TGAAATCACC CCGGACAAAG CGTTCCAGGA
1851 CAAGCTGTAT CCGTTTACCT GGGATGCCGT ACGTTACAAC GGCAAGCTGA
1901 TTGCTTACCC GATCGCTGTT GAAGCGTTAT CGCTGATTTA TAACAAAGAT
1951 CTGCTGCCGA ACCCGCCAAA AACCTGGGAA GAGATCCCGG CGCTGGATAA
2001 AGAACTGAAA GCGAAAGGTA AGAGCGCGCT GATGTTCAAC CTGCAAGAAC
2051 CGTACTTCAC CTGGCCGCTG ATTGCTGCTG ACGGGGGTTA TGCGTTCAAG
2101 TATGAAAACG GCAAGTACGA CATTAAAGAC GTGGGCGTGG ATAACGCTGG
2151 CGCGAAAGCG GGTCTGACCT TCCTGGTTGA CCTGATTAAA AACAAACACA
2201 TGAATGCAGA CACCGATTAC TCCATCGCAG AAGCTGCCTT TAATAAAGGC
2251 GAAACAGCGA TGACCATCAA CGGCCCGTGG GCATGGTCCA ACATCGACAC
2301 CAGCAAAGTG AATTATGGTG TAACGGTACT GCCGACCTTC AAGGGTCAAC
2351 CATCCAAACC GTTCGTTGGC GTGCTGAGCG CAGGTATTAA CGCCGCCAGT
2401 CCGAACAAAG AGCTGGCAAA AGAGTTCCTC GAAAACTATC TGCTGACTGA
2451 TGAAGGTCTG GAAGCGGTTA ATAAAGACAA ACCGCTGGGT GCCGTAGCGC
2501 TGAAGTCTTA CGAGGAAGAG TTGGCGAAAG ATCCACGTAT TGCCGCCACT
2551 ATGGAAAACG CCCAGAAAGG TGAAATCATG CCGAACATCC CGCAGATGTC
2601 CGCTTTCTGG TATGCCGTGC GTACTGCGGT GATCAACGCC GCCAGCGGTC
2651 GTCAGACTGT CGATGAAGCC CTGAAAGACG CGCAGACTAA TTCGAGCTCG
2701 AACAACAACA ACAATAACAA TAACAACAAC CTCGGGATCG AGGGAAGGAT
2751 TTCAGAATTC GGATCCTCTA GAGTCGACCT GCAGGCAAGC TTGGCACTGG
2801 CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT
2851 AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA
2901 GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT
2951 GGCAGCTTGG CTGTTTTGGC GGATGAGATA AGATTTTCAG CCTGATACAG
3001 ATTAAATCAG AACGCAGAAG CGGTCTGATA AAACAGAATT TGCCTGGCGG
3051 CAGTAGCGCG GTGGTCCCAC CTGACCCCAT GCCGAACTCA GAAGTGAAAC
3101 GCCGTAGCGC CGATGGTAGT GTGGGGTCTC CCCATGCGAG AGTAGGGAAC
3151 TGCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC
3201 GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG
3251 CCGGGAGCGG ATTTGAACGT TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC
3301 AGGACGCCCG CCATAAACTG CCAGGCATCA AATTAAGCAG AAGGCCATCC
3351 TGACGGATGG CCTTTTTGCG TTTCTACAAA CTCTTTTTGT TTATTTTTCT
3401 AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG
3451 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT
3501 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC
3551 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA
3601 GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT
3651 TCGCCCCGAA GAACGTTCTC CAATGATGAG CACTTTTAAA GTTCTGCTAT
3701 GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC
3751 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA
3801 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA
3851 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA
3901 GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC
3951 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG
4001 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA
4051 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG
4101 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG
4151 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC
4201 GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT
4251 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA
4301 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA
4351 GTTTACTCAT ATATACTTTA GATTGATTTA CCCCGGTTGA TAATCAGAAA
4401 AGCCCCAAAA ACAGGAAGAT TGTATAAGCA AATATTTAAA TTGTAAACGT
4451 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT
4501 TTAACCAATA GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG
4551 ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT
4601 AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG
4651 ATGGCCCACT ACGTGAACCA TCACCCAAAT CAAGTTTTTT GGGGTCGAGG
4701 TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
4751 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA
4801 AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
4851 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTAAAAGGA
4901 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT
4951 GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
5001 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
5051 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT
5101 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
5151 CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
5201 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
5251 GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
5301 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
5351 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA
5401 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
5451 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
5501 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
5551 CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
5601 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
5651 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC
5701 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
5751 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA
5801 CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA
5851 TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC
5901 GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG
5951 CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC
6001 CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC
6051 GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG CAGCGATTCA
6101 CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG
6151 CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT
6201 CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG
6251 GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA
6301 TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG
6351 TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC
6401 TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC
6451 GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA
6501 GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC
6551 GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA
6601 TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA
6651 ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC
6701 GAAATT
