Addgene: Difference between revisions
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Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request. | Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request. | ||
==Content{{HelpIcon|Help:Databases}}== | |||
'''Plasmid Repository''' | |||
In 2014 Addgene's repository distributes 30,000 types of plasmids, deposited by 1,700 labs.<ref>{{cite web|last=Baker|first=Monya|title=Repositories share key research tools|url=http://www.nature.com/news/repositories-share-key-research-tools-1.14511|publisher=Nature.com|accessdate=8 May 2014}}</ref> | |||
Addgene’s plasmid collection contains plasmids used for a variety of functions such as [http://www.addgene.org/Genome_Engineering/ genome engineering] (including CRISPRS), gene expression, shRNA knockdown, viral-mediated gene delivery, detection of miRNA and promoter activity. | |||
The plasmid collection includes: | |||
* Genome engineering | |||
** [http://www.addgene.org/CRISPR/ CRISPR]s | |||
** [http://www.addgene.org/TALEN/guide/ Transcription Activator-Like Effector Nuclease] (TALEN) kits | |||
** [http://www.addgene.org/zfc/ Zinc finger nuclease] kits | |||
* Empty backbones | |||
** Species-specific expression | |||
** Epitope tags | |||
** Fusion proteins | |||
** Selectable markers | |||
** [http://www.addgene.org/fluorescent_proteins/ Fluorescent marker] | |||
* Viral vectors | |||
** [http://www.addgene.org/lentiviral/ Retroviral/Lentiviral] | |||
** Adenoviral | |||
** Adeno-associated virus | |||
* cDNA expression | |||
* shRNA expression | |||
==Tools and guides== | |||
'''Molecular Biology Tools''' | |||
[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions. | |||
[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites. | |||
[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations. | |||
'''Plasmid Cloning Guides''' | |||
[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning. | |||
[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation. | |||
==Content {{HelpIcon|Help:Databases}}== | ==Content {{HelpIcon|Help:Databases}}== | ||
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==References {{HelpIcon|Help:Databases}}== | ==References {{HelpIcon|Help:Databases}}== | ||
Revision as of 13:57, 2 July 2014
You can help EcoliWiki by editing the content of this page. For information about becoming a registered user and obtaining editing privileges, see Help:Accounts.
| Link/URL: | ||
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| What: |
Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) Click to search vector database now. |
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| Who: |
Addgene is a non-profit global plasmid repository dedicated to helping scientists share their plasmids. |
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| Updates: |
Do you use plasmids? Join the non-profit plasmid sharing community. Visit www.addgene.org to learn more, find plasmids, and start depositing! |
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| Upcoming events: |
Visit Addgene's News and Announcements page to see current news and announcements. |
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| Web Services: |
Addgene is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world. |
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| edit table |
See Help:Database_table for how to add or edit information in this section of EcoliWiki.
About Addgene
Addgene is a non-profit organization dedicated to making it easier for scientists to share plasmids.
Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.
Content
Plasmid Repository
In 2014 Addgene's repository distributes 30,000 types of plasmids, deposited by 1,700 labs.[1] Addgene’s plasmid collection contains plasmids used for a variety of functions such as genome engineering (including CRISPRS), gene expression, shRNA knockdown, viral-mediated gene delivery, detection of miRNA and promoter activity.
The plasmid collection includes:
- Genome engineering
- CRISPRs
- Transcription Activator-Like Effector Nuclease (TALEN) kits
- Zinc finger nuclease kits
- Empty backbones
- Species-specific expression
- Epitope tags
- Fusion proteins
- Selectable markers
- Fluorescent marker
- Viral vectors
- Retroviral/Lentiviral
- Adenoviral
- Adeno-associated virus
- cDNA expression
- shRNA expression
Tools and guides
Molecular Biology Tools
Vector Database- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.
Sequence Analyzer- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.
Molecular Biology Reference- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.
Plasmid Cloning Guides
Molecular Cloning Guides- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.
Molecular Cloning Protocols- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.
Content
Addgene is a not-for-profit plasmid repository with an online database of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. Only the plasmids deposited by scientists at Addgene are available for purchase through this website.
Using Addgene
Browsing
Browse Plasmid Repository by plasmid type, source, bacterial resistance, and selectable marker.
| Browse By Plasmid Type: | |
|---|---|
| edit table |
Searching
| Additional Searches By: | |
|---|---|
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Bacterial Resistance |
Ampicillin; Kanamycin; Tetracycline; Chloramphenicol; Hygromycin; Spectinomycin; Gentamycin |
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Selectable Marker |
Neomycin; Puromycin; Hygromycin; Zeocin; URA3; LEU2; TRP1;HIS3 |
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Source |
Addgene; ATCC; BD Biosciences; Invitrogen; Novagen; Sigma; Stratagene |
| edit table |
References
www.addgene.org Addgene's Vector Database
External Links
[link Addgene] URL:link