PMID:10074094: Difference between revisions

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<span style = 'font-size: large'> '''Heath, RJ and Rock, CO'''  (1999) A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant.''J. Bacteriol.'' '''181''':1944-6</span>
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'''Heath, RJ and Rock, CO'''  (1999) A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant. ''J. Bacteriol.'' '''181''':1944-6
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!align=left  |Abstract
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The sn-glycerol-3-phosphate acyltransferase (plsB) catalyzes the first step in membrane phospholipid formation. A conditional Escherichia coli mutant (plsB26) has a single missense mutation (G1045A) predicting the expression of an acyltransferase with an Ala349Thr substitution. The PlsB26 protein had a significantly reduced glycerol-3-phosphate acyltransferase specific activity coupled with an elevated Km for glycerol-3-phosphate.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10074094 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93600 PMC93600]


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|{{TableHeadingColor}} |'''Abstract''' || The sn-glycerol-3-phosphate acyltransferase (plsB) catalyzes the first step in membrane phospholipid formation. A conditional Escherichia coli mutant (plsB26) has a single missense mutation (G1045A) predicting the expression of an acyltransferase with an Ala349Thr substitution. The PlsB26 protein had a significantly reduced glycerol-3-phosphate acyltransferase specific activity coupled with an elevated Km for glycerol-3-phosphate.
!align=left  |Keywords
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Alleles; Base Sequence; DNA Primers/genetics; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression; Genes, Bacterial; Glycerol-3-Phosphate O-Acyltransferase/genetics; Glycerol-3-Phosphate O-Acyltransferase/metabolism; Kinetics; Molecular Sequence Data; Mutation, Missense
|{{TableHeadingColor}} nowrap |'''Links''' ||  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10074094 PubMed]
 
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==Annotations {{HelpIcon|Help:Literature}}==
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
==EcoliWiki Links {{HelpIcon|Help:Literature}} ==

Latest revision as of 04:09, 15 September 2013

Citation

Heath, RJ and Rock, CO (1999) A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant. J. Bacteriol. 181:1944-6

Abstract

The sn-glycerol-3-phosphate acyltransferase (plsB) catalyzes the first step in membrane phospholipid formation. A conditional Escherichia coli mutant (plsB26) has a single missense mutation (G1045A) predicting the expression of an acyltransferase with an Ala349Thr substitution. The PlsB26 protein had a significantly reduced glycerol-3-phosphate acyltransferase specific activity coupled with an elevated Km for glycerol-3-phosphate.

Links

PubMed PMC93600

Keywords

Alleles; Base Sequence; DNA Primers/genetics; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression; Genes, Bacterial; Glycerol-3-Phosphate O-Acyltransferase/genetics; Glycerol-3-Phosphate O-Acyltransferase/metabolism; Kinetics; Molecular Sequence Data; Mutation, Missense

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