pMAL-pIII: Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
← Older edit
Charner (talk | contribs)
student
366 edits
/Volumes/r3/t3/ul/working/touchTables/touch_all_tables.php
 
(33 intermediate revisions by 4 users not shown)
Line 1: Line 1:
==At a Glance {{HelpIcon|Help:Plasmid}}==
==At a Glance==
[[Image:PMAL-pIII.gif|thumb|400px|right|Image of pMAL-pIII.]]
[[Image:PMAL-pIII.jpg|thumb|370px|right|pMAL-pIII Image provided by [http://www.neb.com/nebecomm/products/productN8101.asp NEB]]]
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.J46e2d6a88bcca-->
<protect><!--box uid=63ce1649aab8b3180235b0af7618f667.0.J46e2d6a88bcca-->
<!--
<!--
******************************************************************************************
******************************************************************************************
*
*  
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **  
*
*  
****************************************************************************************** -->
****************************************************************************************** -->
{|{{Prettytable}} width='30%' align=left
{| {{Prettytable}} width='30%' align=left id="J46e2d6a88bcca"  class=" tableEdit PlasmidTable" 
 
|-
|-
!{{table heading style}} |Type
!align=left {{table heading style}} |Type
|
||
Cloning
Cloning
|-
|-
!{{table heading style}} |Origin of Replication
!align=left {{table heading style}} |Origin of Replication
|
||
M13, pMB1
|-
!align=left {{table heading style}} |Copy #
||


|-
|-
!{{table heading style}} |Copy #
!align=left {{table heading style}} |Markers
|
||
 
Ampicillin
|-
!align=left {{table heading style}} |Link to Sequence
||
[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/pmalpiii.txt NEB]
|-
|-
!{{table heading style}} |Markers
!align=left {{table heading style}} |Features
|
||
 
lacIq Promoter<br>
Ptac Promoter<br>
MCS
|-
|-
!{{table heading style}} |Link to Sequence
!align=left {{table heading style}} |Accession Number
|
||


|-
!{{table heading style}} |Comments
|


|-
|- class="tableEdit_footer"
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J46e2d6a88bcca&page=0&pagename={{FULLPAGENAMEE}} edit table]
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J46e2d6a88bcca&page=0&pagename={{FULLPAGENAMEE}}&type=1&template=PlasmidTable edit table]</span> ||
|}
|}
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.J46e2d6a88bcca--></protect>
<!--box uid=63ce1649aab8b3180235b0af7618f667.0.J46e2d6a88bcca--></protect>
<BR>
<BR>
<Br clear='all'>
<Br clear='all'>
==Notes==
Isolated from E. coli strain ER2272 by standard DNA purification procedure.  Rapid characterization of selected sequences by ELISA Alternative to chemical peptide synthesis Monovalency of MBP fusion allows accurate KD determination.


==History {{HelpIcon|Help:Plasmid}}==
==History==
{{PlasmidHistory}}
pMal-pIII is a derivative of pMal-p2.  Kpn1/Acc651 and EagI sites have been introduced within the pIII leader to ease direct transfer of sequences selected from any of the PhD phage display peptide libraries into an expression vector.


==Sources {{HelpIcon|Help:Plasmid}}==
==Sources==
{{PlasmidSources}}
[http://www.neb.com/nebecomm/products/productN8101.asp New England Biolabs]


==References==
==References==
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
 
