pGEX-4T-2: Difference between revisions

Latest revision as of 20:05, 18 August 2011

At a Glance

Type	Cloning
Origin of Replication
Copy #
Markers	Ampicillin
Link to Sequence	NCBI
Features	tac Promoter lac Operator RBS MCS lacIq
Accession Number	U13854
edit table

Notes

pGEX-4T-2 is derived from pGEX-2T and contain a thrombin recognition site. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

GE Healthcare

References

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See Help:References for how to manage references in EcoliWiki.

Sequence

       1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
      61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     901 atcctccaaa atcggatctg gttccgcgtg gatccccagg aattcccggg tcgactcgag
     961 cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga cggtgaaaac
    1021 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    1081 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
    1141 cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct cgtgatacgc
    1201 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
    1261 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
    1321 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
    1381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
    1441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    1501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    1561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    1621 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    1681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    1741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    1801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    1861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    1921 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    1981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    2041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    2101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    2161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    2221 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    2281 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    2341 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    2401 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    2461 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    2521 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
    2581 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    2641 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    2701 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    2761 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    2821 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    2881 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    2941 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    3001 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    3061 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    3121 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    3181 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataaattcc
    3241 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
    3301 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
    3361 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
    3421 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
    3481 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
    3541 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
    3601 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
    3661 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
    3721 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
    3781 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
    3841 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
    3901 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
    3961 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
    4021 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
    4081 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
    4141 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
    4201 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
    4261 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
    4321 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    4381 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    4441 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    4501 acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac
    4561 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    4621 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    4681 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
    4741 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
    4801 tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc
    4861 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
    4921 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttggaatt

@@ Line 1: / Line 1: @@
 ==At a Glance==
-[[Image:#PGEX.jpg|right|thumb|600px|pGEX-4T-1; Image provided by  [http://www6.gelifesciences.com/aptrix/upp00919.nsf/Content/WD%3ApGEX+Vectors%2C+G%28363951779-R350%29?OpenDocument GE Healthcare]]]
+[[File:PGEX-4T-2.jpg|thumb|450px|pGEX-4T-2, Image made with [[PlasMapper]]]]
 <protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.V480cf3e4d709b-->
 <!--
 ******************************************************************************************
 *
 *   ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 *
 ****************************************************************************************** -->
-{|{{Prettytable}} width='30%' align=left
+{| {{Prettytable}} width='30%' align=left  id="V480cf3e4d709b"  class=" tableEdit PlasmidTable"
 |-
-!{{table heading style}} |Type
+!align=left {{table heading style}} |Type
-|
+||
 Cloning
 |-
-!{{table heading style}} |Origin of Replication
+!align=left {{table heading style}} |Origin of Replication
-|
+||
 |-
-!{{table heading style}} |Copy #
+!align=left {{table heading style}} |Copy #
-|
+||
 |-
-!{{table heading style}} |Markers
+!align=left {{table heading style}} |Markers
-|
+||
 Ampicillin
 |-
-!{{table heading style}} |Link to Sequence
+!align=left {{table heading style}} |Link to Sequence
-|
+||
-[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=U13854 PubMed]
+[http://www.ncbi.nlm.nih.gov/nuccore/595725?report=fasta NCBI]
 |-
-!{{table heading style}} |Features
+!align=left {{table heading style}} |Features
-|
+||
-tac Promoter<br>lac Operator<br>RBS<br>MCS<br>lacIq
+tac Promoter<br />
+lac Operator<br />
+RBS<br />
+MCS<br />
+lacIq
 |-
-!{{table heading style}} |Accession Number
+!align=left {{table heading style}} |Accession Number
-|
+||
 [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=U13854 U13854]
-|-
-|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.V480cf3e4d709b&page=0&pagename={{FULLPAGENAMEE}} edit table]
+|- class="tableEdit_footer"
+|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.V480cf3e4d709b&page=134062&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> ||
 |}
 <!--box uid=63ce1649aab8b3180235b0af7618f667.0.V480cf3e4d709b--></protect>
 <BR>
 <Br clear='all'>
 ==Notes==
 pGEX-4T-2 is derived from pGEX-2T and contain a thrombin recognition site.  The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors.
@@ Line 49: / Line 56: @@
 {{PlasmidHistory}}
-==Sources {{HelpIcon|Help:Plasmid}}==
+==Sources==
-{{PlasmidSources}}
+[http://www6.gelifesciences.com/APTRIX/upp01077.nsf/Content/Products?OpenDocument&parentid=366157&moduleid=38859&zone=Proteomics GE Healthcare]
 ==References==
+{{BacktoTop}}
 {{RefHelp}}
-<references/>
+<references />
+==Sequence==
+acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
+gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
+tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
+tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
+cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
+aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
+gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
+ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
+tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
+ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
+ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
+atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
+tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
+aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
+ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
+atcctccaaa atcggatctg gttccgcgtg gatccccagg aattcccggg tcgactcgag
+cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga cggtgaaaac
+ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
+agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
+cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct cgtgatacgc
+ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
+cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
+ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
+agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
+tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
+gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
+gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
+gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
+gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
+agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
+ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
+cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
+gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
+cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
+gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
+ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
+acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
+ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
+aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
+aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
+ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
+ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
+actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
+caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
+gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
+ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
+cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
+cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
+acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
+ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
+gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
+tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
+accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
+cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataaattcc
+gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
+caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
+gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
+cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
+caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
+gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
+gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
+ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
+gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
+cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
+gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
+cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
+gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
+gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
+cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
+gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
+ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
+ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
+caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
+cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
+accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
+acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac
+aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
+ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
+gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
+gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
+tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc
+cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
+gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttggaatt
-==Sequence {{HelpIcon|Help:Plasmid}}==
-{{PlasmidSeq}}
-[[Category:Plasmids]]
+[[Category:Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Cloning Plasmids]][[Category:tac Promoter]][[Category:lac Operator]][[Category:lacIq]][[Category:GE Healthcare]]
 __NOTOC__