pIndigoBAC-5
At a Glance
pIndigoBAC-5, Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication | |
| Copy # |
cannot be induced to high-copy number |
| Markers | |
| Link to Sequence | |
| Features |
BamH I or Hind III Cloning Ready pIndigoBAC-5 Vectors; pIndigoBAC-5 Vectors are supplied linearized at either unique BamH I or Hind III site, completely dephosphorylated, and highly purified. Vectors are ready for cloning BamH I- or Hind III-cut genomic DNA. |
| Accession Number | |
| edit table |
</protect>
Notes
History
pIndigoBAC-5 is derived from pBeloBAC11 and pIndigoBAC.[1]
Sources
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Birren, B. et al. (1999) Bacterial Artificial Chromosomes in Genome Analysis: A Laboratory Manual v. 3, 241.
Sequence
1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatcctc
361 tagagtcgac ctgcaggcat gcaagcttga gtattctata gtctcaccta aatagcttgg
421 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
481 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
541 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
601 attaatgaat cggccaacgc gaaccccttg cggccgcccg ggccgtcgac caattctcat
661 gtttgacagc ttatcatcga atttctgcca ttcatccgct tattatcact tattcaggcg
721 tagcaaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc
781 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
841 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
901 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
961 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
1021 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
1081 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
1141 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
1201 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
1261 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
1321 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
1381 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
1441 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
1501 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc
1561 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
1621 ggtatttatt cgcgataagc tcatggagcg gcgtaaccgt cgcacaggaa ggacagagaa
1681 agcgcggatc tgggaagtga cggacagaac ggtcaggacc tggattgggg aggcggttgc
1741 cgccgctgct gctgacggtg tgacgttctc tgttccggtc acaccacata cgttccgcca
1801 ttcctatgcg atgcacatgc tgtatgccgg tataccgctg aaagttctgc aaagcctgat
1861 gggacataag tccatcagtt caacggaagt ctacacgaag gtttttgcgc tggatgtggc
1921 tgcccggcac cgggtgcagt ttgcgatgcc ggagtctgat gcggttgcga tgctgaaaca
1981 attatcctga gaataaatgc cttggccttt atatggaaat gtggaactga gtggatatgc
2041 tgtttttgtc tgttaaacag agaagctggc tgttatccac tgagaagcga acgaaacagt
2101 cgggaaaatc tcccattatc gtagagatcc gcattattaa tctcaggagc ctgtgtagcg
2161 tttataggaa gtagtgttct gtcatgatgc ctgcaagcgg taacgaaaac gatttgaata
2221 tgccttcagg aacaatagaa atcttcgtgc ggtgttacgt tgaagtggag cggattatgt
2281 cagcaatgga cagaacaacc taatgaacac agaaccatga tgtggtctgt ccttttacag
2341 ccagtagtgc tcgccgcagt cgagcgacag ggcgaagccc tcgagtgagc gaggaagcac
2401 cagggaacag cacttatata ttctgcttac acacgatgcc tgaaaaaact tcccttgggg
2461 ttatccactt atccacgggg atatttttat aattattttt tttatagttt ttagatcttc
2521 ttttttagag cgccttgtag gcctttatcc atgctggttc tagagaaggt gttgtgacaa
2581 attgcccttt cagtgtgaca aatcaccctc aaatgacagt cctgtctgtg acaaattgcc
2641 cttaaccctg tgacaaattg ccctcagaag aagctgtttt ttcacaaagt tatccctgct
2701 tattgactct tttttattta gtgtgacaat ctaaaaactt gtcacacttc acatggatct
2761 gtcatggcgg aaacagcggt tatcaatcac aagaaacgta aaaatagccc gcgaatcgtc
2821 cagtcaaacg acctcactga ggcggcatat agtctctccc gggatcaaaa acgtatgctg
2881 tatctgttcg ttgaccagat cagaaaatct gatggcaccc tacaggaaca tgacggtatc
2941 tgcgagatcc atgttgctaa atatgctgaa atattcggat tgacctctgc ggaagccagt
3001 aaggatatac ggcaggcatt gaagagtttc gcggggaagg aagtggtttt ttatcgccct
3061 gaagaggatg ccggcgatga aaaaggctat gaatcttttc cttggtttat caaacgtgcg
3121 cacagtccat ccagagggct ttacagtgta catatcaacc catatctcat tcccttcttt
3181 atcgggttac agaaccggtt tacgcagttt cggcttagtg aaacaaaaga aatcaccaat
3241 ccgtatgcca tgcgtttata cgaatccctg tgtcagtatc gtaagccgga tggctcaggc
3301 atcgtctctc tgaaaatcga ctggatcata gagcgttacc agctgcctca aagttaccag
3361 cgtatgcctg acttccgccg ccgcttcctg caggtctgtg ttaatgagat caacagcaga
3421 actccaatgc gcctctcata cattgagaaa aagaaaggcc gccagacgac tcatatcgta
3481 ttttccttcc gcgatatcac ttccatgacg acaggatagt ctgagggtta tctgtcacag
3541 atttgagggt ggttcgtcac atttgttctg acctactgag ggtaatttgt cacagttttg
3601 ctgtttcctt cagcctgcat ggattttctc atactttttg aactgtaatt tttaaggaag
3661 ccaaatttga gggcagtttg tcacagttga tttccttctc tttcccttcg tcatgtgacc
3721 tgatatcggg ggttagttcg tcatcattga tgagggttga ttatcacagt ttattactct
3781 gaattggcta tccgcgtgtg tacctctacc tggagttttt cccacggtgg atatttcttc
3841 ttgcgctgag cgtaagagct atctgacaga acagttcttc tttgcttcct cgccagttcg
3901 ctcgctatgc tcggttacac ggctgcggcg agcgctagtg ataataagtg actgaggtat
3961 gtgctcttct tatctccttt tgtagtgttg ctcttatttt aaacaacttt gcggtttttt
4021 gatgactttg cgattttgtt gttgctttgc agtaaattgc aagatttaat aaaaaaacgc
4081 aaagcaatga ttaaaggatg ttcagaatga aactcatgga aacacttaac cagtgcataa
4141 acgctggtca tgaaatgacg aaggctatcg ccattgcaca gtttaatgat gacagcccgg
4201 aagcgaggaa aataacccgg cgctggagaa taggtgaagc agcggattta gttggggttt
4261 cttctcaggc tatcagagat gccgagaaag cagggcgact accgcacccg gatatggaaa
4321 ttcgaggacg ggttgagcaa cgtgttggtt atacaattga acaaattaat catatgcgtg
4381 atgtgtttgg tacgcgattg cgacgtgctg aagacgtatt tccaccggtg atcggggttg
4441 ctgcccataa aggtggcgtt tacaaaacct cagtttctgt tcatcttgct caggatctgg
4501 ctctgaaggg gctacgtgtt ttgctcgtgg aaggtaacga cccccaggga acagcctcaa
4561 tgtatcacgg atgggtacca gatcttcata ttcatgcaga agacactctc ctgcctttct
4621 atcttgggga aaaggacgat gtcacttatg caataaagcc cacttgctgg ccggggcttg
4681 acattattcc ttcctgtctg gctctgcacc gtattgaaac tgagttaatg ggcaaatttg
4741 atgaaggtaa actgcccacc gatccacacc tgatgctccg actggccatt gaaactgttg
4801 ctcatgacta tgatgtcata gttattgaca gcgcgcctaa cctgggtatc ggcacgatta
4861 atgtcgtatg tgctgctgat gtgctgattg ttcccacgcc tgctgagttg tttgactaca
4921 cctccgcact gcagtttttc gatatgcttc gtgatctgct caagaacgtt gatcttaaag
