pGEX-4T-2
At a Glance
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Type |
Cloning |
---|---|
Origin of Replication | |
Copy # | |
Markers |
Ampicillin |
Link to Sequence | |
Features |
tac Promoter |
Accession Number | |
edit table |
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Notes
pGEX-4T-2 is derived from pGEX-2T and contain a thrombin recognition site. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors.
History
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Sources
References
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Sequence
1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccccagg aattcccggg tcgactcgag 961 cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga cggtgaaaac 1021 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 1081 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc 1141 cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct cgtgatacgc 1201 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt 1261 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 1321 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 1381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 1441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 1501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 1561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 1621 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 1681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 1741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 1801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 1861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 1921 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 1981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 2041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 2101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 2161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 2221 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 2281 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 2341 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 2401 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 2461 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 2521 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 2581 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 2641 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 2701 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 2761 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 2821 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 2881 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 2941 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 3001 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 3061 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 3121 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 3181 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataaattcc 3241 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt 3301 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt 3361 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg 3421 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa 3481 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac 3541 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg 3601 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt 3661 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt 3721 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca 3781 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg 3841 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg 3901 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg 3961 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat 4021 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg 4081 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac 4141 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc 4201 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 4261 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 4321 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 4381 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 4441 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 4501 acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac 4561 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 4621 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 4681 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa 4741 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga 4801 tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc 4861 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa 4921 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttggaatt