dsrA:Expression: Difference between revisions

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===Overview===
===Overview===
{{Expression_overview_placeholder}}
{{Expression_overview_placeholder}}
==Cellular Levels==
{{BacktoTop}}
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===Notes===
==Transcription and Transcriptional Regulation==
==Transcription and Transcriptional Regulation==
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[[:Category:Txn_unit:dsrA|dsrA]]  
[[:Category:Txn_unit:dsrA|dsrA]]  


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===Notes===
===Notes===
{{Expression_transcription_placeholder}}
{{Expression_transcription_placeholder}}
dsrAp is regulated by temperature and operates efficiently at 25 C but poorly at 42 C. <ref name='PMID:14594834'/>


==Translation and Regulation of Translation==
==Translation and Regulation of Translation==
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<protect>{{GBrowseFigureByCoord|2023317|2023357|ecoli|MG1655|Genes_unlabeled+DNA_unlabeled+flip|400|{{Nterm_closeup}} }}
<protect><gbrowseImage>
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===Notes===
===Notes===
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!|Allele Name!!Mutation!!Phenotype!!Reference
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dsrAp construct Δ37
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G to A at -8 relative to the transcription start site
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temperature regulation abolished
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PMID:14594834


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microarray  
microarray
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[http://genexpdb.ou.edu/index.php?query=b1954 GenExpDB:b1954] ([[EcoliGenExpDB|EcoliWiki Page]])  
[http://genexpdb.ou.edu/index.php?query=b1954 GenExpDB:b1954] ([[EcoliGenExpDB|EcoliWiki Page]])  
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Summary of data for dsrA from multiple microarray studies   
Summary of data for dsrA from multiple microarray studies   


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GFP fusion described in Zaslaver, <i>et al.</i><br />Plate:109-AZ19; Well:B4<ref name="PMID: 16862137" />  
GFP fusion described in Zaslaver, <i>et al.</i><br />Plate:109-AZ19; Well:B4<ref name="PMID: 16862137" />  


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!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G7047 EcoCyc:G7047]
EcoCyc:G7047
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EchoBASE]]
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB4281 EchoBASE:EB4281]
EchoBASE:EB4281
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EcoGene]]
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EcoGene:EG31118
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[http://ecogene.org/geneinfo.php?eg_id=EG31118 EcoGene:EG31118]
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[[RegulonDB]]
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RegulonDB:ECK120003633
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0006490
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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==References==
==References==
{{BacktoTop}}
{{BacktoTop}}
 
{{RefHelp}}
<references/>
<references />
__NOTOC__
__NOTOC__
</noinclude>
===Categories===
===Categories===
{{BacktoTop}}
{{BacktoTop}}
[[Category:Txn_unit:dsrA]]
[[Category:Txn_unit:dsrA]]

Latest revision as of 08:19, 27 May 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Overview Transcription Translation Turnover Experimental Links References Suggestions

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

dsrA

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Figure courtesy of RegulonDB


Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


dsrAp is regulated by temperature and operates efficiently at 25 C but poorly at 42 C. [1]

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:2023317..2023357 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.


Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference

dsrAp construct Δ37

G to A at -8 relative to the transcription start site

temperature regulation abolished

PMID:14594834[1]

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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1954 (EcoliWiki Page)

NCBI GEO profiles for dsrA

microarray

GenExpDB:b1954 (EcoliWiki Page)

Summary of data for dsrA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (2023265..2023571) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ19; Well:B4[2]

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<protect>

Notes

Accessions Related to dsrA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7047

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4281

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1954

EcoGene

EcoGene:EG31118

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003633

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006490

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 Repoila, F & Gottesman, S (2003) Temperature sensing by the dsrA promoter. J. Bacteriol. 185 6609-14 PubMed
  2. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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