Number of molecules per cell: Difference between revisions

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!|Molecule!!Organism or Strain!!Molecules per cell!!Growth conditions!!Growth rate!!Assay used!!Notes!!Reference(s)
!|Molecule!!Organism or Strain!!Value!!Units!!Experimental Conditions!!Assay used!!Notes!!Reference(s)
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<i>E. coli</i>
<i>E. coli</i>
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~16
8 to 12 tetramers (32 to 48 monomers)
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Grown in tryptone-yeast extract medium, cells harvested "late in the log phase."
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*Medium: 
*Temperature (°C):  Yield obtained after the first steps of purification assayed by binding of radioactive IPTG.
*Doubling time (min): Growth Rate(min):  PMID:16591435
*Growth phase: other:
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LacI (Lac Repressor)
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<i>E. coli</i>
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8 to 12 tetramers (32 to 48 monomers)
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Grown in tryptone-yeast extract medium, cells harvested "late in the log phase."
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*Medium:
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Yield obtained after the first steps of purification assayed by binding of radioactive IPTG.
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PMID:16591435


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Latest revision as of 19:58, 21 July 2015

See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

LacI (Lac Repressor)

E. coli

8 to 12 tetramers (32 to 48 monomers)

Grown in tryptone-yeast extract medium, cells harvested "late in the log phase."

  • Medium:
  • Temperature (°C): Yield obtained after the first steps of purification assayed by binding of radioactive IPTG.
  • Doubling time (min): Growth Rate(min): PMID:16591435[1]
  • Growth phase: other:

LacI (Lac Repressor)

E. coli

8 to 12 tetramers (32 to 48 monomers)

Grown in tryptone-yeast extract medium, cells harvested "late in the log phase."

  • Medium:

Yield obtained after the first steps of purification assayed by binding of radioactive IPTG.

PMID:16591435[1]

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  1. ↑ 1.0 1.1 Gilbert, W & Müller-Hill, B (1966) Isolation of the lac repressor. Proc. Natl. Acad. Sci. U.S.A. 56 1891-8 PubMed
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