pIndigoBAC-5: Difference between revisions

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==At a Glance {{HelpIcon|Help:Plasmid}}==
==At a Glance==
[[File:PIndigoBAC-5.jpg|thumb|450px|pIndigoBAC-5, Image made with [[PlasMapper]]]]
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="X471faa3d4c586"  class=" tableEdit PlasmidTable" 
 
|-
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!{{table heading style}} |Type
!align=left {{table heading style}} |Type
||
Cloning
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
||
 
|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
||
cannot be induced to high-copy number
|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
||
 
|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
||
[http://www.ncbi.nlm.nih.gov/nuccore/157065015? PubMed]
|-
|-
!{{table heading style}} |Comments
!align=left {{table heading style}} |Features
||
BamH I or Hind III Cloning Ready pIndigoBAC-5 Vectors;
pIndigoBAC-5 Vectors are supplied linearized at either unique BamH I or Hind III site, completely dephosphorylated, and highly purified. Vectors are ready for cloning BamH I- or Hind III-cut genomic DNA. 
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!align=left {{table heading style}} |Accession Number
||
[http://www.ncbi.nlm.nih.gov/nuccore/157065015? GenBank: EU140754.1]
 
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==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}


==Sources {{HelpIcon|Help:Plasmid}}==
==Notes==
{{PlasmidSources}}
 
==History==
pIndigoBAC-5 is derived from pBeloBAC11 and pIndigoBAC.<ref>Birren, B. et al. (1999) Bacterial Artificial Chromosomes in Genome Analysis: A Laboratory Manual v. 3, 241.</ref>
 
==Sources==
[http://www.epibio.com/item.asp?ID=567 Epicentre]


==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
 
==Sequence {{HelpIcon|Help:Plasmid}}==
{{PlasmidSeq}}


==Sequence==
<pre>
        1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
      61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
      121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
      181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatcctc
      361 tagagtcgac ctgcaggcat gcaagcttga gtattctata gtctcaccta aatagcttgg
      421 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      481 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      541 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      601 attaatgaat cggccaacgc gaaccccttg cggccgcccg ggccgtcgac caattctcat
      661 gtttgacagc ttatcatcga atttctgcca ttcatccgct tattatcact tattcaggcg
      721 tagcaaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc
      781 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      841 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      901 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      961 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
    1021 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
    1081 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
    1141 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
    1201 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
    1261 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
    1321 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
    1381 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
    1441 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
    1501 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc
    1561 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
    1621 ggtatttatt cgcgataagc tcatggagcg gcgtaaccgt cgcacaggaa ggacagagaa
    1681 agcgcggatc tgggaagtga cggacagaac ggtcaggacc tggattgggg aggcggttgc
    1741 cgccgctgct gctgacggtg tgacgttctc tgttccggtc acaccacata cgttccgcca
    1801 ttcctatgcg atgcacatgc tgtatgccgg tataccgctg aaagttctgc aaagcctgat
    1861 gggacataag tccatcagtt caacggaagt ctacacgaag gtttttgcgc tggatgtggc
    1921 tgcccggcac cgggtgcagt ttgcgatgcc ggagtctgat gcggttgcga tgctgaaaca
    1981 attatcctga gaataaatgc cttggccttt atatggaaat gtggaactga gtggatatgc
    2041 tgtttttgtc tgttaaacag agaagctggc tgttatccac tgagaagcga acgaaacagt
    2101 cgggaaaatc tcccattatc gtagagatcc gcattattaa tctcaggagc ctgtgtagcg
    2161 tttataggaa gtagtgttct gtcatgatgc ctgcaagcgg taacgaaaac gatttgaata
    2221 tgccttcagg aacaatagaa atcttcgtgc ggtgttacgt tgaagtggag cggattatgt
    2281 cagcaatgga cagaacaacc taatgaacac agaaccatga tgtggtctgt ccttttacag
    2341 ccagtagtgc tcgccgcagt cgagcgacag ggcgaagccc tcgagtgagc gaggaagcac
    2401 cagggaacag cacttatata ttctgcttac acacgatgcc tgaaaaaact tcccttgggg
    2461 ttatccactt atccacgggg atatttttat aattattttt tttatagttt ttagatcttc
    2521 ttttttagag cgccttgtag gcctttatcc atgctggttc tagagaaggt gttgtgacaa
    2581 attgcccttt cagtgtgaca aatcaccctc aaatgacagt cctgtctgtg acaaattgcc
    2641 cttaaccctg tgacaaattg ccctcagaag aagctgtttt ttcacaaagt tatccctgct
    2701 tattgactct tttttattta gtgtgacaat ctaaaaactt gtcacacttc acatggatct
    2761 gtcatggcgg aaacagcggt tatcaatcac aagaaacgta aaaatagccc gcgaatcgtc
    2821 cagtcaaacg acctcactga ggcggcatat agtctctccc gggatcaaaa acgtatgctg
    2881 tatctgttcg ttgaccagat cagaaaatct gatggcaccc tacaggaaca tgacggtatc
    2941 tgcgagatcc atgttgctaa atatgctgaa atattcggat tgacctctgc ggaagccagt
    3001 aaggatatac ggcaggcatt gaagagtttc gcggggaagg aagtggtttt ttatcgccct
    3061 gaagaggatg ccggcgatga aaaaggctat gaatcttttc cttggtttat caaacgtgcg
    3121 cacagtccat ccagagggct ttacagtgta catatcaacc catatctcat tcccttcttt
    3181 atcgggttac agaaccggtt tacgcagttt cggcttagtg aaacaaaaga aatcaccaat
    3241 ccgtatgcca tgcgtttata cgaatccctg tgtcagtatc gtaagccgga tggctcaggc
    3301 atcgtctctc tgaaaatcga ctggatcata gagcgttacc agctgcctca aagttaccag
    3361 cgtatgcctg acttccgccg ccgcttcctg caggtctgtg ttaatgagat caacagcaga
    3421 actccaatgc gcctctcata cattgagaaa aagaaaggcc gccagacgac tcatatcgta
    3481 ttttccttcc gcgatatcac ttccatgacg acaggatagt ctgagggtta tctgtcacag
    3541 atttgagggt ggttcgtcac atttgttctg acctactgag ggtaatttgt cacagttttg
    3601 ctgtttcctt cagcctgcat ggattttctc atactttttg aactgtaatt tttaaggaag
    3661 ccaaatttga gggcagtttg tcacagttga tttccttctc tttcccttcg tcatgtgacc
    3721 tgatatcggg ggttagttcg tcatcattga tgagggttga ttatcacagt ttattactct
    3781 gaattggcta tccgcgtgtg tacctctacc tggagttttt cccacggtgg atatttcttc
    3841 ttgcgctgag cgtaagagct atctgacaga acagttcttc tttgcttcct cgccagttcg
    3901 ctcgctatgc tcggttacac ggctgcggcg agcgctagtg ataataagtg actgaggtat
    3961 gtgctcttct tatctccttt tgtagtgttg ctcttatttt aaacaacttt gcggtttttt
    4021 gatgactttg cgattttgtt gttgctttgc agtaaattgc aagatttaat aaaaaaacgc
    4081 aaagcaatga ttaaaggatg ttcagaatga aactcatgga aacacttaac cagtgcataa
    4141 acgctggtca tgaaatgacg aaggctatcg ccattgcaca gtttaatgat gacagcccgg
    4201 aagcgaggaa aataacccgg cgctggagaa taggtgaagc agcggattta gttggggttt
    4261 cttctcaggc tatcagagat gccgagaaag cagggcgact accgcacccg gatatggaaa
    4321 ttcgaggacg ggttgagcaa cgtgttggtt atacaattga acaaattaat catatgcgtg
    4381 atgtgtttgg tacgcgattg cgacgtgctg aagacgtatt tccaccggtg atcggggttg
    4441 ctgcccataa aggtggcgtt tacaaaacct cagtttctgt tcatcttgct caggatctgg
    4501 ctctgaaggg gctacgtgtt ttgctcgtgg aaggtaacga cccccaggga acagcctcaa
    4561 tgtatcacgg atgggtacca gatcttcata ttcatgcaga agacactctc ctgcctttct
    4621 atcttgggga aaaggacgat gtcacttatg caataaagcc cacttgctgg ccggggcttg
    4681 acattattcc ttcctgtctg gctctgcacc gtattgaaac tgagttaatg ggcaaatttg
    4741 atgaaggtaa actgcccacc gatccacacc tgatgctccg actggccatt gaaactgttg
    4801 ctcatgacta tgatgtcata gttattgaca gcgcgcctaa cctgggtatc ggcacgatta
    4861 atgtcgtatg tgctgctgat gtgctgattg ttcccacgcc tgctgagttg tttgactaca
    4921 cctccgcact gcagtttttc gatatgcttc gtgatctgct caagaacgtt gatcttaaag
    4981 ggttcgagcc tgatgtacgt attttgctta ccaaatacag caatagtaat ggctctcagt
    5041 ccccgtggat ggaggagcaa attcgggatg cctggggaag catggttcta aaaaatgttg
    5101 tacgtgaaac ggatgaagtt ggtaaaggtc agatccggat gagaactgtt tttgaacagg
    5161 ccattgatca acgctcttca actggtgcct ggagaaatgc tctttctatt tgggaacctg
    5221 tctgcaatga aattttcgat cgtctgatta aaccacgctg ggagattaga taatgaagcg
    5281 tgcgcctgtt attccaaaac atacgctcaa tactcaaccg gttgaagata cttcgttatc
    5341 gacaccagct gccccgatgg tggattcgtt aattgcgcgc gtaggagtaa tggctcgcgg
    5401 taatgccatt actttgcctg tatgtggtcg ggatgtgaag tttactcttg aagtgctccg
    5461 gggtgatagt gttgagaaga cctctcgggt atggtcaggt aatgaacgtg accaggagct
    5521 gcttactgag gacgcactgg atgatctcat cccttctttt ctactgactg gtcaacagac
    5581 accggcgttc ggtcgaagag tatctggtgt catagaaatt gccgatggga gtcgccgtcg
    5641 taaagctgct gcacttaccg aaagtgatta tcgtgttctg gttggcgagc tggatgatga
    5701 gcagatggct gcattatcca gattgggtaa cgattatcgc ccaacaagtg cttatgaacg
    5761 tggtcagcgt tatgcaagcc gattgcagaa tgaatttgct ggaaatattt ctgcgctggc
    5821 tgatgcggaa aatatttcac gtaagattat tacccgctgt atcaacaccg ccaaattgcc
    5881 taaatcagtt gttgctcttt tttctcaccc cggtgaacta tctgcccggt caggtgatgc
    5941 acttcaaaaa gcctttacag ataaagagga attacttaag cagcaggcat ctaaccttca
    6001 tgagcagaaa aaagctgggg tgatatttga agctgaagaa gttatcactc ttttaacttc
    6061 tgtgcttaaa acgtcatctg catcaagaac tagtttaagc tcacgacatc agtttgctcc
    6121 tggagcgaca gtattgtata agggcgataa aatggtgctt aacctggaca ggtctcgtgt
    6181 tccaactgag tgtatagaga aaattgaggc cattcttaag gaacttgaaa agccagcacc
    6241 ctgatgcgac cacgttttag tctacgttta tctgtcttta cttaatgtcc tttgttacag
    6301 gccagaaagc ataactggcc tgaatattct ctctgggccc actgttccac ttgtatcgtc
    6361 ggtctgataa tcagactggg accacggtcc cactcgtatc gtcggtctga ttattagtct
    6421 gggaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact
    6481 cgtatcgtcg gtctgataat cagactggga ccacggtccc actcgtatcg tcggtctgat
    6541 tattagtctg ggaccatggt cccactcgta tcgtcggtct gattattagt ctgggaccac
    6601 ggtcccactc gtatcgtcgg tctgattatt agtctggaac cacggtccca ctcgtatcgt
    6661 cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg attattagtc
    6721 tgggaccacg atcccactcg tgttgtcggt ctgattatcg gtctgggacc acggtcccac
    6781 ttgtattgtc gatcagacta tcagcgtgag actacgattc catcaatgcc tgtcaagggc
    6841 aagtattgac atgtcgtcgt aacctgtaga acggagtaac ctcggtgtgc ggttgtatgc
    6901 ctgctgtgga ttgctgctgt gtcctgctta tccacaacat tttgcgcacg gttatgtgga
    6961 caaaatacct ggttacccag gccgtgccgg cacgttaacc gggctgcatc cgatgcaagt
    7021 gtgtcgctgt cgacgagctc gcgagctcgg acatgaggtt gccccgtatt cagtgtcgct
    7081 gatttgtatt gtctgaagtt gtttttacgt taagttgatg cagatcaatt aatacgatac
    7141 ctgcgtcata attgattatt tgacgtggtt tgatggcctc cacgcacgtt gtgatatgta
    7201 gatgataatc attatcactt tacgggtcct ttccggtgat ccgacaggtt acggggcggc
    7261 gacctcgcgg gttttcgcta tttatgaaaa ttttccggtt taaggcgttt ccgttcttct
    7321 tcgtcataac ttaatgtttt tatttaaaat accctctgaa aagaaaggaa acgacaggtg
    7381 ctgaaagcga gctttttggc ctctgtcgtt tcctttctct gtttttgtcc gtggaatgaa
    7441 caatggaagt ccgagctcat cgctaataac ttcgtatagc atacattata cgaagttata
    7501 ttcgat


</pre>




[[Category:Plasmids]]
[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Epicentre Plasmids]]
__NOTOC__
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Latest revision as of 22:03, 29 August 2011

At a Glance

pIndigoBAC-5, Image made with PlasMapper
Type

Cloning

Origin of Replication
Copy #

cannot be induced to high-copy number

Markers
Link to Sequence

PubMed

Features

BamH I or Hind III Cloning Ready pIndigoBAC-5 Vectors; pIndigoBAC-5 Vectors are supplied linearized at either unique BamH I or Hind III site, completely dephosphorylated, and highly purified. Vectors are ready for cloning BamH I- or Hind III-cut genomic DNA.

Accession Number

GenBank: EU140754.1

edit table



Notes

History

pIndigoBAC-5 is derived from pBeloBAC11 and pIndigoBAC.[1]

Sources

Epicentre

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ Birren, B. et al. (1999) Bacterial Artificial Chromosomes in Genome Analysis: A Laboratory Manual v. 3, 241.

Sequence

        1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
       61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
      121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
      181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatcctc
      361 tagagtcgac ctgcaggcat gcaagcttga gtattctata gtctcaccta aatagcttgg
      421 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      481 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      541 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      601 attaatgaat cggccaacgc gaaccccttg cggccgcccg ggccgtcgac caattctcat
      661 gtttgacagc ttatcatcga atttctgcca ttcatccgct tattatcact tattcaggcg
      721 tagcaaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc
      781 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      841 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      901 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      961 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     1021 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     1081 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     1141 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     1201 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     1261 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     1321 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     1381 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     1441 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1501 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc
     1561 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
     1621 ggtatttatt cgcgataagc tcatggagcg gcgtaaccgt cgcacaggaa ggacagagaa
     1681 agcgcggatc tgggaagtga cggacagaac ggtcaggacc tggattgggg aggcggttgc
     1741 cgccgctgct gctgacggtg tgacgttctc tgttccggtc acaccacata cgttccgcca
     1801 ttcctatgcg atgcacatgc tgtatgccgg tataccgctg aaagttctgc aaagcctgat
     1861 gggacataag tccatcagtt caacggaagt ctacacgaag gtttttgcgc tggatgtggc
     1921 tgcccggcac cgggtgcagt ttgcgatgcc ggagtctgat gcggttgcga tgctgaaaca
     1981 attatcctga gaataaatgc cttggccttt atatggaaat gtggaactga gtggatatgc
     2041 tgtttttgtc tgttaaacag agaagctggc tgttatccac tgagaagcga acgaaacagt
     2101 cgggaaaatc tcccattatc gtagagatcc gcattattaa tctcaggagc ctgtgtagcg
     2161 tttataggaa gtagtgttct gtcatgatgc ctgcaagcgg taacgaaaac gatttgaata
     2221 tgccttcagg aacaatagaa atcttcgtgc ggtgttacgt tgaagtggag cggattatgt
     2281 cagcaatgga cagaacaacc taatgaacac agaaccatga tgtggtctgt ccttttacag
     2341 ccagtagtgc tcgccgcagt cgagcgacag ggcgaagccc tcgagtgagc gaggaagcac
     2401 cagggaacag cacttatata ttctgcttac acacgatgcc tgaaaaaact tcccttgggg
     2461 ttatccactt atccacgggg atatttttat aattattttt tttatagttt ttagatcttc
     2521 ttttttagag cgccttgtag gcctttatcc atgctggttc tagagaaggt gttgtgacaa
     2581 attgcccttt cagtgtgaca aatcaccctc aaatgacagt cctgtctgtg acaaattgcc
     2641 cttaaccctg tgacaaattg ccctcagaag aagctgtttt ttcacaaagt tatccctgct
     2701 tattgactct tttttattta gtgtgacaat ctaaaaactt gtcacacttc acatggatct
     2761 gtcatggcgg aaacagcggt tatcaatcac aagaaacgta aaaatagccc gcgaatcgtc
     2821 cagtcaaacg acctcactga ggcggcatat agtctctccc gggatcaaaa acgtatgctg
     2881 tatctgttcg ttgaccagat cagaaaatct gatggcaccc tacaggaaca tgacggtatc
     2941 tgcgagatcc atgttgctaa atatgctgaa atattcggat tgacctctgc ggaagccagt
     3001 aaggatatac ggcaggcatt gaagagtttc gcggggaagg aagtggtttt ttatcgccct
     3061 gaagaggatg ccggcgatga aaaaggctat gaatcttttc cttggtttat caaacgtgcg
     3121 cacagtccat ccagagggct ttacagtgta catatcaacc catatctcat tcccttcttt
     3181 atcgggttac agaaccggtt tacgcagttt cggcttagtg aaacaaaaga aatcaccaat
     3241 ccgtatgcca tgcgtttata cgaatccctg tgtcagtatc gtaagccgga tggctcaggc
     3301 atcgtctctc tgaaaatcga ctggatcata gagcgttacc agctgcctca aagttaccag
     3361 cgtatgcctg acttccgccg ccgcttcctg caggtctgtg ttaatgagat caacagcaga
     3421 actccaatgc gcctctcata cattgagaaa aagaaaggcc gccagacgac tcatatcgta
     3481 ttttccttcc gcgatatcac ttccatgacg acaggatagt ctgagggtta tctgtcacag
     3541 atttgagggt ggttcgtcac atttgttctg acctactgag ggtaatttgt cacagttttg
     3601 ctgtttcctt cagcctgcat ggattttctc atactttttg aactgtaatt tttaaggaag
     3661 ccaaatttga gggcagtttg tcacagttga tttccttctc tttcccttcg tcatgtgacc
     3721 tgatatcggg ggttagttcg tcatcattga tgagggttga ttatcacagt ttattactct
     3781 gaattggcta tccgcgtgtg tacctctacc tggagttttt cccacggtgg atatttcttc
     3841 ttgcgctgag cgtaagagct atctgacaga acagttcttc tttgcttcct cgccagttcg
     3901 ctcgctatgc tcggttacac ggctgcggcg agcgctagtg ataataagtg actgaggtat
     3961 gtgctcttct tatctccttt tgtagtgttg ctcttatttt aaacaacttt gcggtttttt
     4021 gatgactttg cgattttgtt gttgctttgc agtaaattgc aagatttaat aaaaaaacgc
     4081 aaagcaatga ttaaaggatg ttcagaatga aactcatgga aacacttaac cagtgcataa
     4141 acgctggtca tgaaatgacg aaggctatcg ccattgcaca gtttaatgat gacagcccgg
     4201 aagcgaggaa aataacccgg cgctggagaa taggtgaagc agcggattta gttggggttt
     4261 cttctcaggc tatcagagat gccgagaaag cagggcgact accgcacccg gatatggaaa
     4321 ttcgaggacg ggttgagcaa cgtgttggtt atacaattga acaaattaat catatgcgtg
     4381 atgtgtttgg tacgcgattg cgacgtgctg aagacgtatt tccaccggtg atcggggttg
     4441 ctgcccataa aggtggcgtt tacaaaacct cagtttctgt tcatcttgct caggatctgg
     4501 ctctgaaggg gctacgtgtt ttgctcgtgg aaggtaacga cccccaggga acagcctcaa
     4561 tgtatcacgg atgggtacca gatcttcata ttcatgcaga agacactctc ctgcctttct
     4621 atcttgggga aaaggacgat gtcacttatg caataaagcc cacttgctgg ccggggcttg
     4681 acattattcc ttcctgtctg gctctgcacc gtattgaaac tgagttaatg ggcaaatttg
     4741 atgaaggtaa actgcccacc gatccacacc tgatgctccg actggccatt gaaactgttg
     4801 ctcatgacta tgatgtcata gttattgaca gcgcgcctaa cctgggtatc ggcacgatta