==Sequence==


==Sequence {{HelpIcon|Help:Plasmid}}==
      1  CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC
{{PlasmidSeq}}
      51  CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA
    101  CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG
    151  TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG
    201  GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC
    251  GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC
    301  ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG
    351  GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG
    401  TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA
    451  TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC
    501  ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA
    551  CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC
    601  TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT
    651  TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG
    701  CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT
    751  CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT
    801  GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT
    851  TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG
    901  ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA
    951  CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT
    1001  CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG
    1051  TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC
    1101  GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA
    1151  AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG
    1201  GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT
    1251  GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC
    1301  GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT
    1351  AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG
    1401  AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG
    1451  GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA
    1501  GCACTTCACC AACAAGGACC ATAGCATATG AAAAAATTAT TATTCGCAAT
    1551  TCCTTTAGTG GTACCTTTCT ATTCTCACTC GGCCGATATG AAAATCGAAG
    1601  AAGGTAAACT GGTAATCTGG ATTAACGGCG ATAAAGGCTA TAACGGTCTC
    1651  GCTGAAGTCG GTAAGAAATT CGAGAAAGAT ACCGGAATTA AAGTCACCGT
    1701  TGAGCATCCG GATAAACTGG AAGAGAAATT CCCACAGGTT GCGGCAACTG
    1751  GCGATGGCCC TGACATTATC TTCTGGGCAC ACGACCGCTT TGGTGGCTAC
    1801  GCTCAATCTG GCCTGTTGGC TGAAATCACC CCGGACAAAG CGTTCCAGGA
    1851  CAAGCTGTAT CCGTTTACCT GGGATGCCGT ACGTTACAAC GGCAAGCTGA
    1901  TTGCTTACCC GATCGCTGTT GAAGCGTTAT CGCTGATTTA TAACAAAGAT
    1951  CTGCTGCCGA ACCCGCCAAA AACCTGGGAA GAGATCCCGG CGCTGGATAA
    2001  AGAACTGAAA GCGAAAGGTA AGAGCGCGCT GATGTTCAAC CTGCAAGAAC
    2051  CGTACTTCAC CTGGCCGCTG ATTGCTGCTG ACGGGGGTTA TGCGTTCAAG
    2101  TATGAAAACG GCAAGTACGA CATTAAAGAC GTGGGCGTGG ATAACGCTGG
    2151  CGCGAAAGCG GGTCTGACCT TCCTGGTTGA CCTGATTAAA AACAAACACA
    2201  TGAATGCAGA CACCGATTAC TCCATCGCAG AAGCTGCCTT TAATAAAGGC
    2251  GAAACAGCGA TGACCATCAA CGGCCCGTGG GCATGGTCCA ACATCGACAC
    2301  CAGCAAAGTG AATTATGGTG TAACGGTACT GCCGACCTTC AAGGGTCAAC
    2351  CATCCAAACC GTTCGTTGGC GTGCTGAGCG CAGGTATTAA CGCCGCCAGT
    2401  CCGAACAAAG AGCTGGCAAA AGAGTTCCTC GAAAACTATC TGCTGACTGA
    2451  TGAAGGTCTG GAAGCGGTTA ATAAAGACAA ACCGCTGGGT GCCGTAGCGC
    2501  TGAAGTCTTA CGAGGAAGAG TTGGCGAAAG ATCCACGTAT TGCCGCCACT
    2551  ATGGAAAACG CCCAGAAAGG TGAAATCATG CCGAACATCC CGCAGATGTC
    2601  CGCTTTCTGG TATGCCGTGC GTACTGCGGT GATCAACGCC GCCAGCGGTC
    2651  GTCAGACTGT CGATGAAGCC CTGAAAGACG CGCAGACTAA TTCGAGCTCG
    2701  AACAACAACA ACAATAACAA TAACAACAAC CTCGGGATCG AGGGAAGGAT
    2751  TTCAGAATTC GGATCCTCTA GAGTCGACCT GCAGGCAAGC TTGGCACTGG
    2801  CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT
    2851  AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA
    2901  GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT
    2951  GGCAGCTTGG CTGTTTTGGC GGATGAGATA AGATTTTCAG CCTGATACAG
    3001  ATTAAATCAG AACGCAGAAG CGGTCTGATA AAACAGAATT TGCCTGGCGG
    3051  CAGTAGCGCG GTGGTCCCAC CTGACCCCAT GCCGAACTCA GAAGTGAAAC
    3101  GCCGTAGCGC CGATGGTAGT GTGGGGTCTC CCCATGCGAG AGTAGGGAAC
    3151  TGCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC
    3201  GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG
    3251  CCGGGAGCGG ATTTGAACGT TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC
    3301  AGGACGCCCG CCATAAACTG CCAGGCATCA AATTAAGCAG AAGGCCATCC
    3351  TGACGGATGG CCTTTTTGCG TTTCTACAAA CTCTTTTTGT TTATTTTTCT
    3401  AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG
    3451  CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT
    3501  CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC
    3551  CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA
    3601  GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT
    3651  TCGCCCCGAA GAACGTTCTC CAATGATGAG CACTTTTAAA GTTCTGCTAT
    3701  GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC
    3751  CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA
    3801  AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA
    3851  TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA
    3901  GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC
    3951  TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG
    4001  AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA
    4051  TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG
    4101  GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG
    4151  CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC
    4201  GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT
    4251  TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA
    4301  TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA
    4351  GTTTACTCAT ATATACTTTA GATTGATTTA CCCCGGTTGA TAATCAGAAA
    4401  AGCCCCAAAA ACAGGAAGAT TGTATAAGCA AATATTTAAA TTGTAAACGT
    4451  TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT
    4501  TTAACCAATA GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG
    4551  ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT
    4601  AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG
    4651  ATGGCCCACT ACGTGAACCA TCACCCAAAT CAAGTTTTTT GGGGTCGAGG
    4701  TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
    4751  TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA
    4801  AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
    4851  ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTAAAAGGA
    4901  TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT
    4951  GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
    5001  TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
    5051  AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT
    5101  CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
    5151  CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
    5201  CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT
    5251  GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
    5301  TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
    5351  TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA
    5401  GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
    5451  CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
    5501  CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT
    5551  CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
    5601  CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
    5651  TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC
    5701  TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
    5751  GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA
    5801  CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA
    5851  TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC
    5901  GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG
    5951  CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC
    6001  CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC
    6051  GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG CAGCGATTCA
    6101  CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG
    6151  CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT
    6201  CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG
    6251  GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA
    6301  TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG
    6351  TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC
    6401  TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC
    6451  GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA
    6501  GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC
    6551  GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA
    6601  TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA
    6651  ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC
    6701  GAAATT








