pGL2-Basic
At a Glance
pGL2-Basic, Image made with PlasMapper
<protect>
| Type |
Cloning |
|---|---|
| Origin of Replication |
f1 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
SV40 Promoter |
| Accession Number | |
| edit table |
</protect>
Notes
The pGL2-Basic Vector lacks eukaryotic promoter and enhancer sequences, allowing maximum flexibility in cloning putative regulatory sequences. Expression of luciferase activity in cells transfected with this plasmid depends on insertion and proper orientation of a functional promoter upstream from luc. Potential enhancer elements can also be inserted upstream of the promoter or in the BamHI or SalI sites downstream of the luciferase gene.
History
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Sources
References
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Sequence
1 CCCGGGAGGT ACCGAGCTCT TACGCGTGCT AGCTCGAGAT CTAAGTAAGC 51 TTGGCATTCC GGTACTGTTG GTAAAATGGA AGACGCCAAA AACATAAAGA 101 AAGGCCCGGC GCCATTCTAT CCTCTAGAGG ATGGAACCGC TGGAGAGCAA 151 CTGCATAAGG CTATGAAGAG ATACGCCCTG GTTCCTGGAA CAATTGCTTT 201 TACAGATGCA CATATCGAGG TGAACATCAC GTACGCGGAA TACTTCGAAA 251 TGTCCGTTCG GTTGGCAGAA GCTATGAAAC GATATGGGCT GAATACAAAT 301 CACAGAATCG TCGTATGCAG TGAAAACTCT CTTCAATTCT TTATGCCGGT 351 GTTGGGCGCG TTATTTATCG GAGTTGCAGT TGCGCCCGCG AACGACATTT 401 ATAATGAACG TGAATTGCTC AACAGTATGA ACATTTCGCA GCCTACCGTA 451 GTGTTTGTTT CCAAAAAGGG GTTGCAAAAA ATTTTGAACG TGCAAAAAAA 501 ATTACCAATA ATCCAGAAAA TTATTATCAT GGATTCTAAA ACGGATTACC 551 AGGGATTTCA GTCGATGTAC ACGTTCGTCA CATCTCATCT ACCTCCCGGT 601 TTTAATGAAT ACGATTTTGT ACCAGAGTCC TTTGATCGTG ACAAAACAAT 651 TGCACTGATA ATGAATTCCT CTGGATCTAC TGGGTTACCT AAGGGTGTGG 701 CCCTTCCGCA TAGAACTGCC TGCGTCAGAT TCTCGCATGC CAGAGATCCT 751 ATTTTTGGCA ATCAAATCAT TCCGGATACT GCGATTTTAA GTGTTGTTCC 801 ATTCCATCAC GGTTTTGGAA TGTTTACTAC ACTCGGATAT TTGATATGTG 851 GATTTCGAGT CGTCTTAATG TATAGATTTG AAGAAGAGCT GTTTTTACGA 901 TCCCTTCAGG ATTACAAAAT TCAAAGTGCG TTGCTAGTAC CAACCCTATT 951 TTCATTCTTC GCCAAAAGCA CTCTGATTGA CAAATACGAT TTATCTAATT 1001 TACACGAAAT TGCTTCTGGG GGCGCACCTC TTTCGAAAGA AGTCGGGGAA 1051 GCGGTTGCAA AACGCTTCCA TCTTCCAGGG ATACGACAAG GATATGGGCT 1101 CACTGAGACT ACATCAGCTA TTCTGATTAC ACCCGAGGGG GATGATAAAC 1151 CGGGCGCGGT CGGTAAAGTT GTTCCATTTT TTGAAGCGAA GGTTGTGGAT 1201 CTGGATACCG GGAAAACGCT GGGCGTTAAT CAGAGAGGCG AATTATGTGT 1251 CAGAGGACCT ATGATTATGT CCGGTTATGT AAACAATCCG GAAGCGACCA 1301 ACGCCTTGAT TGACAAGGAT GGATGGCTAC ATTCTGGAGA CATAGCTTAC 1351 TGGGACGAAG ACGAACACTT CTTCATAGTT GACCGCTTGA AGTCTTTAAT 1401 TAAATACAAA GGATATCAGG TGGCCCCCGC TGAATTGGAA TCGATATTGT 1451 TACAACACCC CAACATCTTC GACGCGGGCG TGGCAGGTCT TCCCGACGAT 1501 GACGCCGGTG AACTTCCCGC CGCCGTTGTT GTTTTGGAGC ACGGAAAGAC 1551 GATGACGGAA AAAGAGATCG TGGATTACGT CGCCAGTCAA GTAACAACCG 1601 CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG TGGACGAAGT ACCGAAAGGT 1651 CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC TCATAAAGGC 1701 CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG 1751 ACGAAATTCT TAGCTATTGT AATACTGCGA TGAGTGGCAG GGCGGGGCGT 1801 AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TTGCTACGCC 1851 TGAATAAGTG ATAATAAGCG GATGAATGGC AGAAATTCGC CGGATCTTTG 1901 TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA ACTACCTACA 1951 GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT 2001 TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA 2051 CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT 2101 TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA 2151 ACATTCTACT CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT 2201 TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT TAGTAATAGA 2251 ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT 2301 ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA 2351 ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA 2401 GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT 2451 AATTTGTAAA GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA 2501 GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA 2551 AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG 2601 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 2651 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG 2701 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCCGTCGAC 2751 CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG 2801 GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT 2851 CGTAGGACAG GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT CACTGACTCG 2901 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 2951 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 3001 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 3051 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 3101 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 3151 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 3201 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 3251 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 3301 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 3351 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 3401 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 3451 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA 3501 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 3551 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 3601 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 3651 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 3701 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 3751 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 3801 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC 3851 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 3901 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 3951 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 4001 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 4051 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 4101 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG 4151 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 4201 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 4251 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 4301 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 4351 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 4401 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC 4451 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 4501 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 4551 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 4601 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 4651 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA 4701 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT 4751 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 4801 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 4851 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 4901 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 4951 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 5001 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC 5051 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 5101 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 5151 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 5201 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA 5251 CGCTTACAAT TTGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG 5301 CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCCCAAGC TACCATGATA 5351 AGTAAGTAAT ATTAAGGTAC GTGGAGGTTT TACTTGCTTT AAAAAACCTC 5401 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT 5451 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 5501 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG 5551 TCCAAACTCA TCAATGTATC TTATGGTACT GTAACTGAGC TAACATAA