     4861 atgtcgtatg tgctgctgat gtgctgattg ttcccacgcc tgctgagttg tttgactaca
     4921 cctccgcact gcagtttttc gatatgcttc gtgatctgct caagaacgtt gatcttaaag
     4981 ggttcgagcc tgatgtacgt attttgctta ccaaatacag caatagtaat ggctctcagt
     5041 ccccgtggat ggaggagcaa attcgggatg cctggggaag catggttcta aaaaatgttg
     5101 tacgtgaaac ggatgaagtt ggtaaaggtc agatccggat gagaactgtt tttgaacagg
     5161 ccattgatca acgctcttca actggtgcct ggagaaatgc tctttctatt tgggaacctg
     5221 tctgcaatga aattttcgat cgtctgatta aaccacgctg ggagattaga taatgaagcg
     5281 tgcgcctgtt attccaaaac atacgctcaa tactcaaccg gttgaagata cttcgttatc
     5341 gacaccagct gccccgatgg tggattcgtt aattgcgcgc gtaggagtaa tggctcgcgg
     5401 taatgccatt actttgcctg tatgtggtcg ggatgtgaag tttactcttg aagtgctccg
     5461 gggtgatagt gttgagaaga cctctcgggt atggtcaggt aatgaacgtg accaggagct
     5521 gcttactgag gacgcactgg atgatctcat cccttctttt ctactgactg gtcaacagac
     5581 accggcgttc ggtcgaagag tatctggtgt catagaaatt gccgatggga gtcgccgtcg
     5641 taaagctgct gcacttaccg aaagtgatta tcgtgttctg gttggcgagc tggatgatga
     5701 gcagatggct gcattatcca gattgggtaa cgattatcgc ccaacaagtg cttatgaacg
     5761 tggtcagcgt tatgcaagcc gattgcagaa tgaatttgct ggaaatattt ctgcgctggc
     5821 tgatgcggaa aatatttcac gtaagattat tacccgctgt atcaacaccg ccaaattgcc
     5881 taaatcagtt gttgctcttt tttctcaccc cggtgaacta tctgcccggt caggtgatgc
     5941 acttcaaaaa gcctttacag ataaagagga attacttaag cagcaggcat ctaaccttca
     6001 tgagcagaaa aaagctgggg tgatatttga agctgaagaa gttatcactc ttttaacttc
     6061 tgtgcttaaa acgtcatctg catcaagaac tagtttaagc tcacgacatc agtttgctcc
     6121 tggagcgaca gtattgtata agggcgataa aatggtgctt aacctggaca ggtctcgtgt
     6181 tccaactgag tgtatagaga aaattgaggc cattcttaag gaacttgaaa agccagcacc
     6241 ctgatgcgac cacgttttag tctacgttta tctgtcttta cttaatgtcc tttgttacag
     6301 gccagaaagc ataactggcc tgaatattct ctctgggccc actgttccac ttgtatcgtc
     6361 ggtctgataa tcagactggg accacggtcc cactcgtatc gtcggtctga ttattagtct
     6421 gggaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact
     6481 cgtatcgtcg gtctgataat cagactggga ccacggtccc actcgtatcg tcggtctgat
     6541 tattagtctg ggaccatggt cccactcgta tcgtcggtct gattattagt ctgggaccac
     6601 ggtcccactc gtatcgtcgg tctgattatt agtctggaac cacggtccca ctcgtatcgt
     6661 cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg attattagtc
     6721 tgggaccacg atcccactcg tgttgtcggt ctgattatcg gtctgggacc acggtcccac
     6781 ttgtattgtc gatcagacta tcagcgtgag actacgattc catcaatgcc tgtcaagggc
     6841 aagtattgac atgtcgtcgt aacctgtaga acggagtaac ctcggtgtgc ggttgtatgc
     6901 ctgctgtgga ttgctgctgt gtcctgctta tccacaacat tttgcgcacg gttatgtgga
     6961 caaaatacct ggttacccag gccgtgccgg cacgttaacc gggctgcatc cgatgcaagt
     7021 gtgtcgctgt cgacgagctc gcgagctcgg acatgaggtt gccccgtatt cagtgtcgct
     7081 gatttgtatt gtctgaagtt gtttttacgt taagttgatg cagatcaatt aatacgatac
     7141 ctgcgtcata attgattatt tgacgtggtt tgatggcctc cacgcacgtt gtgatatgta
     7201 gatgataatc attatcactt tacgggtcct ttccggtgat ccgacaggtt acggggcggc
     7261 gacctcgcgg gttttcgcta tttatgaaaa ttttccggtt taaggcgttt ccgttcttct
     7321 tcgtcataac ttaatgtttt tatttaaaat accctctgaa aagaaaggaa acgacaggtg
     7381 ctgaaagcga gctttttggc ctctgtcgtt tcctttctct gtttttgtcc gtggaatgaa
     7441 caatggaagt ccgagctcat cgctaataac ttcgtatagc atacattata cgaagttata
     7501 ttcgat
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