[[Category:Plasmids]]
[[Category:Plasmids]][[Category:NEB Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:lacIq Promoter]][[Category:Ptac Promoter]][[Category:MCS]]
__NOTOC__
__NOTOC__

Latest revision as of 19:39, 17 June 2010

At a Glance

pMAL-pIII Image provided by NEB
Type

Cloning

Origin of Replication

M13, pMB1

Copy #
Markers

Ampicillin

Link to Sequence

NEB

Features

lacIq Promoter
Ptac Promoter
MCS

Accession Number


edit table



Notes

Isolated from E. coli strain ER2272 by standard DNA purification procedure. Rapid characterization of selected sequences by ELISA Alternative to chemical peptide synthesis Monovalency of MBP fusion allows accurate KD determination.

History

pMal-pIII is a derivative of pMal-p2. Kpn1/Acc651 and EagI sites have been introduced within the pIII leader to ease direct transfer of sequences selected from any of the PhD phage display peptide libraries into an expression vector.

Sources

New England Biolabs

References

[back to top]


See Help:References for how to manage references in EcoliWiki.


Sequence

      1  CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC 
     51  CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA 
    101  CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG 
    151  TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG 
    201  GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC 
    251  GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC 
    301  ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG 
    351  GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG 
    401  TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA 
    451  TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC 
    501  ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA 
    551  CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC 
    601  TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT 
    651  TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG 
    701  CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT 
    751  CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT 
    801  GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT 
    851  TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG 
    901  ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA 
    951  CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT 
   1001  CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG 
   1051  TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC 
   1101  GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 
   1151  AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG 
   1201  GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT 
   1251  GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC 
   1301  GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT 
   1351  AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG 
   1401  AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG 
   1451  GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA 
   1501  GCACTTCACC AACAAGGACC ATAGCATATG AAAAAATTAT TATTCGCAAT 
   1551  TCCTTTAGTG GTACCTTTCT ATTCTCACTC GGCCGATATG AAAATCGAAG 
   1601  AAGGTAAACT GGTAATCTGG ATTAACGGCG ATAAAGGCTA TAACGGTCTC 
   1651  GCTGAAGTCG GTAAGAAATT CGAGAAAGAT ACCGGAATTA AAGTCACCGT 
   1701  TGAGCATCCG GATAAACTGG AAGAGAAATT CCCACAGGTT GCGGCAACTG 
   1751  GCGATGGCCC TGACATTATC TTCTGGGCAC ACGACCGCTT TGGTGGCTAC 
   1801  GCTCAATCTG GCCTGTTGGC TGAAATCACC CCGGACAAAG CGTTCCAGGA 
   1851  CAAGCTGTAT CCGTTTACCT GGGATGCCGT ACGTTACAAC GGCAAGCTGA 
   1901  TTGCTTACCC GATCGCTGTT GAAGCGTTAT CGCTGATTTA TAACAAAGAT 
   1951  CTGCTGCCGA ACCCGCCAAA AACCTGGGAA GAGATCCCGG CGCTGGATAA 
   2001  AGAACTGAAA GCGAAAGGTA AGAGCGCGCT GATGTTCAAC CTGCAAGAAC 
   2051  CGTACTTCAC CTGGCCGCTG ATTGCTGCTG ACGGGGGTTA TGCGTTCAAG 
   2101  TATGAAAACG GCAAGTACGA CATTAAAGAC GTGGGCGTGG ATAACGCTGG 
   2151  CGCGAAAGCG GGTCTGACCT TCCTGGTTGA CCTGATTAAA AACAAACACA 
   2201  TGAATGCAGA CACCGATTAC TCCATCGCAG AAGCTGCCTT TAATAAAGGC 
   2251  GAAACAGCGA TGACCATCAA CGGCCCGTGG GCATGGTCCA ACATCGACAC 
   2301  CAGCAAAGTG AATTATGGTG TAACGGTACT GCCGACCTTC AAGGGTCAAC 
   2351  CATCCAAACC GTTCGTTGGC GTGCTGAGCG CAGGTATTAA CGCCGCCAGT 
   2401  CCGAACAAAG AGCTGGCAAA AGAGTTCCTC GAAAACTATC TGCTGACTGA 
   2451  TGAAGGTCTG GAAGCGGTTA ATAAAGACAA ACCGCTGGGT GCCGTAGCGC 
   2501  TGAAGTCTTA CGAGGAAGAG TTGGCGAAAG ATCCACGTAT TGCCGCCACT 
   2551  ATGGAAAACG CCCAGAAAGG TGAAATCATG CCGAACATCC CGCAGATGTC 
   2601  CGCTTTCTGG TATGCCGTGC GTACTGCGGT GATCAACGCC GCCAGCGGTC 
   2651  GTCAGACTGT CGATGAAGCC CTGAAAGACG CGCAGACTAA TTCGAGCTCG 
   2701  AACAACAACA ACAATAACAA TAACAACAAC CTCGGGATCG AGGGAAGGAT 
   2751  TTCAGAATTC GGATCCTCTA GAGTCGACCT GCAGGCAAGC TTGGCACTGG 
   2801  CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT 
   2851  AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA 
   2901  GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT 
   2951  GGCAGCTTGG CTGTTTTGGC GGATGAGATA AGATTTTCAG CCTGATACAG 
