mqsA:Quickview: Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
← Older edit
Twoodengr (talk | contribs)
64 edits
touch
 
(16 intermediate revisions by 2 users not shown)
Line 10: Line 10:
-->{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
-->{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}   
<noinclude>{{TabsTop}}   
{{Tab On|Quickview|ygiT}}{{Tab|Gene|ygiT}}{{Tab|Gene Product(s)|ygiT}}{{Tab|Expression|ygiT}}{{Tab|Evolution|ygiT}}{{Tab|On One Page|ygiT}}{{TabsBottom}}
{{Tab On|Quickview|mqsA}}{{Tab|Gene|mqsA}}{{Tab|Gene Product(s)|mqsA}}{{Tab|Expression|mqsA}}{{Tab|Evolution|mqsA}}{{Tab|On One Page|mqsA}}{{TabsBottom}}
{|
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"  
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"  
Line 23: Line 23:
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*  
*  
****************************************************************************************** -->{{Quickview_table_help}}
****************************************************************************************** -->
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  
{|   id="P49b0312855e29" class=" tableEdit New_Quickview_Table"


|-
|-
!align=left {{table_heading_style}} |Standard Name
!align=left {{table_heading_style}} |Standard Name
||
||
''ygiT''  
''mqsA''
|-
|-
!align=left {{table_heading_style}} |Gene Synonym(s)
!align=left {{table_heading_style}} |Gene Synonym(s)
||
||
''ECK3012, b3021, JW2989''<ref name='LIB:Riley_2006'/>
''mqsA, ygiT<ref name='PMID:20041169'/>, ECK3012, b3021, mqsA, JW2989''<ref name='LIB:Riley_2006'/>, JW2989, ygiT''
|-
|-
!align=left {{table_heading_style}} |Product Desc.
!align=left {{table_heading_style}} |Product Desc.
Line 39: Line 39:
predicted DNA-binding transcriptional regulator<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>
predicted DNA-binding transcriptional regulator<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>


Antitoxin for MqsR toxin<ref name='LIB:EcoGene'/>
Antitoxin for MqsR toxin<ref name='PMID:20041169'/>
|-
|-
!align=left {{table_heading_style}} |Product Synonyms(s)
!align=left {{table_heading_style}} |Product Synonyms(s)
||
||
predicted DNA-binding transcriptional regulator<ref name='LIB:Riley_2006'/>, B3021<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YgiT<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''predicted DNA-binding transcriptional regulator<ref name='LIB:Riley_2006'/>, B3021<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, MqsA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK3012, JW2989, ygiT, b3021''
|-
!align=left {{table_heading_style}} |Function from GO
||
<GO_nr />
|-
|-
!align=left {{table_heading_style}} |Knock-Out Phenotype
!align=left {{table_heading_style}} |Knock-Out Phenotype
Line 51: Line 55:
!align=left {{table_heading_style}} |Regulation/Expression
!align=left {{table_heading_style}} |Regulation/Expression
||
||
transcription unit(s): [[:Category:Txn_unit:ygiT|ygiT]]<ref name='LIB:EcoCyc10.6'/>  
transcription unit(s): [[:Category:Txn_unit:mqsA|mqsA]]<ref name='LIB:EcoCyc10.6'/>  
|-
|-
!align=left {{table_heading_style}} |Regulation/Activity
!align=left {{table_heading_style}} |Regulation/Activity
||
||


|-
!align=left {{table_heading_style}} |Quick Links
||
[[File:porteco.png|link=http://porteco.org/AjaxSearch.jsp?searchString=ygiT&s=search]] <br/><br/> [[File:dnadisplay.png|link=http://ecoliwiki.net/tools/display_dna.php?gene=ygiT&loc=MG1655%3A3166268..3165873,W3110%3A3166902..3166507,DH10B%3A3264013..3263618,REL606%3A3102418..3102023,BW2952%3A3053021..3053416]] [[File:proteindisplay.png|link=http://ecoliwiki.net/tools/display_protein.php?f=NC_000913&start=3053021&end=3053416&gene=ygiT&aa=mkcpvchqgemvsgikdipytfrgrktvlkgihglycvhceesimnkeesdafmaqvkafrasvnaetvapefivkvrkklsltqkeaseifgggvnafsryekgnaqphpstikllrvldkhpellneir]]&nbsp;&nbsp;  [[File:pubmed.jpg|link=http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=Search&Term=ygiT+AND+escherichia+coli]]&nbsp;&nbsp;  [[File:textpresso.jpg|link=http://textpresso.tamu.edu/cgi-bin/textpresso/search?q=ygiT]]&nbsp;&nbsp;


|-class='sortbottom'
|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.7786.P49b0312855e29&page=7786&pagename={{FULLPAGENAMEE}}&type=1&template=New_Quickview_Table edit table] ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.7786.P49b0312855e29&page=7786&pagename={{FULLPAGENAMEE}}&type=1&template=New_Quickview_Table edit table]</span> ||
|}
|}
<!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.7786.P49b0312855e29--></protect>
<!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.7786.P49b0312855e29--></protect>
Line 66: Line 74:
{{BacktoTop}}
{{BacktoTop}}


Antitoxin MqsA binds DNA via its helix-turn-helix in its C-terminal domain<ref name='PMID:20041169'/>.  Unlike most TA pairs including RelE/RelB, YoeB/YefM and MazF/MazE, the MqsR toxin gene precedes the antitoxin gene in the bicistronic operon<ref name='PMID:20041169'/>.  Furthermore, the MqsR/MqsA TA pair is unique in that (i) the antitoxin is larger than the toxin, (ii) both toxin and antitoxin are basic (typically the toxin is basic while the antitoxin is acidic), (iii) the mqsRA sequences are not homologous to any member of a recognized TA system, (iv) the antitoxin binds the toxin at its amino terminus and requires a metal, zinc, for structural stability, (v) the antitoxin is structured throughout its entire sequence, and (vi) the antitoxin binds more than its own promoter (e.g., ''mqsRA'', ''mcbR'', and ''spy'') via its C-terminal domain<ref name='PMID:20041169'/> which implies it regulates more than its own transcription.  MqsR cleaves mRNA at GCU sites <ref name='PMID:19690171'/>.  Toxin MsqR could be cloned in an ara vector unless antitoxin MqsA is upreguated (Shah, 2006). MqsA is predicted to also be a transcriptional regulator.<ref name='LIB:EcoGene'/>
Antitoxin MqsA binds DNA via its helix-turn-helix in its C-terminal domain<ref name='PMID:20041169'/>.  Unlike most TA pairs including RelE/RelB, YoeB/YefM and MazF/MazE, the MqsR toxin gene precedes the antitoxin gene in the bicistronic operon<ref name='PMID:20041169'/>.  The MqsR/MqsA TA pair is unique in that (i) the antitoxin is larger than the toxin, (ii) both toxin and antitoxin are basic (typically the toxin is basic while the antitoxin is acidic), (iii) the mqsRA sequences are not homologous to any member of a recognized TA system, (iv) the antitoxin binds the toxin at its amino terminus and requires a metal, zinc, for structural stability, (v) the antitoxin is structured throughout its entire sequence, and (vi) the antitoxin binds more than its own promoter (e.g., ''mqsRA'', ''mcbR'', and ''spy'') via its C-terminal domain<ref name='PMID:20041169'/> which implies it regulates more than its own transcription.  MqsR cleaves mRNA at GCU sites <ref name='PMID:19690171'/>.  Toxin MsqR could be cloned in an ara vector unless antitoxin MqsA is upreguated (Shah, 2006). MqsA is predicted to also be a transcriptional regulator.<ref name='LIB:EcoGene'/>




Latest revision as of 21:41, 27 May 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
References Suggestions
Standard Name

mqsA

Gene Synonym(s)

mqsA, ygiT[1], ECK3012, b3021, mqsA, JW2989[2], JW2989, ygiT

Product Desc.

predicted DNA-binding transcriptional regulator[3][4]

Antitoxin for MqsR toxin[1]

Product Synonyms(s)

predicted DNA-binding transcriptional regulator[2], B3021[3][2], MqsA[3][2] , ECK3012, JW2989, ygiT, b3021

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): mqsA[3]

Regulation/Activity
Quick Links



        

edit table

See Help:Quickview for help with entering information in the Quickview table.

Notes

[back to top]


Antitoxin MqsA binds DNA via its helix-turn-helix in its C-terminal domain[1]. Unlike most TA pairs including RelE/RelB, YoeB/YefM and MazF/MazE, the MqsR toxin gene precedes the antitoxin gene in the bicistronic operon[1]. The MqsR/MqsA TA pair is unique in that (i) the antitoxin is larger than the toxin, (ii) both toxin and antitoxin are basic (typically the toxin is basic while the antitoxin is acidic), (iii) the mqsRA sequences are not homologous to any member of a recognized TA system, (iv) the antitoxin binds the toxin at its amino terminus and requires a metal, zinc, for structural stability, (v) the antitoxin is structured throughout its entire sequence, and (vi) the antitoxin binds more than its own promoter (e.g., mqsRA, mcbR, and spy) via its C-terminal domain[1] which implies it regulates more than its own transcription. MqsR cleaves mRNA at GCU sites [5]. Toxin MsqR could be cloned in an ara vector unless antitoxin MqsA is upreguated (Shah, 2006). MqsA is predicted to also be a transcriptional regulator.[6]



References

[back to top]


  1. ↑ 1.0 1.1 1.2 1.3 1.4 Brown, BL et al. (2009) Three dimensional structure of the MqsR:MqsA complex: a novel TA pair comprised of a toxin homologous to RelE and an antitoxin with unique properties. PLoS Pathog. 5 e1000706 PubMed
  2. ↑ 2.0 2.1 2.2 2.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  5. ↑ Yamaguchi, Y et al. (2009) MqsR, a crucial regulator for quorum sensing and biofilm formation, is a GCU-specific mRNA interferase in Escherichia coli. J. Biol. Chem. 284 28746-53 PubMed
  6. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=mqsA:Quickview&oldid=1394588"