pCC1BAC: Difference between revisions

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==At a Glance {{HelpIcon|Help:Plasmid}}==
==At a Glance==
[[File:PCC18BAC.jpg|thumb|450px|pCC1BAC, Image made with [[PlasMapper]]]]
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="S471f7ff09eda5"  class=" tableEdit PlasmidTable" 
 
|-
|-
!{{table heading style}} |Type
!align=left {{table heading style}} |Type
|
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Cloning
Cloning
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
|
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single-copy F-factor, multi-copy oriV  
single-copy F-factor, multi-copy oriV  
|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
|
||
single copy and can be induced to 10-20 copies per cell
single copy and can be induced to 10-20 copies per cell
|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
|
||


|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
|
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=157065011 PubMed]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=157065011 PubMed]
|-
|-
!{{table heading style}} |Comments
!align=left {{table heading style}} |Features
|
||
Copy Control Vector;  
Copy Control BamH I, EcoR I, or Hind III Cloning Ready pCC1BAC Vectors;  
For oriV to function as an origin of replication, oriV requires a gene product called "trfA".  Promega has genetically engineered E.coli strains to have the trfA gene under the control of a tightly-regulated inducible promoter.  Each Cloning Ready pCC1BAC Vector has been linearized at a unique BamH 1, EcoR 1 or Hind III site.
For oriV to function as an origin of replication, oriV requires a gene product called "trfA".  Promega has genetically engineered E.coli strains to have the trfA gene under the control of a tightly-regulated inducible promoter.
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!align=left {{table heading style}} |Accession Number
||
[http://www.ncbi.nlm.nih.gov/nuccore/157065011? GenBank: EU140750.1]
 
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==Notes==
==History {{HelpIcon|Help:Plasmid}}==
==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
{{PlasmidHistory}}


==Sources {{HelpIcon|Help:Plasmid}}==
==Sources==
{{PlasmidSources}}
[http://www.epibio.com/item.asp?id=380 Epicentre]


