pGL2-Basic: Difference between revisions

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==At a Glance==
==At a Glance==
[[File:PGL2-Basic.jpg|thumb|450px|pGL2-Basic, Image made with [[PlasMapper]]]]
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{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="D47176b06a95f8"  class=" tableEdit PlasmidTable" 
 
|-
|-
!{{table heading style}} |Type
!align=left {{table heading style}} |Type
||
Cloning
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
||
f1
|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Copy #
||
 
|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Markers
||
Ampicillin
|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Link to Sequence
||
[http://www.ncbi.nlm.nih.gov/nuccore/27650339?report=fasta NCBI]
|-
|-
!{{table heading style}} |Comments
!align=left {{table heading style}} |Features
||
SV40 Promoter<Br />
SV40 Enhancer<Br />
Multiple cloning region<Br />
GLprimer2 binding site<Br />
Luciferase gene (''luc'')<Br />
SV40 late poly(A) signal<Br />
RVprimer4 binding site<Br />
beta-lactamase (Amp res) gene<Br />
ColE1-derived plasmid replication origin<Br />
GLprimer1 binding site
|-
|-
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!align=left {{table heading style}} |Accession Number
||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27650339 X65323]
 
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==Notes==
The pGL2-Basic Vector lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences.  Expression of luciferase activity in cells transfected with this plasmid depends on insertion and proper orientation of a functional promoter upstream from luc. Potential enhancer elements can also be inserted upstream of the promoter or in the BamHI or SalI sites downstream of the luciferase gene.
==History {{HelpIcon|Help:Plasmid}}==
==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}
{{PlasmidHistory}}


==Sources==
==Sources==
{{PlasmidSources}}
[http://www.promega.com/search-results/?q=pGL2-Basic Promega]


==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
<ref name='PMID:3323819'/>
<ref name='PMID:2889129'/>
==Sequence==
 
<pre>
  1  CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTAAGTAAGC
 
51  TTGGCATTCC GGTACTGTTG GTAAAATGGA AGACGCCAAA AACATAAAGA
 
101  AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA
 
151  CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT
 
201  TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA
 
251  TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT
 
301  CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT
 
351  GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT
 
401  ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA
 
451  GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA
 
501  ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC
 
551  AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT
 
601  TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT
 
651  TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG
 
701  CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT
 
751  ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC
 
801  ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG
 
851  GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA
 
901  TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT
 
951  TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT
 
1001  TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA
 
1051  GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT
 
1101  CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC
 
1151  CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT
 
1201  CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT
 
1251  CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA
 
1301  ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC
 
1351  TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT
 
1401  TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT
 
1451  TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT
 
1501  GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC
 
1551  GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG
 
1601  CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT
 
1651  CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC
 
1701  CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG
 
1751  ACGAAATTCT TAGCTATTGT AATACTGCGA TGAGTGGCAG GGCGGGGCGT
 
1801  AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TTGCTACGCC
 
1851  TGAATAAGTG ATAATAAGCG GATGAATGGC AGAAATTCGC CGGATCTTTG
 
1901  TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA
 
1951  GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT
 
2001  TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA
 
2051  CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT
 
2101  TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA
 
2151  ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT
 
2201  TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA


==Sequence==
2251  ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT
{{PlasmidSeq}}
 
2301  ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA
 
2351  ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA
 
2401  GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT
 
2451  AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA
 
2501  GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA
 
2551  AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG
 
2601  TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
 
2651  AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG
 
2701  TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCCGTCGAC
 
2751  CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG
 
2801  GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT
 
2851  CGTAGGACAG GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
 
2901  CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC
 
2951  GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
 
3001  GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
 
3051  GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
 
3101  CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
 
3151  TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
 
3201  ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
 
3251  TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
 
3301  TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
 
3351  GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
 
3401  GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
 
3451  CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA
 
3501  GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
 
3551  TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
 
3601  TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
 
3651  CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
 
3701  TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
 
3751  TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
 
3801  ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
 
3851  GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
 
3901  TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
 
3951  CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
 
4001  AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
 
4051  TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
 
4101  AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
 
4151  CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
 
4201  GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
 
4251  CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
 
4301  TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
 
4351  TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
 
4401  CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
 
4451  ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
 
4501  GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
 
4551  ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
 
4601  TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
 
4651  CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
 
4701  AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
 
4751  TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
 
4801  CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT
 
4851  ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT
 
4901  CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG
 
4951  CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC
 
5001  CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC
 
5051  GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA
 
5101  ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC
 
5151  TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA
 
5201  AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA
 
5251  CGCTTACAAT TTGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG
 
5301  CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCCCAAGC TACCATGATA
 
5351  AGTAAGTAAT ATTAAGGTAC GTGGAGGTTT TACTTGCTTT AAAAAACCTC
 
5401  CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT
 
5451  TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
 
5501  CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG
 
5551  TCCAAACTCA TCAATGTATC TTATGGTACT GTAACTGAGC TAACATAA


</pre>






[[Category:Plasmids]]
[[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]]
__NOTOC__
__NOTOC__