   3001  ATTAAATCAG AACGCAGAAG CGGTCTGATA AAACAGAATT TGCCTGGCGG 
   3051  CAGTAGCGCG GTGGTCCCAC CTGACCCCAT GCCGAACTCA GAAGTGAAAC 
   3101  GCCGTAGCGC CGATGGTAGT GTGGGGTCTC CCCATGCGAG AGTAGGGAAC 
   3151  TGCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC 
   3201  GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG 
   3251  CCGGGAGCGG ATTTGAACGT TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC 
   3301  AGGACGCCCG CCATAAACTG CCAGGCATCA AATTAAGCAG AAGGCCATCC 
   3351  TGACGGATGG CCTTTTTGCG TTTCTACAAA CTCTTTTTGT TTATTTTTCT 
   3401  AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 
   3451  CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 
   3501  CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC 
   3551  CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA 
   3601  GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT 
   3651  TCGCCCCGAA GAACGTTCTC CAATGATGAG CACTTTTAAA GTTCTGCTAT 
   3701  GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC 
   3751  CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 
   3801  AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA 
   3851  TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA 
   3901  GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC 
   3951  TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG 
   4001  AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 
   4051  TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 
   4101  GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG 
   4151  CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC 
   4201  GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT 
   4251  TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA 
   4301  TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 
   4351  GTTTACTCAT ATATACTTTA GATTGATTTA CCCCGGTTGA TAATCAGAAA 
   4401  AGCCCCAAAA ACAGGAAGAT TGTATAAGCA AATATTTAAA TTGTAAACGT 
   4451  TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT 
   4501  TTAACCAATA GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG 
   4551  ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT 
   4601  AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG 
   4651  ATGGCCCACT ACGTGAACCA TCACCCAAAT CAAGTTTTTT GGGGTCGAGG 
   4701  TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC 
   4751  TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA 
   4801  AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA 
   4851  ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTAAAAGGA 
   4901  TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT 
   4951  GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 
   5001  TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA 
   5051  AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 
   5101  CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 
   5151  CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC 
   5201  CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT 
   5251  GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 
   5301  TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT 
   5351  TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA 
   5401  GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 
   5451  CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG 
   5501  CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT 
   5551  CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 
   5601  CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA 
   5651  TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 
   5701  TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA 
   5751  GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA 
   5801  CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA 
   5851  TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC 
   5901  GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG 
   5951  CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC 
   6001  CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC 
   6051  GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG CAGCGATTCA 
   6101  CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG 
   6151  CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT 
   6201  CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG 
   6251  GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA 
   6301  TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG 
   6351  TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC 
   6401  TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC 
   6451  GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA 
   6501  GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC 
   6551  GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA 
   6601  TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA 
   6651  ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC 
   6701  GAAATT
Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=pMAL-pIII&oldid=1116704"