pET-9c: Difference between revisions

Latest revision as of 22:29, 1 September 2011

At a Glance

Type	Cloning/Expression
Origin of Replication	pBR322
Copy #	~40
Markers	Kanamycin
Link to Sequence	Novagen
Features	T7 Promoter T7 Transcription start T7•Tag MCS T7 Terminator
Accession Number
edit table

Notes

pET-9c is the same as pET-9a with the exception that pET-9b is 2bp shorter at 4339bp; for the sites beyond BamH1 at 510 subtract 2bp.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Novagen
Promega

References

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See Help:References for how to manage references in EcoliWiki.

Sequence

       1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
      61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
     121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
     181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
     241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
     301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
     361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 
     421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 
     481 cagcttcctt tcgggctttg ttagcagccg gatccgaccc atttgctgtc caccagtcat 
     541 gctagccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg 
     601 ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac 
     661 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc 
     721 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc 
     781 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat 
     841 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta 
     901 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc 
     961 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt 
    1021 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc 
    1081 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc 
    1141 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt 
    1201 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc 
    1261 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg 
    1321 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc 
    1381 gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc 
    1441 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc 
    1501 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc 
    1561 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga 
    1621 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca 
    1681 gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg 
    1741 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg 
    1801 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag 
    1861 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag 
    1921 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa 
    1981 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc 
    2041 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat 
    2101 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga 
    2161 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca 
    2221 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg 
    2281 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca 
    2341 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 
    2401 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 
    2461 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 
    2521 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg 
    2581 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc 
    2641 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
    2701 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
    2761 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
    2821 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
    2881 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
    2941 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
    3001 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
    3061 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
    3121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
    3181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
    3241 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
    3301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
    3361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
    3421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaacaat 
    3481 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga 
    3541 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa 
    3601 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc 
    3661 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 
    3721 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 
    3781 tatccgtact cctgatgatg catggttact caccactgcg atccccggga aaacagcatt 
    3841 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 
    3901 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt 
    3961 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga 
    4021 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc 
    4081 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 
    4141 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca 
    4201 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct 
    4261 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct 
    4321 cgatgagttt ttctaagaa

@@ Line 1: / Line 1: @@
 ==At a Glance==
+[[File:PET-9c.jpg|thumb|450px|pET-9c Image made with [[PlasMapper]]]]
 <protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z46fd692105957-->
 <!--
 ******************************************************************************************
 *
 *   ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
 *
 ****************************************************************************************** -->
-{|{{Prettytable}} width='30%' align=left
+{| {{Prettytable}} width='30%' align=left  id="Z46fd692105957"  class=" tableEdit PlasmidTable"
 |-
-!{{table heading style}} |Type
+!align=left {{table heading style}} |Type
-|
+||
-Expression
+Cloning/Expression
 |-
-!{{table heading style}} |Origin of Replication
+!align=left {{table heading style}} |Origin of Replication
-|
+||
-Origin	pBR322
+pBR322
 |-
-!{{table heading style}} |Copy #
+!align=left {{table heading style}} |Copy #
-|
+||
+~40
 |-
-!{{table heading style}} |Markers
+!align=left {{table heading style}} |Markers
-|
+||
+Kanamycin
 |-
-!{{table heading style}} |Link to Sequence
+!align=left {{table heading style}} |Link to Sequence
-|
+||
-[http://www.emdbiosciences.com/docs/NDIS/69433-000.html Novagen]
+[http://www.emdbiosciences.com/usa/life-science-research/vector-table-pet-9c-vector-sequence/c__Iyb.s1O8VwAAAEhZD0LddSR?WFSimpleSearch_NameOrID=pET-9c+sequence&BackButtonText=search+results Novagen]
 |-
-!{{table heading style}} |Comments
+!align=left {{table heading style}} |Features
-|
+||
-The pET-9a-d(+) vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. These vectors are the precursors to many pET family vectors. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map.
+T7 Promoter <br />
+T7 Transcription start <br />
+T7•Tag <br />
+MCS <br />
+T7 Terminator <br />
 |-
-|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Z46fd692105957&page=0&pagename={{FULLPAGENAMEE}} edit table]
+!align=left {{table heading style}} |Accession Number
+||
+|- class="tableEdit_footer"
+|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Z46fd692105957&page=112667&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> ||
 |}
 <!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z46fd692105957--></protect>
 <BR>
 <Br clear='all'>
+==Notes==
+pET-9c is the same as pET-9a with the exception that pET-9b is 2bp shorter at 4339bp; for the sites beyond BamH1 at 510 subtract 2bp.
 ==History {{HelpIcon|Help:Plasmid}}==
 {{PlasmidHistory}}
 ==Sources==
-[http://www.emdbiosciences.com/product/69433 Novagen]
+[http://www.emdbiosciences.com/product/69433 Novagen]<br />
+[http://www.promega.com/products/pm/discontinued-vectors/#DV7 Promega]
 ==References==
+{{BacktoTop}}
 {{RefHelp}}
-<references/>
+<references />
 ==Sequence==
-{{PlasmidSeq}}
+ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
+ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
+caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
+gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
+tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
+ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
+cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
+cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
+cagcttcctt tcgggctttg ttagcagccg gatccgaccc atttgctgtc caccagtcat
+gctagccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg
+ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
+gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
+gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
+ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
+gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
+atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc
+agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
+atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
+tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
+ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
+atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
+tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
+caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
+gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc
+gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
+tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
+ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
+gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
+gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
+tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
+aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
+caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
+cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
+cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
+gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
+catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
+acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca
+tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
+cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
+gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
+cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
+cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
+atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg
+tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
+tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
+aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
+aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
+ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
+acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
+ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
+tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
+tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
+gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
+agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
+tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
+agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
+tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
+acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaacaat
+aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
+aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
+atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
+cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
+tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
+tatccgtact cctgatgatg catggttact caccactgcg atccccggga aaacagcatt
+ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
+cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
+tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
+tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
+attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
+cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
+ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
+ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
+cgatgagttt ttctaagaa
+[[Category:Plasmids]][[Category:Cloning]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Promega Plasmids]][[Category:Kanamycin Marker Plasmids]][[Category:T7 Promoter]][[Category:T7 Tag]][[Category:MCS]]
-[[Category:Plasmids]]
 __NOTOC__