pET-9c
At a Glance
pET-9c Image made with PlasMapper
<protect>
| Type |
Cloning/Expression |
|---|---|
| Origin of Replication |
pBR322 |
| Copy # |
~40 |
| Markers |
Kanamycin |
| Link to Sequence | |
| Features |
T7 Promoter |
| Accession Number |
|
| edit table |
</protect>
Notes
pET-9c is the same as pET-9a with the exception that pET-9b is 2bp shorter at 4339bp; for the sites beyond BamH1 at 510 subtract 2bp.
History
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Sources
References
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Sequence
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatccgaccc atttgctgtc caccagtcat
541 gctagccata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg
601 ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
661 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
721 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
781 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
841 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
901 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc
961 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
1021 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
1081 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
1141 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
1201 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
1261 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
1321 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
1381 gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc
1441 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
1501 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
1561 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
1621 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
1681 gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
1741 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
1801 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
1861 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
1921 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
1981 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
2041 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
2101 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
2161 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca
2221 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
2281 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
2341 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
2401 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
2461 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
2521 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg
2581 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
2641 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
2701 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
2761 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
2821 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
2881 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
2941 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
3001 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
3061 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
3121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
3181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
3241 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
3301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
3361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
3421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaacaat
3481 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
3541 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
3601 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
3661 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
3721 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
3781 tatccgtact cctgatgatg catggttact caccactgcg atccccggga aaacagcatt
3841 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
3901 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
3961 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
4021 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
4081 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
4141 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
4201 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
4261 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
4321 cgatgagttt ttctaagaa