Latest revision as of 21:36, 26 August 2011

At a Glance

pGL2-Basic, Image made with PlasMapper
Type

Cloning

Origin of Replication

f1

Copy #
Markers

Ampicillin

Link to Sequence

NCBI

Features

SV40 Promoter
SV40 Enhancer
Multiple cloning region
GLprimer2 binding site
Luciferase gene (luc)
SV40 late poly(A) signal
RVprimer4 binding site
beta-lactamase (Amp res) gene
ColE1-derived plasmid replication origin
GLprimer1 binding site

Accession Number

X65323

edit table



Notes

The pGL2-Basic Vector lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences. Expression of luciferase activity in cells transfected with this plasmid depends on insertion and proper orientation of a functional promoter upstream from luc. Potential enhancer elements can also be inserted upstream of the promoter or in the BamHI or SalI sites downstream of the luciferase gene.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Promega

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

[1] [2]

Sequence

  1  CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTAAGTAAGC

 51  TTGGCATTCC GGTACTGTTG GTAAAATGGA AGACGCCAAA AACATAAAGA

101  AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA

151  CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT

201  TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA

251  TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT

301  CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT

351  GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT

401  ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA

451  GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA

501  ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC

551  AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT

601  TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT

651  TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG

701  CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT

751  ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC

801  ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG

851  GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA

901  TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT

951  TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT

1001  TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA

1051  GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT

1101  CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC

1151  CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT

1201  CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT

1251  CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA

1301  ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC

1351  TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT

1401  TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT

1451  TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT

1501  GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC

1551  GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG

1601  CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT

1651  CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC

1701  CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG

1751  ACGAAATTCT TAGCTATTGT AATACTGCGA TGAGTGGCAG GGCGGGGCGT

1801  AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TTGCTACGCC

1851  TGAATAAGTG ATAATAAGCG GATGAATGGC AGAAATTCGC CGGATCTTTG

1901  TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA

1951  GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT

2001  TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA

2051  CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT

2101  TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA

2151  ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT

2201  TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA

2251  ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT

2301  ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA

2351  ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA

2401  GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT

2451  AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA

2501  GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA

2551  AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG

2601  TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT

2651  AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG

2701  TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCCGTCGAC

2751  CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG

2801  GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT

2851  CGTAGGACAG GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT CACTGACTCG

2901  CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC

2951  GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT

3001  GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG

3051  GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG

3101  CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT

3151  TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT

3201  ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA

3251  TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC

3301  TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC

3351  GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT

3401  GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG

3451  CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA

3501  GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT

3551  TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT

3601  TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT

3651  CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG

3701  TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC

3751  TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA

3801  ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC

3851  GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA

3901  TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA

3951  CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC

4001  AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA

4051  TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT

4101  AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG

4151  CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC

4201  GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT

4251  CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT

4301  TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT

4351  TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG

4401  CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC

4451  ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC

4501  GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC

4551  ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC

4601  TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG

4651  CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA

4701  AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT

4751  TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC

4801  CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT

4851  ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT

4901  CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG

4951  CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC

5001  CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC

5051  GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA

5101  ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC

5151  TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA

5201  AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA

5251  CGCTTACAAT TTGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG

5301  CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCCCAAGC TACCATGATA

5351  AGTAAGTAAT ATTAAGGTAC GTGGAGGTTT TACTTGCTTT AAAAAACCTC

5401  CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT

5451  TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA

5501  CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG

5551  TCCAAACTCA TCAATGTATC TTATGGTACT GTAACTGAGC TAACATAA
  1. ↑ Henikoff, S (1987) Unidirectional digestion with exonuclease III in DNA sequence analysis. Meth. Enzymol. 155 156-65 PubMed
  2. ↑ Rosenthal, N (1987) Identification of regulatory elements of cloned genes with functional assays. Meth. Enzymol. 152 704-20 PubMed
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