pGEMEX-2: Difference between revisions

Latest revision as of 19:29, 18 August 2011

At a Glance

Type	Expression
Origin of Replication	f1
Copy #
Markers	Ampicillin
Link to Sequence	NCBI
Features	Multiple cloning region T3 RNA polymerase promoter T7 RNA polymerase promoter SP6 RNA polymerase promoter HindIII, NsiI, SphI, NotI, ApaI, XhoI, BamHI, SalI, EcoRI, SacI, SfiI
Accession Number	X65318
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Notes

The addition of two cytosine bases at positions 65 and 84 between pGEMEX-1 and pGEMEX-2.Special usage restrictions

History

Designed for cloning, as templates for in vitro transcription and for production of ssDNA.

Sources

Promega. Discontinued.

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

Sequence

       1 gaatactcaa gcttatgcat gcggccgcat ctagagggcc cggatccctc gaggtcgacg
      61 aattccgagc tcggccgact tggcccttcc ctttagtgag ggttaataaa cttggccaat
     121 tcgatccggt accagcacca ccagcggtga ggtgcggaac ttctacaacc tcaaagccca
     181 taacgttgcg gatagaaccc ttctcagggt caatcagagc agcgtagttt gctgcgttcg
     241 gcatcagtgc tgccagaatc gcagagtagc tatctgggtc acagtagaac acacggtcag
     301 cagccggaac atagttcttg gtcagagccg cacgagcctt agtcagagcc gcaataatct
     361 ccttacccag cgcaacttgg tcggtaagtg cggccttgtt ctgagtggtc tcaattacgg
     421 tagcagtacc taagccctcg atgttctcat tatatttgct ttccacgtta cacagaccgg
     481 caatctcagc cagaaccgca ccatccgcag ccatcgccag agattcaccc aactgagagg
     541 tatactcaga gcgaacgtcg tagtggttca tcgcgtcctc aatatcataa atcagaacgt
     601 cagccgtcag gagaccgtca atggtgatta ccttctcggt gtgtttgatg tccttacgtt
     661 tatcgtcgag gttctcgccc ggagccagat acgctgcctg agtgcgaccc agaacaggga
     721 actgagcgga tttaccgctg gagatggaac gtaccatgtg gcgagaagtg gtcacggagg
     781 tacgagcgaa cgcagtcagg acttcaccgc caaatacctt caagaacaac gccagtttat
     841 ctccagcagc aactacacct ttaccttggt tagtacccat ttgctgtcca ccagtcatgc
     901 tagccatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt
     961 gtggtctccc tatagtgagt cgtattaatt tcgcgggatc gagatctccc gggctgcatt
    1021 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    1081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    1141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    1201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    1261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    1321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    1381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    1441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    1501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    1561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    1621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    1681 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    1741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    1801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    1861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    1921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    1981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    2041 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    2101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    2161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    2221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    2281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    2341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    2401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    2461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    2521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    2581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    2641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    2701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    2761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    2821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    2881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    2941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    3001 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    3061 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    3121 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    3181 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    3241 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    3301 tcaggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    3361 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    3421 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    3481 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    3541 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    3601 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
    3661 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
    3721 atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg
    3781 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca ggttatccgg
    3841 atatagttcc tcctttcagc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt
    3901 atgctagtta ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag
    3961 cagccggatc aagctgggat ttaggtgaca ctata