==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
 
==Sequence {{HelpIcon|Help:Plasmid}}==
{{PlasmidSeq}}


==Sequence==
<pre>   
        1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
      61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccacacagat
      121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
      181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatcctc
      361 tagagtcgac ctgcaggcat gcaagcttga gtattctata gtctcaccta aatagcttgg
      421 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      481 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      541 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      601 attaatgaat cggccaacgc gaaccccttg cggccgcccg ggccgtcgac caattctcat
      661 gtttgacagc ttatcatcga atttctgcca ttcatccgct tattatcact tattcaggcg
      721 tagcaaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc
      781 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      841 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      901 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      961 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
    1021 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
    1081 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
    1141 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
    1201 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
    1261 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
    1321 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
    1381 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
    1441 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
    1501 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc
    1561 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
    1621 ggtatttatt cgcgataagc tcatggagcg gcgtaaccgt cgcacaggaa ggacagagaa
    1681 agcgcggatc tgggaagtga cggacagaac ggtcaggacc tggattgggg aggcggttgc
    1741 cgccgctgct gctgacggtg tgacgttctc tgttccggtc acaccacata cgttccgcca
    1801 ttcctatgcg atgcacatgc tgtatgccgg tataccgctg aaagttctgc aaagcctgat
    1861 gggacataag tccatcagtt caacggaagt ctacacgaag gtttttgcgc tggatgtggc
    1921 tgcccggcac cgggtgcagt ttgcgatgcc ggagtctgat gcggttgcga tgctgaaaca
    1981 attatcctga gaataaatgc cttggccttt atatggaaat gtggaactga gtggatatgc
    2041 tgtttttgtc tgttaaacag agaagctggc tgttatccac tgagaagcga acgaaacagt
    2101 cgggaaaatc tcccattatc gtagagatcc gcattattaa tctcaggagc ctgtgtagcg
    2161 tttataggaa gtagtgttct gtcatgatgc ctgcaagcgg taacgaaaac gatttgaata
    2221 tgccttcagg aacaatagaa atcttcgtgc ggtgttacgt tgaagtggag cggattatgt
    2281 cagcaatgga cagaacaacc taatgaacac agaaccatga tgtggtctgt ccttttacag
    2341 ccagtagtgc tcgccgcagt cgagcgacag ggcgaagccc tcgagctggt tgccctcgcc
    2401 gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt
    2461 gcgagacacc gcggccggcc gccggcgttg tggatacctc gcggaaaact tggccctcac
    2521 tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac
    2581 agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg
    2641 cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag
    2701 tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt
    2761 gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct
    2821 gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta
    2881 acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg
    2941 cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc
    3001 ggtcgagtga gcgaggaagc accagggaac agcacttata tattctgctt acacacgatg
    3061 cctgaaaaaa cttcccttgg ggttatccac ttatccacgg ggatattttt ataattattt
    3121 tttttatagt ttttagatct tcttttttag agcgccttgt aggcctttat ccatgctggt
    3181 tctagagaag gtgttgtgac aaattgccct ttcagtgtga caaatcaccc tcaaatgaca
    3241 gtcctgtctg tgacaaattg cccttaaccc tgtgacaaat tgccctcaga agaagctgtt
    3301 ttttcacaaa gttatccctg cttattgact cttttttatt tagtgtgaca atctaaaaac
    3361 ttgtcacact tcacatggat ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg
    3421 taaaaatagc ccgcgaatcg tccagtcaaa cgacctcact gaggcggcat atagtctctc
    3481 ccgggatcaa aaacgtatgc tgtatctgtt cgttgaccag atcagaaaat ctgatggcac
    3541 cctacaggaa catgacggta tctgcgagat ccatgttgct aaatatgctg aaatattcgg
    3601 attgacctct gcggaagcca gtaaggatat acggcaggca ttgaagagtt tcgcggggaa
    3661 ggaagtggtt ttttatcgcc ctgaagagga tgccggcgat gaaaaaggct atgaatcttt
    3721 tccttggttt atcaaacgtg cgcacagtcc atccagaggg ctttacagtg tacatatcaa
    3781 cccatatctc attcccttct ttatcgggtt acagaaccgg tttacgcagt ttcggcttag
    3841 tgaaacaaaa gaaatcacca atccgtatgc catgcgttta tacgaatccc tgtgtcagta
    3901 tcgtaagccg gatggctcag gcatcgtctc tctgaaaatc gactggatca tagagcgtta
    3961 ccagctgcct caaagttacc agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg
    4021 tgttaatgag atcaacagca gaactccaat gcgcctctca tacattgaga aaaagaaagg
    4081 ccgccagacg actcatatcg tattttcctt ccgcgatatc acttccatga cgacaggata
    4141 gtctgagggt tatctgtcac agatttgagg gtggttcgtc acatttgttc tgacctactg
