pGEX-4T-2

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At a Glance

pGEX-4T-2, Image made with PlasMapper

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Type

Cloning

Origin of Replication
Copy #
Markers

Ampicillin

Link to Sequence

NCBI

Features

tac Promoter
lac Operator
RBS
MCS
lacIq

Accession Number

U13854

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Notes

pGEX-4T-2 is derived from pGEX-2T and contain a thrombin recognition site. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors.

History

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Sources

GE Healthcare

References

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Sequence

       1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
      61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     901 atcctccaaa atcggatctg gttccgcgtg gatccccagg aattcccggg tcgactcgag
     961 cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga cggtgaaaac
    1021 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    1081 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
    1141 cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct cgtgatacgc
    1201 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
    1261 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
    1321 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
    1381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
    1441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    1501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    1561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    1621 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    1681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    1741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    1801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    1861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    1921 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    1981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    2041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    2101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    2161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    2221 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    2281 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    2341 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    2401 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    2461 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    2521 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
    2581 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    2641 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    2701 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    2761 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    2821 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    2881 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    2941 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    3001 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    3061 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    3121 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    3181 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataaattcc
    3241 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
    3301 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
    3361 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
    3421 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
    3481 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
    3541 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
    3601 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
    3661 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
    3721 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
    3781 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
    3841 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
    3901 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
    3961 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
    4021 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
    4081 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
    4141 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
    4201 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
    4261 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
    4321 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    4381 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    4441 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    4501 acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac
    4561 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    4621 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    4681 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
    4741 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
    4801 tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc
    4861 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
    4921 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttggaatt