pGEMEX-2
At a Glance
pGEMEX-2, Image provided by Promega
<protect>
| Type |
Expression |
|---|---|
| Origin of Replication |
f1 |
| Copy # | |
| Markers |
Ampicillin |
| Link to Sequence | |
| Features |
Multiple cloning region |
| Accession Number | |
| edit table |
</protect>
Notes
The addition of two cytosine bases at positions 65 and 84 between pGEMEX-1 and pGEMEX-2.Special usage restrictions
History
Designed for cloning, as templates for in vitro transcription and for production of ssDNA.
Sources
Promega. Discontinued.
References
See Help:References for how to manage references in EcoliWiki.
Sequence
1 gaatactcaa gcttatgcat gcggccgcat ctagagggcc cggatccctc gaggtcgacg
61 aattccgagc tcggccgact tggcccttcc ctttagtgag ggttaataaa cttggccaat
121 tcgatccggt accagcacca ccagcggtga ggtgcggaac ttctacaacc tcaaagccca
181 taacgttgcg gatagaaccc ttctcagggt caatcagagc agcgtagttt gctgcgttcg
241 gcatcagtgc tgccagaatc gcagagtagc tatctgggtc acagtagaac acacggtcag
301 cagccggaac atagttcttg gtcagagccg cacgagcctt agtcagagcc gcaataatct
361 ccttacccag cgcaacttgg tcggtaagtg cggccttgtt ctgagtggtc tcaattacgg
421 tagcagtacc taagccctcg atgttctcat tatatttgct ttccacgtta cacagaccgg
481 caatctcagc cagaaccgca ccatccgcag ccatcgccag agattcaccc aactgagagg
541 tatactcaga gcgaacgtcg tagtggttca tcgcgtcctc aatatcataa atcagaacgt
601 cagccgtcag gagaccgtca atggtgatta ccttctcggt gtgtttgatg tccttacgtt
661 tatcgtcgag gttctcgccc ggagccagat acgctgcctg agtgcgaccc agaacaggga
721 actgagcgga tttaccgctg gagatggaac gtaccatgtg gcgagaagtg gtcacggagg
781 tacgagcgaa cgcagtcagg acttcaccgc caaatacctt caagaacaac gccagtttat
841 ctccagcagc aactacacct ttaccttggt tagtacccat ttgctgtcca ccagtcatgc
901 tagccatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt
961 gtggtctccc tatagtgagt cgtattaatt tcgcgggatc gagatctccc gggctgcatt
1021 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
1081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
1141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
1201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
1261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
1321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
1381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
1441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
1501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
1561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
1621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
1681 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
1741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
1801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
1861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
1921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
1981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
2041 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
2101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
2161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
2221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
2281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
2341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
2401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
2461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
2521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
2581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
2641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
2701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
2761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
2821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
2881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
2941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
3001 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
3061 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
3121 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
3181 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
3241 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
3301 tcaggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
3361 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
3421 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
3481 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
3541 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
3601 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
3661 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
3721 atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg
3781 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca ggttatccgg
3841 atatagttcc tcctttcagc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt
3901 atgctagtta ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag
3961 cagccggatc aagctgggat ttaggtgaca ctata