    4201 agggtaattt gtcacagttt tgctgtttcc ttcagcctgc atggattttc tcatactttt
    4261 tgaactgtaa tttttaagga agccaaattt gagggcagtt tgtcacagtt gatttccttc
    4321 tctttccctt cgtcatgtga cctgatatcg ggggttagtt cgtcatcatt gatgagggtt
    4381 gattatcaca gtttattact ctgaattggc tatccgcgtg tgtacctcta cctggagttt
    4441 ttcccacggt ggatatttct tcttgcgctg agcgtaagag ctatctgaca gaacagttct
    4501 tctttgcttc ctcgccagtt cgctcgctat gctcggttac acggctgcgg cgagcgctag
    4561 tgataataag tgactgaggt atgtgctctt cttatctcct tttgtagtgt tgctcttatt
    4621 ttaaacaact ttgcggtttt ttgatgactt tgcgattttg ttgttgcttt gcagtaaatt
    4681 gcaagattta ataaaaaaac gcaaagcaat gattaaagga tgttcagaat gaaactcatg
    4741 gaaacactta accagtgcat aaacgctggt catgaaatga cgaaggctat cgccattgca
    4801 cagtttaatg atgacagccc ggaagcgagg aaaataaccc ggcgctggag aataggtgaa
    4861 gcagcggatt tagttggggt ttcttctcag gctatcagag atgccgagaa agcagggcga
    4921 ctaccgcacc cggatatgga aattcgagga cgggttgagc aacgtgttgg ttatacaatt
    4981 gaacaaatta atcatatgcg tgatgtgttt ggtacgcgat tgcgacgtgc tgaagacgta
    5041 tttccaccgg tgatcggggt tgctgcccat aaaggtggcg tttacaaaac ctcagtttct
    5101 gttcatcttg ctcaggatct ggctctgaag gggctacgtg ttttgctcgt ggaaggtaac
    5161 gacccccagg gaacagcctc aatgtatcac ggatgggtac cagatcttca tattcatgca
    5221 gaagacactc tcctgccttt ctatcttggg gaaaaggacg atgtcactta tgcaataaag
    5281 cccacttgct ggccggggct tgacattatt ccttcctgtc tggctctgca ccgtattgaa
    5341 actgagttaa tgggcaaatt tgatgaaggt aaactgccca ccgatccaca cctgatgctc
    5401 cgactggcca ttgaaactgt tgctcatgac tatgatgtca tagttattga cagcgcgcct
    5461 aacctgggta tcggcacgat taatgtcgta tgtgctgctg atgtgctgat tgttcccacg
    5521 cctgctgagt tgtttgacta cacctccgca ctgcagtttt tcgatatgct tcgtgatctg
    5581 ctcaagaacg ttgatcttaa agggttcgag cctgatgtac gtattttgct taccaaatac
    5641 agcaatagca atggctctca gtccccgtgg atggaggagc aaattcggga tgcctgggga
    5701 agcatggttc taaaaaatgt tgtacgtgaa acggatgaag ttggtaaagg tcagatccgg
    5761 atgagaactg tttttgaaca ggccattgat caacgctctt caactggtgc ctggagaaat
    5821 gctctttcta tttgggaacc tgtctgcaat gaaattttcg atcgtctgat taaaccacgc
    5881 tgggagatta gataatgaag cgtgcgcctg ttattccaaa acatacgctc aatactcaac
    5941 cggttgaaga tacttcgtta tcgacaccag ctgccccgat ggtggattcg ttaattgcgc
    6001 gcgtaggagt aatggctcgc ggtaatgcca ttactttgcc tgtatgtggt cgggatgtga
    6061 agtttactct tgaagtgctc cggggtgata gtgttgagaa gacctctcgg gtatggtcag
    6121 gtaatgaacg tgaccaggag ctgcttactg aggacgcact ggatgatctc atcccttctt
    6181 ttctactgac tggtcaacag acaccggcgt tcggtcgaag agtatctggt gtcatagaaa
    6241 ttgccgatgg gagtcgccgt cgtaaagctg ctgcacttac cgaaagtgat tatcgtgttc
    6301 tggttggcga gctggatgat gagcagatgg ctgcattatc cagattgggt aacgattatc
    6361 gcccaacaag tgcttatgaa cgtggtcagc gttatgcaag ccgattgcag aatgaatttg
    6421 ctggaaatat ttctgcgctg gctgatgcgg aaaatatttc acgtaagatt attacccgct
    6481 gtatcaacac cgccaaattg cctaaatcag ttgttgctct tttttctcac cccggtgaac
    6541 tatctgcccg gtcaggtgat gcacttcaaa aagcctttac agataaagag gaattactta
    6601 agcagcaggc atctaacctt catgagcaga aaaaagctgg ggtgatattt gaagctgaag
    6661 aagttatcac tcttttaact tctgtgctta aaacgtcatc tgcatcaaga actagtttaa
    6721 gctcacgaca tcagtttgct cctggagcga cagtattgta taagggcgat aaaatggtgc
    6781 ttaacctgga caggtctcgt gttccaactg agtgtataga gaaaattgag gccattctta
    6841 aggaacttga aaagccagca ccctgatgcg accacgtttt agtctacgtt tatctgtctt
    6901 tacttaatgt cctttgttac aggccagaaa gcataactgg cctgaatatt ctctctgggc
    6961 ccactgttcc acttgtatcg tcggtctgat aatcagactg ggaccacggt cccactcgta
    7021 tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt
    7081 agtctgggac cacggtccca ctcgtatcgt cggtctgata atcagactgg gaccacggtc
    7141 ccactcgtat cgtcggtctg attattagtc tgggaccatg gtcccactcg tatcgtcggt
    7201 ctgattatta gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctgga
    7261 accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt
    7321 atcgtcggtc tgattattag tctgggacca cgatcccact cgtgttgtcg gtctgattat
    7381 cggtctggga ccacggtccc acttgtattg tcgatcagac tatcagcgtg agactacgat
    7441 tccatcaatg cctgtcaagg gcaagtattg acatgtcgtc gtaacctgta gaacggagta
    7501 acctcggtgt gcggttgtat gcctgctgtg gattgctgct gtgtcctgct tatccacaac
    7561 attttgcgca cggttatgtg gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa
    7621 ccgggctgca tccgatgcaa gtgtgtcgct gtcgacgagc tcgcgagctc ggacatgagg
    7681 ttgccccgta ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac gttaagttga
    7741 tgcagatcaa ttaatacgat acctgcgtca taattgatta tttgacgtgg tttgatggcc
    7801 tccacgcacg ttgtgatatg tagatgataa tcattatcac tttacgggtc ctttccggtg
    7861 atccgacagg ttacggggcg gcgacctcgc gggttttcgc tatttatgaa aattttccgg
    7921 tttaaggcgt ttccgttctt cttcgtcata acttaatgtt tttatttaaa ataccctctg
    7981 aaaagaaagg aaacgacagg tgctgaaagc gagctttttg gcctctgtcg tttcctttct
    8041 ctgtttttgt ccgtggaatg aacaatggaa gtccgagctc atcgctaata acttcgtata
    8101 gcatacatta tacgaagtta tattcgat