@@ Line 1: / Line 1: @@
-==At a Glance {{HelpIcon|Help:Plasmid}}==
+==At a Glance ==
+[[Image:PGEMEX-2.jpg|thumb|450px|right|pGEMEX-2, Image provided by [http://www.promega.com/figures/0300/0311vbw2.gif Promega]]]
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+{| {{Prettytable}} width='30%' align=left  id="M4712e9fc7f80d"  class=" tableEdit PlasmidTable"
 |-
-!{{table heading style}} |Type
+!align=left {{table heading style}} |Type
+||
+Expression
 |-
-!{{table heading style}} |Origin of Replication
+!align=left {{table heading style}} |Origin of Replication
+||
+f1
 |-
-!{{table heading style}} |Copy #
+!align=left {{table heading style}} |Copy #
+||
 |-
-!{{table heading style}} |Markers
+!align=left {{table heading style}} |Markers
+||
+Ampicillin
 |-
-!{{table heading style}} |Link to Sequence
+!align=left {{table heading style}} |Link to Sequence
+||
+[http://www.ncbi.nlm.nih.gov/nuccore/8216954?report=fasta NCBI]
 |-
-!{{table heading style}} |Comments
+!align=left {{table heading style}} |Features
+||
+Multiple cloning region<br />
+T3 RNA polymerase promoter <br />
+T7 RNA polymerase promoter <br />
+SP6 RNA polymerase promoter<br />
+HindIII, NsiI,  SphI, NotI,  ApaI, XhoI,  BamHI,  SalI,  EcoRI,  SacI,  SfiI
 |-
-|colspan='1'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.M4712e9fc7f80d&page=0&pagename={{FULLPAGENAMEE}} edit table]
+!align=left {{table heading style}} |Accession Number
+||
+[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=8216954 X65318]
+|- class="tableEdit_footer"
+|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.M4712e9fc7f80d&page=113006&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> ||
 |}
 <!--box uid=63ce1649aab8b3180235b0af7618f667.0.M4712e9fc7f80d--></protect>
 <BR>
 <Br clear='all'>
-==History {{HelpIcon|Help:Plasmid}}==
-{{PlasmidHistory}}
-==Sources {{HelpIcon|Help:Plasmid}}==
+==Notes==
-{{PlasmidSources}}
+The addition of two cytosine bases at positions 65 and 84 between pGEMEX-1 and pGEMEX-2.Special usage restrictions
+==History==
+Designed for cloning, as templates for in vitro transcription and for production of ssDNA.
+==Sources==
+[http://www.promega.com/products/pm/discontinued-vectors/ Promega]. Discontinued.
 ==References==
+{{BacktoTop}}
 {{RefHelp}}
-<references/>
+<references />
-==Sequence {{HelpIcon|Help:Plasmid}}==
+==Sequence ==
-{{PlasmidSeq}}
+gaatactcaa gcttatgcat gcggccgcat ctagagggcc cggatccctc gaggtcgacg
+aattccgagc tcggccgact tggcccttcc ctttagtgag ggttaataaa cttggccaat
+tcgatccggt accagcacca ccagcggtga ggtgcggaac ttctacaacc tcaaagccca
+taacgttgcg gatagaaccc ttctcagggt caatcagagc agcgtagttt gctgcgttcg
+gcatcagtgc tgccagaatc gcagagtagc tatctgggtc acagtagaac acacggtcag
+cagccggaac atagttcttg gtcagagccg cacgagcctt agtcagagcc gcaataatct
+ccttacccag cgcaacttgg tcggtaagtg cggccttgtt ctgagtggtc tcaattacgg
+tagcagtacc taagccctcg atgttctcat tatatttgct ttccacgtta cacagaccgg
+caatctcagc cagaaccgca ccatccgcag ccatcgccag agattcaccc aactgagagg
+tatactcaga gcgaacgtcg tagtggttca tcgcgtcctc aatatcataa atcagaacgt
+cagccgtcag gagaccgtca atggtgatta ccttctcggt gtgtttgatg tccttacgtt
+tatcgtcgag gttctcgccc ggagccagat acgctgcctg agtgcgaccc agaacaggga
+actgagcgga tttaccgctg gagatggaac gtaccatgtg gcgagaagtg gtcacggagg
+tacgagcgaa cgcagtcagg acttcaccgc caaatacctt caagaacaac gccagtttat
+ctccagcagc aactacacct ttaccttggt tagtacccat ttgctgtcca ccagtcatgc
+tagccatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt
+gtggtctccc tatagtgagt cgtattaatt tcgcgggatc gagatctccc gggctgcatt
+aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
+cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
+aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
+aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
+tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
+caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
+cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
+ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
+gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
+agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
+gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
+acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
+gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
+gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
+cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
+caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
+gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
+cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
+cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
+caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
+gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
+gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
+cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
+catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
+gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
+ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
+gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
+cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
+tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
+gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
+atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
+ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
+gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
+acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
+cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
+agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
+cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
+tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
+tcaggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
+gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
+cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
+atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
+tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
+tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
+tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
+atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg
+cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca ggttatccgg
+atatagttcc tcctttcagc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt
+atgctagtta ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag
+cagccggatc aagctgggat ttaggtgaca ctata
-[[Category:Plasmids]]
+[[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]][[Category:T3 Promoter]]
 __NOTOC__