pGEM-3Zf(-): Difference between revisions

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==At a Glance==
==At a Glance==
[[File:PGEM-3Zf(-).jpg|thumb|450px|pGEM-3Zf(-), Image made with [[PlasMapper]]]]
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="U471e09c61a3e2"  class=" tableEdit PlasmidTable" 
 
|-
|-
!{{table heading style}} |Type
!align=left {{table heading style}} |Type
|
||
Cloning
Cloning
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
|
||
Phage f1
f1
|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
|
||


|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
|
||
Beta-lactamase
Ampicillin
|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
|
||
[http://www.promega.com/vectors/pgem3zfm.txt Promega]
[http://www.ncbi.nlm.nih.gov/nuccore/8216946?report=fasta NCBI]
|-
|-
!{{table heading style}} |Comments
!align=left {{table heading style}} |Features
|
||
T7 Promoter<br />
SP6 Promoter<br />
Multiple cloning region<br />
lac operon<br />
|-
!align=left {{table heading style}} |Accession Number
||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=8216946 X65307]


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==Notes==
pGEM-3Zf(-) contains phage f1 origin of replication. Within the α-peptide coding region of β-galactosidase is included a multiple cloning site enclosed by T7 and SP6 RNA polymerase promoters on either side. Inactivation of the α-peptide through insertion allows for the simple blue/white color screening for recombinant clones. This vector can be used for standard cloning, ssDNA production, and in vitro transcription from the SP6 and T7 RNA polymerase promoters. pGEM-3Zf(-) differs from pGEM-3Zf(+) in f1 origin orientation.
==History {{HelpIcon|Help:Plasmid}}==
==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
{{PlasmidHistory}}
pGEM-3Zf(-) is derived from pGEM-3Z by the Promega Corporation.


==Sources==
==Sources==
[http://www.promega.com/catalog/CatalogProducts.asp?catalog%5Fname=Promega%5FProducts&category%5Fname=pGEM%2D3Zf%28%2B%2F%2D%29+Vectors&description%5Ftext=pGEM%3Csup%3E%26%23x00AE%3B%3C%2Fsup%3E%2D3Zf%28%2B%2F%26%23x2013%3B%29+Vectors&cookie%5Ftest=1 Promega]
[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/pgem-and-psp-subcloning-vectors/pgem_3zf_____-vectors/ Promega]


==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />


==Sequence==
==Sequence==
{{PlasmidSeq}}
<pre>
1  GGGCGAATTC GAGCTCGGTA CCCGGGGATC CTCTAGAGTC GACCTGCAGG
 
51  CATGCAAGCT TGAGTATTCT ATAGTGTCAC CTAAATAGCT TGGCGTAATC
 
101  ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC
 
151  ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
 
201  GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC
 
251  GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA
 
301  GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
 
351  CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC
 
401  GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
 
451  GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
 
501  GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
 
551  CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
 
601  TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
 
651  ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
 
701  TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
 
751  TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
 
801  GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
 
851  GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
 
901  CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA
 
951  GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
 
1001  TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
 
1051  TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
 
1101  CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
 
1151  TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
 
1201  TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
 
1251  ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
 
1301  GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
 
1351  TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
 
1401  CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
 
1451  AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
 
1501  TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
 
1551  AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
 
1601  CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
 
1651  GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
 
1701  CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
 
1751  TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
 
1801  TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
 
1851  CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
 
1901  ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
 
1951  GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
 
2001  ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
 
2051  TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
 
2101  CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
 
2151  AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
 
2201  TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
 
2251  CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT
 
2301  AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA
 
2351  AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG
 
2401  CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC
 
2451  GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG
 
2501  AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT
 
2551  ACCGCATCAG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG
 
2601  TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT
 
2651  CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG
 
2701  TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC
 
2751  GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG
 
2801  CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
 
2851  CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT
 
2901  CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG
 
2951  TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT
 
3001  ATTAACGCTT ACAATTTCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA
 
3051  AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG
 
3101  GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA
 
3151  CGACGTTGTA AAACGACGGC CAGTGAATTG TAATACGACT CACTATA




</pre>




[[Category:Plasmids]]
[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]][[Category:MCS]][[Category:lacZ]][[Category:lac operon]][[Category:beta-lactamase]][[Category:pUC/M13 primer]]
__NOTOC__
__NOTOC__

Latest revision as of 17:29, 18 August 2011

At a Glance

pGEM-3Zf(-), Image made with PlasMapper
Type

Cloning

Origin of Replication

f1

Copy #
Markers

Ampicillin

Link to Sequence

NCBI

Features

T7 Promoter
SP6 Promoter
Multiple cloning region
lac operon

Accession Number

X65307

edit table



Notes

pGEM-3Zf(-) contains phage f1 origin of replication. Within the α-peptide coding region of β-galactosidase is included a multiple cloning site enclosed by T7 and SP6 RNA polymerase promoters on either side. Inactivation of the α-peptide through insertion allows for the simple blue/white color screening for recombinant clones. This vector can be used for standard cloning, ssDNA production, and in vitro transcription from the SP6 and T7 RNA polymerase promoters. pGEM-3Zf(-) differs from pGEM-3Zf(+) in f1 origin orientation.

History

You can help EcoliWiki by writing about the history of this plasmid. pGEM-3Zf(-) is derived from pGEM-3Z by the Promega Corporation.

Sources

Promega

References

[back to top]


See Help:References for how to manage references in EcoliWiki.


Sequence

1  GGGCGAATTC GAGCTCGGTA CCCGGGGATC CTCTAGAGTC GACCTGCAGG

51  CATGCAAGCT TGAGTATTCT ATAGTGTCAC CTAAATAGCT TGGCGTAATC

101  ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC

151  ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA

201  GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC

251  GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA

301  GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG

351  CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC

401  GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT

451  GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG

501  GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG

551  CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT

601  TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT

651  ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA

701  TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC

751  TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC

801  GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT

851  GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG

901  CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA

951  GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT

1001  TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT

1051  TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT

1101  CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG

1151  TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC

1201  TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA

1251  ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC

1301  GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA

1351  TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA

1401  CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC

1451  AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA

1501  TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT

1551  AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG

1601  CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC

1651  GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT

1701  CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT

1751  TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT

1801  TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG

1851  CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC

1901  ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC

1951  GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC

2001  ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC

2051  TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG

2101  CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA

2151  AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT

2201  TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC

2251  CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT

2301  AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA

2351  AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG

2401  CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC

2451  GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG

2501  AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT

2551  ACCGCATCAG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG

2601  TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT

2651  CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG

2701  TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC

2751  GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG

2801  CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT

2851  CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT

2901  CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG

2951  TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT

3001  ATTAACGCTT ACAATTTCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA

3051  AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG

3101  GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA

3151  CGACGTTGTA AAACGACGGC CAGTGAATTG TAATACGACT CACTATA
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