</pre>




[[Category:Plasmids]]
[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Epicentre Plasmids]]
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Latest revision as of 21:39, 24 August 2011

At a Glance

pCC1BAC, Image made with PlasMapper
Type

Cloning

Origin of Replication

single-copy F-factor, multi-copy oriV

Copy #

single copy and can be induced to 10-20 copies per cell

Markers
Link to Sequence

PubMed

Features

Copy Control BamH I, EcoR I, or Hind III Cloning Ready pCC1BAC Vectors; For oriV to function as an origin of replication, oriV requires a gene product called "trfA". Promega has genetically engineered E.coli strains to have the trfA gene under the control of a tightly-regulated inducible promoter.

Accession Number

GenBank: EU140750.1

edit table



Notes

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Epicentre

References

[back to top]


See Help:References for how to manage references in EcoliWiki.


Sequence

     
        1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
       61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccacacagat
      121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
      181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatcctc
      361 tagagtcgac ctgcaggcat gcaagcttga gtattctata gtctcaccta aatagcttgg
      421 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      481 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      541 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      601 attaatgaat cggccaacgc gaaccccttg cggccgcccg ggccgtcgac caattctcat
      661 gtttgacagc ttatcatcga atttctgcca ttcatccgct tattatcact tattcaggcg
      721 tagcaaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc
      781 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      841 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      901 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      961 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     1021 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     1081 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     1141 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     1201 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     1261 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     1321 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     1381 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     1441 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1501 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc
     1561 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
     1621 ggtatttatt cgcgataagc tcatggagcg gcgtaaccgt cgcacaggaa ggacagagaa
     1681 agcgcggatc tgggaagtga cggacagaac ggtcaggacc tggattgggg aggcggttgc
     1741 cgccgctgct gctgacggtg tgacgttctc tgttccggtc acaccacata cgttccgcca
     1801 ttcctatgcg atgcacatgc tgtatgccgg tataccgctg aaagttctgc aaagcctgat
     1861 gggacataag tccatcagtt caacggaagt ctacacgaag gtttttgcgc tggatgtggc
     1921 tgcccggcac cgggtgcagt ttgcgatgcc ggagtctgat gcggttgcga tgctgaaaca
     1981 attatcctga gaataaatgc cttggccttt atatggaaat gtggaactga gtggatatgc
     2041 tgtttttgtc tgttaaacag agaagctggc tgttatccac tgagaagcga acgaaacagt
     2101 cgggaaaatc tcccattatc gtagagatcc gcattattaa tctcaggagc ctgtgtagcg
     2161 tttataggaa gtagtgttct gtcatgatgc ctgcaagcgg taacgaaaac gatttgaata
     2221 tgccttcagg aacaatagaa atcttcgtgc ggtgttacgt tgaagtggag cggattatgt
     2281 cagcaatgga cagaacaacc taatgaacac agaaccatga tgtggtctgt ccttttacag
     2341 ccagtagtgc tcgccgcagt cgagcgacag ggcgaagccc tcgagctggt tgccctcgcc