4981 ggttcgagcc tgatgtacgt attttgctta ccaaatacag caatagtaat ggctctcagt
5041 ccccgtggat ggaggagcaa attcgggatg cctggggaag catggttcta aaaaatgttg
5101 tacgtgaaac ggatgaagtt ggtaaaggtc agatccggat gagaactgtt tttgaacagg
5161 ccattgatca acgctcttca actggtgcct ggagaaatgc tctttctatt tgggaacctg
5221 tctgcaatga aattttcgat cgtctgatta aaccacgctg ggagattaga taatgaagcg
5281 tgcgcctgtt attccaaaac atacgctcaa tactcaaccg gttgaagata cttcgttatc
5341 gacaccagct gccccgatgg tggattcgtt aattgcgcgc gtaggagtaa tggctcgcgg
5401 taatgccatt actttgcctg tatgtggtcg ggatgtgaag tttactcttg aagtgctccg
5461 gggtgatagt gttgagaaga cctctcgggt atggtcaggt aatgaacgtg accaggagct
5521 gcttactgag gacgcactgg atgatctcat cccttctttt ctactgactg gtcaacagac
5581 accggcgttc ggtcgaagag tatctggtgt catagaaatt gccgatggga gtcgccgtcg
5641 taaagctgct gcacttaccg aaagtgatta tcgtgttctg gttggcgagc tggatgatga
5701 gcagatggct gcattatcca gattgggtaa cgattatcgc ccaacaagtg cttatgaacg
5761 tggtcagcgt tatgcaagcc gattgcagaa tgaatttgct ggaaatattt ctgcgctggc
5821 tgatgcggaa aatatttcac gtaagattat tacccgctgt atcaacaccg ccaaattgcc
5881 taaatcagtt gttgctcttt tttctcaccc cggtgaacta tctgcccggt caggtgatgc
5941 acttcaaaaa gcctttacag ataaagagga attacttaag cagcaggcat ctaaccttca
6001 tgagcagaaa aaagctgggg tgatatttga agctgaagaa gttatcactc ttttaacttc
6061 tgtgcttaaa acgtcatctg catcaagaac tagtttaagc tcacgacatc agtttgctcc
6121 tggagcgaca gtattgtata agggcgataa aatggtgctt aacctggaca ggtctcgtgt
6181 tccaactgag tgtatagaga aaattgaggc cattcttaag gaacttgaaa agccagcacc
6241 ctgatgcgac cacgttttag tctacgttta tctgtcttta cttaatgtcc tttgttacag
6301 gccagaaagc ataactggcc tgaatattct ctctgggccc actgttccac ttgtatcgtc
6361 ggtctgataa tcagactggg accacggtcc cactcgtatc gtcggtctga ttattagtct
6421 gggaccacgg tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact
6481 cgtatcgtcg gtctgataat cagactggga ccacggtccc actcgtatcg tcggtctgat
6541 tattagtctg ggaccatggt cccactcgta tcgtcggtct gattattagt ctgggaccac
6601 ggtcccactc gtatcgtcgg tctgattatt agtctggaac cacggtccca ctcgtatcgt
6661 cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg attattagtc
6721 tgggaccacg atcccactcg tgttgtcggt ctgattatcg gtctgggacc acggtcccac
6781 ttgtattgtc gatcagacta tcagcgtgag actacgattc catcaatgcc tgtcaagggc
6841 aagtattgac atgtcgtcgt aacctgtaga acggagtaac ctcggtgtgc ggttgtatgc
6901 ctgctgtgga ttgctgctgt gtcctgctta tccacaacat tttgcgcacg gttatgtgga
6961 caaaatacct ggttacccag gccgtgccgg cacgttaacc gggctgcatc cgatgcaagt
7021 gtgtcgctgt cgacgagctc gcgagctcgg acatgaggtt gccccgtatt cagtgtcgct
7081 gatttgtatt gtctgaagtt gtttttacgt taagttgatg cagatcaatt aatacgatac
7141 ctgcgtcata attgattatt tgacgtggtt tgatggcctc cacgcacgtt gtgatatgta
7201 gatgataatc attatcactt tacgggtcct ttccggtgat ccgacaggtt acggggcggc
7261 gacctcgcgg gttttcgcta tttatgaaaa ttttccggtt taaggcgttt ccgttcttct
7321 tcgtcataac ttaatgtttt tatttaaaat accctctgaa aagaaaggaa acgacaggtg
7381 ctgaaagcga gctttttggc ctctgtcgtt tcctttctct gtttttgtcc gtggaatgaa
7441 caatggaagt ccgagctcat cgctaataac ttcgtatagc atacattata cgaagttata
7501 ttcgat
