pBAD24: Difference between revisions
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Table edited by Siegele via TableEdit |
Table edited by Siegele via TableEdit |
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| Line 38: | Line 38: | ||
*''araC'' | *''araC'' | ||
*optimized SD sequence | *optimized SD sequence | ||
*Kozak sequence (for efficient translation in eukaryotic cells)<ref name='PMID:3313277'/> | |||
*translation start codon | *translation start codon | ||
|- | |- | ||
!align=left {{table heading style}} |Accession Number | !align=left {{table heading style}} |Accession Number | ||
Revision as of 00:25, 8 July 2011
See Help:Plasmid for more information about plasmids in EcoliWiki.
At a Glance
| Type |
Cloning Expression Vector |
|---|---|
| Origin of Replication |
|
| Copy # | |
| Markers |
AmpR |
| Link to Sequence | |
| Features |
|
| Accession Number | |
| edit table |
Notes
- used to clone gene which lack sequences for initiation of translation[2]
History
Construction described in Guzman et al. (1995)[2].
Sources
You can help EcoliWiki by writing about the available sources of this plasmid.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Kozak, M (1987) An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 15 8125-48 PubMed
- ↑ 2.0 2.1 Guzman, LM et al. (1995) Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177 4121-30 PubMed
Sequence
You can help EcoliWiki by providing database accessions or, if none are available, the sequence of this plasmid.