     2401 gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt
     2461 gcgagacacc gcggccggcc gccggcgttg tggatacctc gcggaaaact tggccctcac
     2521 tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac
     2581 agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg
     2641 cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag
     2701 tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt
     2761 gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct
     2821 gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta
     2881 acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg
     2941 cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc
     3001 ggtcgagtga gcgaggaagc accagggaac agcacttata tattctgctt acacacgatg
     3061 cctgaaaaaa cttcccttgg ggttatccac ttatccacgg ggatattttt ataattattt
     3121 tttttatagt ttttagatct tcttttttag agcgccttgt aggcctttat ccatgctggt
     3181 tctagagaag gtgttgtgac aaattgccct ttcagtgtga caaatcaccc tcaaatgaca
     3241 gtcctgtctg tgacaaattg cccttaaccc tgtgacaaat tgccctcaga agaagctgtt
     3301 ttttcacaaa gttatccctg cttattgact cttttttatt tagtgtgaca atctaaaaac
     3361 ttgtcacact tcacatggat ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg
     3421 taaaaatagc ccgcgaatcg tccagtcaaa cgacctcact gaggcggcat atagtctctc
     3481 ccgggatcaa aaacgtatgc tgtatctgtt cgttgaccag atcagaaaat ctgatggcac
     3541 cctacaggaa catgacggta tctgcgagat ccatgttgct aaatatgctg aaatattcgg
     3601 attgacctct gcggaagcca gtaaggatat acggcaggca ttgaagagtt tcgcggggaa
     3661 ggaagtggtt ttttatcgcc ctgaagagga tgccggcgat gaaaaaggct atgaatcttt
     3721 tccttggttt atcaaacgtg cgcacagtcc atccagaggg ctttacagtg tacatatcaa
     3781 cccatatctc attcccttct ttatcgggtt acagaaccgg tttacgcagt ttcggcttag
     3841 tgaaacaaaa gaaatcacca atccgtatgc catgcgttta tacgaatccc tgtgtcagta
     3901 tcgtaagccg gatggctcag gcatcgtctc tctgaaaatc gactggatca tagagcgtta
     3961 ccagctgcct caaagttacc agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg
     4021 tgttaatgag atcaacagca gaactccaat gcgcctctca tacattgaga aaaagaaagg
     4081 ccgccagacg actcatatcg tattttcctt ccgcgatatc acttccatga cgacaggata
     4141 gtctgagggt tatctgtcac agatttgagg gtggttcgtc acatttgttc tgacctactg
     4201 agggtaattt gtcacagttt tgctgtttcc ttcagcctgc atggattttc tcatactttt
     4261 tgaactgtaa tttttaagga agccaaattt gagggcagtt tgtcacagtt gatttccttc
     4321 tctttccctt cgtcatgtga cctgatatcg ggggttagtt cgtcatcatt gatgagggtt
     4381 gattatcaca gtttattact ctgaattggc tatccgcgtg tgtacctcta cctggagttt
     4441 ttcccacggt ggatatttct tcttgcgctg agcgtaagag ctatctgaca gaacagttct
     4501 tctttgcttc ctcgccagtt cgctcgctat gctcggttac acggctgcgg cgagcgctag
     4561 tgataataag tgactgaggt atgtgctctt cttatctcct tttgtagtgt tgctcttatt
     4621 ttaaacaact ttgcggtttt ttgatgactt tgcgattttg ttgttgcttt gcagtaaatt
     4681 gcaagattta ataaaaaaac gcaaagcaat gattaaagga tgttcagaat gaaactcatg
     4741 gaaacactta accagtgcat aaacgctggt catgaaatga cgaaggctat cgccattgca
     4801 cagtttaatg atgacagccc ggaagcgagg aaaataaccc ggcgctggag aataggtgaa
     4861 gcagcggatt tagttggggt ttcttctcag gctatcagag atgccgagaa agcagggcga
     4921 ctaccgcacc cggatatgga aattcgagga cgggttgagc aacgtgttgg ttatacaatt
     4981 gaacaaatta atcatatgcg tgatgtgttt ggtacgcgat tgcgacgtgc tgaagacgta
     5041 tttccaccgg tgatcggggt tgctgcccat aaaggtggcg tttacaaaac ctcagtttct
     5101 gttcatcttg ctcaggatct ggctctgaag gggctacgtg ttttgctcgt ggaaggtaac
     5161 gacccccagg gaacagcctc aatgtatcac ggatgggtac cagatcttca tattcatgca
     5221 gaagacactc tcctgccttt ctatcttggg gaaaaggacg atgtcactta tgcaataaag
     5281 cccacttgct ggccggggct tgacattatt ccttcctgtc tggctctgca ccgtattgaa
     5341 actgagttaa tgggcaaatt tgatgaaggt aaactgccca ccgatccaca cctgatgctc
     5401 cgactggcca ttgaaactgt tgctcatgac tatgatgtca tagttattga cagcgcgcct
     5461 aacctgggta tcggcacgat taatgtcgta tgtgctgctg atgtgctgat tgttcccacg
     5521 cctgctgagt tgtttgacta cacctccgca ctgcagtttt tcgatatgct tcgtgatctg
     5581 ctcaagaacg ttgatcttaa agggttcgag cctgatgtac gtattttgct taccaaatac
     5641 agcaatagca atggctctca gtccccgtgg atggaggagc aaattcggga tgcctgggga
     5701 agcatggttc taaaaaatgt tgtacgtgaa acggatgaag ttggtaaagg tcagatccgg
     5761 atgagaactg tttttgaaca ggccattgat caacgctctt caactggtgc ctggagaaat
     5821 gctctttcta tttgggaacc tgtctgcaat gaaattttcg atcgtctgat taaaccacgc
     5881 tgggagatta gataatgaag cgtgcgcctg ttattccaaa acatacgctc aatactcaac
     5941 cggttgaaga tacttcgtta tcgacaccag ctgccccgat ggtggattcg ttaattgcgc
     6001 gcgtaggagt aatggctcgc ggtaatgcca ttactttgcc tgtatgtggt cgggatgtga
     6061 agtttactct tgaagtgctc cggggtgata gtgttgagaa gacctctcgg gtatggtcag
     6121 gtaatgaacg tgaccaggag ctgcttactg aggacgcact ggatgatctc atcccttctt
     6181 ttctactgac tggtcaacag acaccggcgt tcggtcgaag agtatctggt gtcatagaaa
     6241 ttgccgatgg gagtcgccgt cgtaaagctg ctgcacttac cgaaagtgat tatcgtgttc
     6301 tggttggcga gctggatgat gagcagatgg ctgcattatc cagattgggt aacgattatc
     6361 gcccaacaag tgcttatgaa cgtggtcagc gttatgcaag ccgattgcag aatgaatttg
     6421 ctggaaatat ttctgcgctg gctgatgcgg aaaatatttc acgtaagatt attacccgct
     6481 gtatcaacac cgccaaattg cctaaatcag ttgttgctct tttttctcac cccggtgaac
     6541 tatctgcccg gtcaggtgat gcacttcaaa aagcctttac agataaagag gaattactta
     6601 agcagcaggc atctaacctt catgagcaga aaaaagctgg ggtgatattt gaagctgaag
     6661 aagttatcac tcttttaact tctgtgctta aaacgtcatc tgcatcaaga actagtttaa
     6721 gctcacgaca tcagtttgct cctggagcga cagtattgta taagggcgat aaaatggtgc
     6781 ttaacctgga caggtctcgt gttccaactg agtgtataga gaaaattgag gccattctta
     6841 aggaacttga aaagccagca ccctgatgcg accacgtttt agtctacgtt tatctgtctt
     6901 tacttaatgt cctttgttac aggccagaaa gcataactgg cctgaatatt ctctctgggc
     6961 ccactgttcc acttgtatcg tcggtctgat aatcagactg ggaccacggt cccactcgta
     7021 tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt
     7081 agtctgggac cacggtccca ctcgtatcgt cggtctgata atcagactgg gaccacggtc
     7141 ccactcgtat cgtcggtctg attattagtc tgggaccatg gtcccactcg tatcgtcggt
     7201 ctgattatta gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctgga
     7261 accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt
     7321 atcgtcggtc tgattattag tctgggacca cgatcccact cgtgttgtcg gtctgattat
     7381 cggtctggga ccacggtccc acttgtattg tcgatcagac tatcagcgtg agactacgat
     7441 tccatcaatg cctgtcaagg gcaagtattg acatgtcgtc gtaacctgta gaacggagta
     7501 acctcggtgt gcggttgtat gcctgctgtg gattgctgct gtgtcctgct tatccacaac
     7561 attttgcgca cggttatgtg gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa
     7621 ccgggctgca tccgatgcaa gtgtgtcgct gtcgacgagc tcgcgagctc ggacatgagg
     7681 ttgccccgta ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac gttaagttga
     7741 tgcagatcaa ttaatacgat acctgcgtca taattgatta tttgacgtgg tttgatggcc
     7801 tccacgcacg ttgtgatatg tagatgataa tcattatcac tttacgggtc ctttccggtg
     7861 atccgacagg ttacggggcg gcgacctcgc gggttttcgc tatttatgaa aattttccgg
     7921 tttaaggcgt ttccgttctt cttcgtcata acttaatgtt tttatttaaa ataccctctg
     7981 aaaagaaagg aaacgacagg tgctgaaagc gagctttttg gcctctgtcg tttcctttct
     8041 ctgtttttgt ccgtggaatg aacaatggaa gtccgagctc atcgctaata acttcgtata
     8101 gcatacatta tacgaagtta tattcgat
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